Results 31 to 40 of about 31,663 (206)
Lac repressor—operator complex
Current Opinion in Structural Biology, 1997 For many years the lac operon of Escherichia coli has been the paradigm for gene regulation. Recently, the structures of the lac repressor core bound to isopropyl-beta-D-1-thiogalactoside (IPTG), the intact apo lac repressor, the intact lac repressor complexes with IPTG and a 21-base-pair symmetric operator, and the refined headpiece of the repressor ...
M A, Kercher, P, Lu, M, Lewis
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lac repressor-lac operator interaction: NMR observations. [PDF]
Proceedings of the National Academy of Sciences, 1982 We show here the changes in the NMR spectra of the Escherichia coli lac repressor when bound to isolated lac operator DNA. The observations focus on the aromatic residues--four tyrosines and a single histidine--in the amino-terminal DNA binding domain of the lac repressor.
H, Nick +7 more
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A Type System for a Stochastic CLS [PDF]
, 2009 The Stochastic Calculus of Looping Sequences is suitable to describe the evolution of microbiological systems, taking into account the speed of the described activities.
Angelo Troina +3 more
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Noise contributions in an inducible genetic switch: a whole-cell simulation study. [PDF]
PLoS Computational Biology, 2011 Stochastic expression of genes produces heterogeneity in clonal populations of bacteria under identical conditions. We analyze and compare the behavior of the inducible lac genetic switch using well-stirred and spatially resolved simulations for ...
Elijah Roberts +4 more
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Targets for the MalI repressor at the divergent Escherichia coliK-12malX-malI promoters [PDF]
, 2010 Random mutagenesis has been used to identify the target DNA sites for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters. The malX promoter is repressed by MalI binding to a DNA site located from position -24 to position -9 ...
Barnard +22 more
core +1 more source
Evolving Lac repressor for enhanced inducibility [PDF]
Protein Engineering, Design and Selection, 2008 Lactose repressor (LacI) is one of the best studied prokaryotic transcriptional regulatory proteins till date. Detailed structural, biochemical and genetic studies are being carried out on LacI since four decades to understand its ligand binding properties and the basis of allosteric response.
O, Satya Lakshmi, N M, Rao
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Virology Journal, 2005 Background Replication of the vaccinia virus genome occurs in cytoplasmic factory areas and is dependent on the virus-encoded DNA polymerase and at least four additional viral proteins.
Moss Bernard, De Silva Frank S
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Quantitative Characterization of Combinatorial Transcriptional Control of the Lactose Operon of E. coli [PDF]
, 2007 It is the goal of systems biology to understand the behavior of the whole in terms of the knowledge of the parts. This is hard to achieve in many cases due to the difficulty of characterizing the many constituents and their complex web of interactions ...
Hwa, T. +3 more
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Heritable change caused by transient transcription errors. [PDF]
PLoS Genetics, 2013 Transmission of cellular identity relies on the faithful transfer of information from the mother to the daughter cell. This process includes accurate replication of the DNA, but also the correct propagation of regulatory programs responsible for cellular
Alasdair J E Gordon +3 more
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Comptes Rendus. Biologies, 2005 Few proteins have had such a strong impact on a field as the lac repressor has had in Molecular Biology. Over 40 years ago, Jacob and Monod [Genetic regulatory mechanisms in the synthesis of proteins, J. Mol. Biol. 3 (1961) 318] proposed a model for gene regulation, which survives essentially
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