The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression [PDF]
The Bacillus subtilis L-arabinose metabolic genes araA, araB and araD, encoding L-arabinose isomerase, L-ribulokinase and L-ribulose-5-phosphate 4-epimerase, respectively, have been cloned previously and the products of araB and araD were shown to be functionally homologous to their Escherichia coli counterparts by complementation experiments.
Isabel Sá‐Nogueira+3 more
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Regulatory Gene Control of Transcription of the l-Arabinose Operon in Escherichia coli
Abstract The l-arabinose operon is under both positive and negative control by the product of its regulatory gene, araC. We have measured transcription of the arabinose genes by hybridization of 3H-labeled messenger RNA to single-stranded DNA from an arabinose transducing phage in order to study the effect of the regulatory gene on transcription.
Joseph Power, Joseph Irr
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Mutations in the
Two independent mutants resistant to l -arabinose inhibition only in the presence of d -glucose were isolated from an l -arabinose-sensitive strain containing the araD139 mutation.
Robert P. Gendron, David E. Sheppard
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The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation. [PDF]
The locations of DNA binding by the proteins involved with positive and negative regulation of transcription initiation of the L-arabinose operon in Escherichia coli have been determined by the DNase I protection method. Two cyclic AMP receptor protein sites were found, at positions -78 to -107 and -121 to -146, an araC protein--arabinose binding site ...
S Ogden+4 more
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Metabolite gene regulation of the L-arabinose operon in Escherichia coli with indoleacetic acid and other indole derivatives. [PDF]
The ability of indole derivatives to facilitate RNA polymerase transcription of the L-arabinose operon in Escherichia coli was shown to require the catabolite activator protein (CAP) as well as the araC gene product. Adenosine 3',5'-monophosphate (cAMP) was not obligatory for araBAD transcription when the cells were grown in the presence of 1 mM indole-
Ellis L. Kline+4 more
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Transcriptional Control in the
The structural genes involved in l -arabinose metabolism are regulated by the protein product of the araC gene. This protein functions as both an activator and repressor of enzyme synthesis in this gene complex.
P. Patrick Cleary, Ellis Englesberg
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Synchrony in a Boolean network model of the L-arabinose operon in Escherichia coli
12 pages, 4 ...
Andy Jenkins, Matthew Macauley
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Control of Expression of the
Expression of the l -arabinose operon in Escherichia coli B/r is dependent on the temperature of growth of the araC mutants reported in this paper. Analysis of these temperature-sensitive regulatory mutants indicates that both repressor and activator activities are ...
Joseph Irr, Ellis Englesberg
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Induction Kinetics of the
After addition of l -arabinose to growing Escherichia coli , the l -ribulokinase (EC 2.7.1.16) and l -arabinose isomerase (EC 5.3.1.4) first appear at about 0.7 and 1.4 min, respectively.
Robert Schleif+3 more
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Interaction between mutant alleles of araC of the Escherichia coli B/r L-arabinose operon [PDF]
Strains were constructed that contain mutational alterations affecting two distinct functional domains within the araC gene protein. The araCi (catabolite repression insensitivity) and araCh (catabolite repression hypersensitivity) mutations were used to alter the catabolite repression sensitivity domain, and mutation to D-fucose resistance was used to
David E. Sheppard+2 more
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