Results 51 to 60 of about 232,028 (265)

Comparison of CO2 gasification of coal in isothermal and non-isothermal conditions [PDF]

E3S Web of Conferences, 2019
The thermogravimetric method allows to carry out measurements both in isothermal conditions for a given temperature and in non-isothermal conditions at a set heating rate. The aim of the work was to compare the process of gasification of the same coal in
Czerski Grzegorz, Grzywacz Przemysław, Śpiewak Katarzyna   +2 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Kinetic screening of nucleic acid ligand libraries for hit identification, binding mode characterization, and sequence optimization

Sensing and Bio-Sensing Research
Binding and unbinding rates are important characteristics of molecular interactions as they dictate binding affinities and complex lifetimes. Particularly, the engineering and maturation of nucleic acid-based ligands for therapeutic or diagnostic ...
Mareike C. De Pascali, Nico Dreymann, Marcus M. Menger, Ulrich Rant, Wouter Engelen   +4 more
doaj   +1 more source

Kinetic Profile of the Antioxidant Activity of Propolis Extract: 2,2-Diphenyl-1-Picrylhydrazyl Radical Bleaching Assay

Journal of Apicultural Science, 2018
The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH•) bleaching assay kinetic profile.
Mirković Snježana, Rajković Katarina, Jeremić Sanja, Gavrilović Marijana, Tomić Ljiljana, Arsenijević Valentina Arsić, Krstić Boro   +6 more
doaj   +1 more source

Retrievals of chlorine chemistry kinetic parameters from Antarctic ClO microwave radiometer measurements [PDF]

Atmospheric Chemistry and Physics, 2011
Key kinetic parameters governing the partitioning of chlorine species in the Antarctic polar stratosphere were retrieved from 28 days of chlorine monoxide (ClO) microwave radiometer measurements made during the late winter/early spring of 2005 at Scott ...
S. Kremser, R. Schofield, G. E. Bodeker, B. J. Connor, M. Rex, J. Barret, T. Mooney, R. J. Salawitch, T. Canty, K. Frieler, M. P. Chipperfield, U. Langematz, W. Feng   +12 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Kinetic Measurement of the Erythrocyte Sedimentation Rate

Upsala Journal of Medical Sciences, 1994
A method for estimation of the erythrocyte sedimentation from repeated measurements during the initial part of the sedimentation phase is described. Based on the measurements between 18 and 24 minutes after the beginning of the reaction, the sedimentation at 60 minutes is estimated.
A, Kallner, P, Engervall, M, Björkholm
openaire   +2 more sources

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source