Lac repressor mediated DNA looping: Monte Carlo simulation of constrained DNA molecules complemented with current experimental results. [PDF]
PLoS ONE, 2014 Tethered particle motion (TPM) experiments can be used to detect time-resolved loop formation in a single DNA molecule by measuring changes in the length of a DNA tether.
Yoav Y Biton +3 more
doaj +6 more sources
A Lac Repressor-Inducible Baculovirus Expression Vector for Controlling Adeno-Associated Virus Capsid Ratios [PDF]
Viruses, 2023 The baculovirus expression vector (BEV) system is an efficient, cost-effective, and scalable method to produce recombinant adeno-associated virus (rAAV) gene therapy vectors.
Jeffrey Slack +2 more
doaj +2 more sources
Ligand-specific changes in conformational flexibility mediate long-range allostery in the lac repressor [PDF]
Nature Communications, 2023 Biological regulation ubiquitously depends on protein allostery, but the regulatory mechanisms are incompletely understood, especially in proteins that undergo ligand-induced allostery with few structural changes. Here we used hydrogen-deuterium exchange
Anum Glasgow +5 more
doaj +2 more sources
From adjacent activation in E. coli and DNA cyclization to eukaryotic enhancers : the elements of a puzzle. [PDF]
Frontiers in Genetics, 2014 DNA cyclization, E. coli lac repressor binding to two spaced lac operators and repression enhancement can be successfully used for a better understanding of the conditions required for interaction between eukaryotic enhancers and the machinery of ...
Michèle eAMOUYAL
doaj +2 more sources
Tethering sister centromeres to each other suggests the spindle checkpoint detects stretch within the kinetochore. [PDF]
PLoS Genetics, 2014 The spindle checkpoint ensures that newly born cells receive one copy of each chromosome by preventing chromosomes from segregating until they are all correctly attached to the spindle.
Natalie J Nannas, Andrew W Murray
doaj +6 more sources
Concentration and length dependence of DNA looping in transcriptional regulation. [PDF]
PLoS ONE, 2009 In many cases, transcriptional regulation involves the binding of transcription factors at sites on the DNA that are not immediately adjacent to the promoter of interest.
Lin Han +6 more
doaj +10 more sources
In vitro transcription accurately predicts lac repressor phenotype in vivo in Escherichia coli [PDF]
PeerJ, 2014 A multitude of studies have looked at the in vivo and in vitro behavior of the lac repressor binding to DNA and effector molecules in order to study transcriptional repression, however these studies are not always reconcilable.
Matthew Almond Sochor
doaj +3 more sources
Interplay of protein and DNA structure revealed in simulations of the lac operon. [PDF]
PLoS ONE, 2013 The E. coli Lac repressor is the classic textbook example of a protein that attaches to widely spaced sites along a genome and forces the intervening DNA into a loop.
Luke Czapla +3 more
doaj +7 more sources
Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI. [PDF]
PLoS ONE, 2018 The lactose operon repressor protein LacI has long served as a paradigm of the bacterial transcription factors. However, the mechanisms whereby LacI rapidly locates its cognate binding site on the bacterial chromosome are still elusive.
Kalle Kipper +7 more
doaj +2 more sources
The Hinge Region Strengthens the Nonspecific Interaction between Lac-Repressor and DNA: A Computer Simulation Study. [PDF]
PLoS ONE, 2016 LacI is commonly used as a model to study the protein-DNA interaction and gene regulation. The headpiece of the lac-repressor (LacI) protein is an ideal system for investigation of nonspecific binding of the whole LacI protein to DNA. The hinge region of
Lili Sun +8 more
doaj +2 more sources