Results 21 to 30 of about 31,663 (206)

Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Microbial Cell Factories, 2020
Background The genome-integrated T7 expression system offers significant advantages, in terms of productivity and product quality, even when expressing the gene of interest (GOI) from a single copy.
Artur Schuller, Monika Cserjan-Puschmann, Christopher Tauer, Johanna Jarmer, Martin Wagenknecht, Daniela Reinisch, Reingard Grabherr, Gerald Striedner   +7 more
doaj   +1 more source

Regulatory control and the costs and benefits of biochemical noise [PDF]

, 2008
Experiments in recent years have vividly demonstrated that gene expression can be highly stochastic. How protein concentration fluctuations affect the growth rate of a population of cells, is, however, a wide open question.
A Bar-Even, A Becskei, A Sigal, A Wagner, Aviv Regev, DM Wolf, DS Falconer, DT Gillespie, DW Austin, E Dekel, E Dekel, E Kussell, E Kussell, E Levine, E Ziv, EM Ozbudak, G Yagil, H Risken, J Elf, J Paulsson, J Seger, J Yu, JL Ingraham, JM Pedraza, JM Raser, JR Sadler, JRS Newman, L Cai, M Kaern, M Thattai, MA Savageau, MA Savageau, MB Elowitz, MB Elowitz, MW van der Woude, N Rosenfeld, NQ Balaban, P Kolkhof, PB Detwiler, Pieter Rein ten Wolde, S Gavrilets, S Tănase-Nicola, Sorin Tănase-Nicola, T Kalisky, T Lu, T Shibata, W Gilbert, X Zhang   +47 more
core   +9 more sources

What makes the lac-pathway switch: identifying the fluctuations that trigger phenotype switching in gene regulatory systems [PDF]

, 2014
Multistable gene regulatory systems sustain different levels of gene expression under identical external conditions. Such multistability is used to encode phenotypic states in processes including nutrient uptake and persistence in bacteria, fate ...
Berg, Johannes, Bhogale, Prasanna M., Sorg, Robin A., Veening, Jan-Willem   +3 more
core   +2 more sources

Development of a Positive-readout Mouse Model of siRNA Pharmacodynamics

Molecular Therapy: Nucleic Acids, 2013
Development of RNAi-based therapeutics has the potential to revolutionize treatment options for a range of human diseases. However, as with gene therapy, a major barrier to progress is the lack of methods to achieve and measure efficient delivery for ...
Mark Stevenson, Robert Carlisle, Ben Davies, Chris Preece, Michelle Hammett, Wei-li Liu, Kerry David Fisher, Amy Ryan, Heidi Scrable, Leonard William Seymour   +9 more
doaj   +1 more source

Sequence Dependence of Transcription Factor-Mediated DNA Looping [PDF]

, 2012
DNA is subject to large deformations in a wide range of biological processes. Two key examples illustrate how such deformations influence the readout of the genetic information: the sequestering of eukaryotic genes by nucleosomes, and DNA looping in ...
Barry, Bellomy, Bellomy, Brenowitz, Butler, Chen, Chen, Cloutier, Cloutier, Drew, Du, Echols, Edelman, Finzi, Frank, Garcia, Geggier, Han, Hirsh, Hogan, Hsieh, Jacobson, Krämer, Lewis, Lowary, Luger, Luijsterburg, Martin Lindén, Matthews, Mehta, Milstein, Morgan, Müller, Newman, Normanno, Oehler, Olson, Peters, Plischke, Ptashne, Rippe, Rob Phillips, Rutkauskas, Saiz, Schafer, Schleif, Segall, Shimada, Stephanie Johnson, Swigon, Tolhuis, Towles, Vanzi, Villa, Watson, Whitson, Whitson, Widom, Wong, Xu, Yin, Zhan, Zhang   +62 more
core   +4 more sources

Tuning up Transcription Factors for Therapy

Molecules, 2020
The recent developments in the delivery and design of transcription factors put their therapeutic applications within reach, exemplified by cell replacement, cancer differentiation and T-cell based cancer therapies.
Attila Becskei
doaj   +1 more source

Structure and function of negative feedback loops at the interface of genetic and metabolic networks [PDF]

, 2006
The molecular network in an organism consists of transcription/translation regulation, protein-protein interactions/modifications and a metabolic network, together forming a system that allows the cell to respond sensibly to the multiple signal molecules
Andersson, Anna M. C., Krishna, Sandeep, Semsey, Szabolcs, Sneppen, Kim   +3 more
core   +4 more sources

How lac repressor recognizes lac operator. [PDF]

Proceedings of the National Academy of Sciences, 1978
Nucleotide analogs were substituted for unmodified nucleotides at specific sites in the lac operator sequence by a combination of chemical and enzymatic procedures. The nitrocellulose filter assay was used to study the interactions of these modified operators with wild-type (SQ) and tight-binding (QX86) lac repressors.
D V, Goeddel, D G, Yansura, M H, Caruthers   +2 more
openaire   +2 more sources

Dynamic model of gene regulation for the lac operon [PDF]

, 2011
Gene regulatory network is a collection of DNA which interact with each other and with other matter in the cell. The lac operon is an example of a relatively simple genetic network and is one of the best-studied structures in the Escherichia coli ...
Asma Ben-Halim, Bower J, Kloeden P E, Maia Angelova, Murray J D, Ptashne M, Stark H, van Hoek M   +7 more
core   +1 more source

Lac repressor at last [PDF]

Structure, 1996
Crystal and cocrystal structures of the LacI and PurR repressors reveal a novel use of hinge alpha helices which bind in the minor groove of the operator and mediate transmission of the allosteric signals that modulate DNA-binding activity.
openaire   +2 more sources