A Career's Work, the l-Arabinose Operon: How It Functions and How We Learned It. [PDF]
Very few labs have had the good fortune to have been able to focus for more than 50 years on a relatively narrow research topic and to be in a field in which both basic knowledge and the research technology and methods have progressed as rapidly as they have in molecular biology. My research group, first at Brandeis University and then at Johns Hopkins
Schleif R.
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Coordination of Enzyme Synthesis in the l-Arabinose Operon in Escherichia coli [PDF]
Abstract Addition of manganous ion to growing cultures of Escherichia coli B/r results in at least a 2-fold increase in the specific activity of l-arabinose isomerase. The effect of Mn2+ is to cause the synthesis of an enzyme with a greater intrinsic activity and heat stability, but not to change the quantity of l-arabinose isomerase produced.
James W. Patrick+3 more
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Regulation of the l-Arabinose Operon BAD in Vitro
Abstract A DNA-directed cell-free system to study the l-arabinose BAD operon has been further developed so that the rate of synthesis of enzymes coded for by the structural genes in the operon in vitro is about 5% the in vivo rate. l-Arabinose isomerase and l-ribulokinase, the products of the araA and araB genes, are synthesized at the same rate ...
Gary Wilcox+3 more
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Regulation of the l-arabinose operon of Escherichia coli [PDF]
Over forty years of research on the L-arabinose operon of Escherichia coli have provided insights into the mechanism of positive regulation of gene activity. This research also discovered DNA looping and the mechanism by which the regulatory protein changes its DNA-binding properties in response to the presence of arabinose.
Robert Schleif
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Mutations in the L-arabinose operon of Escherichia coli B/r with reduced initiator function [PDF]
Partial reversion mutants derived from a strain containing a strongly polar initiator-defective mutation (araI1036) in the L-arabinose operon were found to have several characteristics expected of mutants with reduced initiator function. These reversion mutations are cotransduced with the ara region and are probably within the araI region. Furthermore,
Iris L. Gonzalez, David E. Sheppard
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Regulatory properties of araC(c) mutants in the L-arabinose operon of escherichia coliB/r [PDF]
Merodiploids containing a high-constitutive and a low-constitutive araC(c) allele were assayed for constitutive expression of the ara operon. Low-constitutive araC(c) alleles either were unable to repress the constitutive rate of ara operon expression exhibited by by high-constitutive araC(c) alleles or achieved a partial repression of the high ...
K. MacInnes+2 more
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Fine-Structure Deletion Map of the Escherichia coli L-Arabinose Operon [PDF]
A fine-structure deletion map of the L-arabinose operon of E. coli was constructed by mapping deletion endpoints against point mutations. Of 350 independent deletions with average endpoint separation of ten nucleotides, 51 ended in the control region between the C and B
Robert Schleif
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Electron microscopy of gene regulation: The
Using high magnification electron microscopy, we have observed protein complexes specifically bound to homogeneous DNA fragments containing the regulatory region of the Escherichia coli L -arabinose operon.
Jay Hirsh, Robert Schleif
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Polarity in Gene araB of the
A series of mutations are described which map in the araB gene of the l -arabinose operon and exert a polar effect on gene araA , the structural gene for the l -arabinose isomerase.
David E. Sheppard, Derinda A. Walker
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THE L-ARABINOSE OPERON IN Escherichia coli B/r: A GENETIC DEMONSTRATION OF TWO FUNCTIONAL STATES OF THE PRODUCT OF A REGULATOR GENE [PDF]
The product of the regulator gene araC in the L-arabinose gene complex exists in two functional states: P1, the repressor, and P2, the activator, presumably in equilibrium with each other, and with P1 and P2 attached to their respective controlling sites, araO , the operator, and ...
Ellis Englesberg+2 more
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