Optimized expression and purification of biophysical quantities of Lac repressor and Lac repressor regulatory domain. [PDF]
Protein Expr Purif, 2016 The recombinant production of Lac repressor (LacI) in Escherichia coli is complicated by its ubiquitous use as a regulatory element in commercially-available expression vectors and host strains. While LacI-regulated expression systems are often used to produce recombinant LacI, the product can be heterogeneous and unsuitable for some studies ...
Stetz MA, Carter MV, Wand AJ.
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Interconvertible lac repressor-DNA loops revealed by single-molecule experiments. [PDF]
PLoS Biology, 2008 At many promoters, transcription is regulated by simultaneous binding of a protein to multiple sites on DNA, but the structures and dynamics of such transcription factor-mediated DNA loops are poorly understood.
Oi Kwan Wong +3 more
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Multilevel deconstruction of the In vivo behavior of looped DNA-protein complexes. [PDF]
PLoS ONE, 2007 Protein-DNA complexes with loops play a fundamental role in a wide variety of cellular processes, ranging from the regulation of DNA transcription to telomere maintenance.
Leonor Saiz, Jose M G Vilar
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Modeling the Lac repressor-operator assembly: the influence of DNA looping on Lac repressor conformation. [PDF]
Proc Natl Acad Sci U S A, 2006 Repression of transcription of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by the simultaneous binding of LacR to two operators and the formation of a DNA loop.
Swigon D, Coleman BD, Olson WK.
europepmc +4 more sources
lac repressor is an antivirulence factor of Salmonella enterica: its role in the evolution of virulence in Salmonella. [PDF]
PLoS ONE, 2009 The genus Salmonella includes many pathogens of great medical and veterinary importance. Bacteria belonging to this genus are very closely related to those belonging to the genus Escherichia.
Sandeepa M Eswarappa +4 more
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Insights on the effect of macromolecular crowding on transcription and its regulation [PDF]
QRB DiscoveryTranscription of DNA into RNA is a fundamental cellular process upon which life depends. It is tightly regulated in several different ways, and among the most important mechanisms are protein-induced topological changes in DNA such as looping.
Wenxuan Xu +4 more
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Introduction to a special issue of Magnetic Resonance in honour of Robert Kaptein at the occasion of his 80th birthday [PDF]
Magnetic Resonance, 2021 This publication, in honour of Robert Kaptein's 80th birthday, contains contributions from colleagues, many of whom have worked with him, and others who admire his work and have been stimulated by his research.
R. Boelens +3 more
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The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells
Frontiers in Genetics, 2021 BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki +8 more
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High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. [PDF]
PLoS ONE, 2013 Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.
Yao Nie +6 more
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PLoS ONE, 2021 The cAMP-CRP regulon coordinates transcription regulation of several energy-related genes, the lac operon among them. Lactose, or IPTG, induces the lac operon expression by binding to the LacI repressor, and releasing it from the promoter sequence.
Chunghwan Ro +2 more
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