Results 111 to 120 of about 4,321,036 (262)
, 1951 Memorandum listing Laboratory Personnel of the MIT Digital Computer ...
Lincoln Laboratory Administrative Staff +1 more
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FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Misidentification and other preanalytical errors [PDF]
Journal of Medical Biochemistry, 2008 The largest number of laboratory errors occur in the preanalytical phase and are mainly due to educational and organizational reasons. The experience of our institution, as well as the results of an Italian interlaboratory effort to detect and reduce ...
Bonini Pierangelo +3 more
doaj
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
University of California Radiation Laboratory Reports UCRL-1854
, 1952 Quarterly summary report of studies conducted through the University of California Radiation Laboratory.
University of California. Radiation Laboratory.
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FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Clinical Infection in PracticeIn 2023, the World Health Organization estimates that 1 in every 10 patients experiences harm from unsafe care, with 3 million deaths occurring yearly from the same. Over half the cases of patient harm are preventable and resultant from errors. As 70% of
John C. Lam, Deirdre L. Church
doaj +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
University of California Radiation Laboratory Reports UCRL-1632
, 1952 Quarterly summary report of studies conducted through the Radiation Laboratory, University of California.
University of California. Radiation Laboratory.
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FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source