Results 21 to 30 of about 344,176 (312)

Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells

bioRxiv, 2022
Background Multiple genetic modifications may be required to develop potent off-the-shelf chimeric antigen receptor (CAR) T cell therapies. Conventional CRISPR-Cas nucleases install sequence-specific DNA double-strand breaks (DSBs), enabling gene knock ...
Viktor Glaser, Christian L. Flugel, Jonas Kath, Weijie Du, Vanessa Drosdek, Clemens Franke, M. Stein, A. Pruß, M. Schmueck-Henneresse, H. Volk, P. Reinke, D. Wagner   +11 more
semanticscholar   +1 more source

Nrf2 activation induces mitophagy and reverses Parkin/Pink1 knock down-mediated neuronal and muscle degeneration phenotypes

Cell Death and Disease, 2021
The balanced functionality of cellular proteostatic modules is central to both proteome stability and mitochondrial physiology; thus, the age-related decline of proteostasis also triggers mitochondrial dysfunction, which marks multiple degenerative ...
S. Gumeni, Eleni-Dimitra Papanagnou, M. Manola, I. Trougakos   +3 more
semanticscholar   +1 more source

Low-dose AAV-CRISPR-mediated liver-specific knock-in restored hemostasis in neonatal hemophilia B mice with subtle antibody response

Nature Communications, 2022
AAV-delivered CRISPR/Cas9 (AAV-CRISPR) has shown promising potentials in preclinical models to efficiently insert therapeutic gene sequences in somatic tissues.
Xiangjun He, Zhenjie Zhang, Jun Xue, Yaofeng Wang, Siqi Zhang, Junkang Wei, Chenzi Zhang, Jue Wang, Brian Anugerah Urip, Chun Christopher Ngan, Junjiang Sun, Yuefeng Li, Zhiqian Lu, Hui Zhao, D. Pei, Chi-kong Li, Bo Feng   +16 more
semanticscholar   +1 more source

Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing

Nature Cell Biology, 2020
CRISPR–Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches.
B. Artegiani, D. Hendriks, J. Beumer, R. Kok, Xuan Zheng, I. Joore, S. M. Chuva de Sousa Lopes, Jeroen van Zon, S. Tans, H. Clevers   +9 more
semanticscholar   +1 more source

Pooled Knock-In Targeting for Genome Engineering of Cellular Immunotherapies

Cell, 2020
SUMMARY Adoptive transfer of genetically-modified immune cells holds great promise for cancer immunotherapy. CRISPR knock-in targeting can improve cell therapies, but more high-throughput methods are needed to test which knock-in gene constructs most ...
Theodore L. Roth, P. J. Li, Franziska Blaeschke, Jasper F. Nies, Chun Jimmie Ye, A. Marson   +5 more
semanticscholar   +1 more source

Knock combustion characteristics of an opposed‐piston two‐stroke gasoline engine

Energy Science & Engineering, 2022
The spark plug of an opposed‐piston two‐stroke (OP2S) gasoline engine is arranged on the side wall of the cylinder liner, far from the center of the combustion chamber; the ignition core of the mixture is offset, the flame propagation distance is ...
Fukang Ma, Jianwei Zhang, Fang Wang
doaj   +1 more source

Knock-in models related to Alzheimer’s disease: synaptic transmission, plaques and the role of microglia

Molecular Neurodegeneration, 2021
Background Microglia are active modulators of Alzheimer’s disease but their role in relation to amyloid plaques and synaptic changes due to rising amyloid beta is unclear.
Diana P Benitez, Shenyi Jiang, Jack I. Wood, Rui Wang, Chloe M. Hall, Carlijn Peerboom, N. Wong, Katie M Stringer, K. Vitanova, Victoria C. Smith, Dhaval D. Joshi, T. Saito, T. Saido, J. Hardy, Jörg Hanrieder, B. de Strooper, Dervis A. Salih, T. Tripathi, F. Edwards, D. Cummings   +19 more
semanticscholar   +1 more source

Deep Learning for Knock Occurrence Prediction in SI Engines

Energies, 2022
This research aims to predict knock occurrences by deep learning using in-cylinder pressure history from experiments and to elucidate the period in pressure history that is most important for knock prediction. Supervised deep learning was conducted using
Haruki Tajima, Takuya Tomidokoro, Takeshi Yokomori   +2 more
doaj   +1 more source

Effect of Spark Ignition Timing and Water Injection Temperature on the Knock Combustion of a GDI Engine

Energies, 2020
A turbocharged downsizing spark ignition (SI) engine cooperating with an in-cylinder direct injection technology is one of the most effective ways to improve the power and economy of gasoline engines. However, engine knock has limited the application and
Aqian Li, Zhaolei Zheng
doaj   +1 more source

KNOCK, KNOCK, KNOCKING

Entrelinhas, 2021
poema para a 'Seção Livre'
openaire   +2 more sources