Results 111 to 120 of about 8,929 (131)
High-affinity l-arabinose transport operon
Journal of Molecular Biology, 1987 Various portions of the "high-affinity" L-arabinose transport operon were cloned into the plasmid expression vector pKK223-3 and the operon-encoded protein products were identified. The results indicate that three proteins are encoded by this operon.
J. Benjamin Scripture +7 more
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Proflavin-induced mutations in the l-arabinose operon of Escherichia coli
Journal of Molecular Biology, 1969 Abstract The enzymic activities of mutant gene products, as well as the enzymic activities of products from adjacent genes, were assayed in strains harboring proflavin-induced frame-shift mutations in the L-arabinose (ara) operon. Such mutations in araB were found to depress strongly the expression of the adjacent gene, araA. Frame-shift mutations in
Suzanne Sesnowitz-Horn +1 more
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The regulatory region of the l-arabinose operon: A physical, genetic and physiological study
Journal of Molecular Biology, 1975 Abstract A fine-structure physical and genetic map was constructed of a 1000 base-pair region of the l -arabinose operon of Escherichia coli. This region consists of the ara regulatory sequences contained between the araC and araB genes and portions of these flanking genes.
Robert Schleif, John T. Lis
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l-arabinose operon messenger of Escherichia coli
Journal of Molecular Biology, 1971 Abstract Synthesis of messenger RNA from the positively controlled l -arabinose operon is shown to be induced by the presence of arabinose in the medium. Messengers from the arabinose and lactose operons are translated with comparable efficiencies.
Robert Schleif
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Journal of Molecular Biology, 1974 Abstract The DNA binding properties of the araC protein in the absence of l -arabinose have been studied in Escherichia coli using the nitrocellulose membrane filter technique. Equilibrium competition experiments demonstrate that the araC protein binds specifically to the ara operator. The apparent Km of the interaction is 1 × 10−12 m at 20 °C. The
Gary Wilcox +3 more
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Bistability and Asynchrony in a Boolean Model of the l-arabinose Operon in Escherichia coli
Bulletin of Mathematical Biology, 2017 The lactose operon in Escherichia coli was the first known gene regulatory network, and it is frequently used as a prototype for new modeling paradigms. Historically, many of these modeling frameworks use differential equations. More recently, Stigler and Veliz-Cuba proposed a Boolean model that captures the bistability of the system and all of the ...
Andy Jenkins, Matthew Macauley
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Journal of Molecular Biology, 1984 DNA binding properties of the proteins required for induction of the Escherichia coli L-arabinose operon were measured using a polyacrylamide gel electrophoresis assay. The mechanisms of induction and repression were studied by observing the multiple interactions of RNA polymerase, cyclic AMP receptor protein and araC protein with short DNA fragments ...
William Hendrickson, Robert Schleif
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Enzyme and Microbial Technology, 2001 The Escherichia coli araBAD operon consists of three genes encoding three enzymes that convert L-arabinose to D-xylulose-5 phosphate. In this paper we report that the genes of the E. coli araBAD operon have been expressed in Saccharomyces cerevisiae using strong promoters from genes encoding S.
Miroslav Sedlák, Nancy W. Y. Ho
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The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r
Archives of Microbiology, 1976 A series of deletions beginning in the leu operon and continuing into the araC gene and also into the ara controlling site region were analyzed in reciprocal merodiploids, e.g., F' A2Cc67/B24delta719, F' B24delta719/A2Cc67, for their effects on catabolite deactivation (CD).
Richard Bass +3 more
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