Results 121 to 130 of about 8,929 (131)
Abstract A large number of high-level and low-level constitutive mutations in the araC gene of Escherichia coli were shown by deletion mapping to lie almost exclusively in two regions of the araC gene. Recombination data show that the high-level constitutive mutations are located within two very small regions, each probably less than ten base-pairs ...
Neil M. Nathanson, Robert Schleif
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Hybrid plasmids were constructed from fragments of F'ara episomes formed by the restriction endonuclease EcoRI and a linear form of the plasmid ColE1 created by cleavage with EcoRI. Hybrid plasmids were constructed containing the entire ara region or the ara region with various parts deleted. E.
Linda J. Wallace, Gary Wilcox
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Regulatory Mutants Of The L-arabinose Operon Of Escherichia Coli B/r.
PhD ; Zoology ; University of Michigan, Horace H. Rackham School of Graduate Studies ; http://deepblue.lib.umich.edu/bitstream/2027.42/190699/2/7425189 ...
M. Ellin Doyle
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Abstract A DNA fragment containing the l -arabinose operon regulatory region of Escherichia coli was purified from DNA heteroduplexes formed between opposite strands of two non-defective ara transducing phage. The phage and arabinose gene orientation is such that the heteroduplex contains two single-stranded “bubbles”.
John T. Lis, Robert Schleif
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Induction of the L-arabinose operon
Robert Schleif
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Regulation of the l-arabinose transport operons in Escherichia coli
Journal of Molecular Biology, 1981l-Arabinose is transported into Escherichia coli via two independent transport systems, a system possessing relatively low affinity for arabinose, the araE system, and a system of higher affinity for arabinose, the araFG system. In the work reported here we demonstrate that insertion of the Mu-lac bacteriophage isolated by Casadaban & Cohen (1979 ...
David J Kolodrubetz, Robert Schleif
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Upstream repression and CRP stimulation of the Escherichia colil-arabinose operon
Journal of Molecular Biology, 1984Repression of the Escherichia coli araBAD promoter, PBAD, was studied using a mutant PBAD promoter (cip-5) that is expressed in the absence of the two proteins required for PBAD induction, AraC protein and the cyclic AMP receptor protein (CRP-cAMP). Like the wild type promoter, cip-5 was repressed by AraC protein, and this repression required a site ...
Steven Hahn+2 more
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The araBAD operon of Salmonella typhimurium LT2
Gene, 1985Hybrid plasmids containing the araBAD operon of Salmonella typhimurium LT2 were characterized by Southern blot and genetic analyses. The nucleotide sequence of araB was determined. The araB gene product, ribulokinase (EC 2.7.1.16), was purified and the results of amino acid composition analysis and partial amino acid sequence are in agreement with ...
Hun-Chi Lin+2 more
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24 Regulation of the L-Arabinose Catabolic Operon araBAD
Robert Schleif
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Synchrony in a Boolean Network of the L-arabinose Operon in Escherichia coli
Andy Jenkins, Matthew Macauley
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