Results 51 to 60 of about 8,929 (131)
Two Key Ferredoxins for Nitrogen Fixation Have Different Specificities and Biophysical Properties
Chemistry – A European Journal, Volume 31, Issue 37, July 2, 2025.Two ferredoxins, FdC and FdN, are collectively essential for the process of nitrogen fixation by the iron nitrogenase in R. capsulatus. We explore the biophysical factors, through spectroscopic and structural characterizations, which determine ferredoxin functionality and pathway specificity. The structure of FdC (PDB: 9I2A) and model of FdN (AF‐D5ARY6)
Holly Addison +5 more
wiley +1 more source
Influence of Different Transposon Families on Genomic Stability of Shewanella oneidensis MR1
Microbial Biotechnology, Volume 18, Issue 7, July 2025.This study aimed for targeted genome stabilisation in Shewanella oneidensis MR1 through two approaches: complete ISSOD2 inactivation via CRISPR‐deaminase‐mediated stop codon integration, and megaplasmid size reduction (35% deletion). Analysis revealed ISSOD2 as the most active transposon family (84% activity) and identified transposition hotspots for ...
Benjamin Fritz +2 more
wiley +1 more source
B Cells as a Host of Persistent Salmonella Typhimurium
Immunology, Volume 175, Issue 3, Page 292-299, July 2025.B cells: a niche for persistent Salmonella enterica serovar Typhimurium (S. Tm). In vitro, within B cells and in the absence of antibiotics, a persistent population of S. Tm emerges, where the SehA/B gene is implicated. Additionally, a replicative population of S. Tm is also present. Upon antibiotic administration, approximately 90% of the S.
Alonso D. Cruz‐Cruz +4 more
wiley +1 more source
Molecular Microbiology, Volume 124, Issue 1, Page 20-39, July 2025.A novel TetR‐like regulator (EppR) has been identified to repress genes encoding DNA polymerase V in Acinetobacter baumannii through the direct binding of a conserved EppR motif in their promoters. EppR works with previously identified regulator UmuDAb to serve as co‐regulators of these genes. In response to DNA damage and/or environmental stress, both
Brian Nguyen +6 more
wiley +1 more source
Assessment and Mitigation of CRISPR‐Cas9‐Induced Nontargeted Translocations
Advanced Science, Volume 12, Issue 21, June 5, 2025.Various inverted repeat elements are dispersed throughout genomes. This study reveals that these elements can cause significant chromosomal rearrangements when CRISPR editing occurs in their proximity. The risk can be mitigated by incorporating inverted repeat‐homologous segments into the CRISPR system, which represents a promising strategy for ...
Zhiyang Hou +6 more
wiley +1 more source
Structural and Biochemical Characterization of a Widespread Enterobacterial Peroxidase Encapsulin
Advanced Science, Volume 12, Issue 21, June 5, 2025.Encapsulins are self‐assembling bacterial protein compartments loaded with cargo enzymes. The most abundant encapsulin cargo class are Dye‐decolorizing Peroxidases (DyPs). In this study, we structurally and biochemically characterize a DyP encapsulin found in many enterobacteria.
Natalia C. Ubilla‐Rodriguez +2 more
wiley +1 more source
Microbial Biotechnology, Volume 18, Issue 6, June 2025.Molecular and functional experiments highlight the relevance of species‐specific polymorphisms in a B12 riboswitch of the Mycobacterium genus. This information provided proof‐of‐concept to engineer a whole‐cell biosensor capable of detecting vitamin B12. ABSTRACT Vitamin B12 metabolism differs among members of the Mycobacterium genus.
Elena Campos‐Pardos +6 more
wiley +1 more source
Environmental Microbiology, Volume 27, Issue 5, May 2025.Exposure of Pseudomonas putida KT2440 to toxic metals activates genes PP_1663, PP_5002, PP_5337, and the two‐component system RoxSR, possibly linked to membrane integrity, FeS cluster maintenance, and DNA transcription, offering new insights into bacterial adaptation to metal tolerance.
Kevin Royet +8 more
wiley +1 more source
Microbial Biotechnology, Volume 18, Issue 5, May 2025.A new route for PET upcycling to 2,5‐furandicarboxylic acid (FDCA) was developed using an engineered Pseudomonas umsongensis GO16. This work will expand the scope of biotechnological upcycling of waste residues, contributing to the sustainable vision of the circular bioeconomy.
Rhys Orimaco +7 more
wiley +1 more source
Molecular Microbiology, Volume 123, Issue 5, Page 439-453, May 2025.The Roc system represents a highly interconnected but incomplete network of two‐component regulatory systems involved in the virulence of Pseudomonas aeruginosa. Our work has enabled us to identify the missing RocA3 regulator, to propose new players in the system and to delineate their conservation between clades of the species.
Victor Simon +3 more
wiley +1 more source