Results 41 to 50 of about 31,663 (206)
Kinetic approaches to lactose operon induction and bimodality
, 2013 The quasi-equilibrium approximation is acceptable when molecular interactions are fast enough compared to circuit dynamics, but is no longer allowed when cellular activities are governed by rare events.
Michel, Denis
core +3 more sources
Lac/Tet Dual-Inducible System Functions in Mammalian Cell Lines
BioTechniques, 1998 The Escherichia coli Lac repressor (Lac system) and tetracycline responsive promoter (Tet system) systems have been used individually to regulate gene expression at the cellular as well as at the organismal levels.
H.-S. Liu +4 more
doaj +1 more source
A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell [PDF]
, 2010 By monitoring fluorescently labeled lactose permease with single-molecule sensitivity, we investigated the molecular mechanism of how an Escherichia coli cell with the lac operon switches from one phenotype to another.
Cai, Long +3 more
core +1 more source
, 2015 Transcriptional repression may cause transcriptional noise by a competition between repressor and RNA polymerase binding. Although promoter activity is often governed by a single limiting step, we argue here that the size of the noise strongly depends on
Mitarai, Namiko +2 more
core +1 more source
Changes in students’ mental models from computational modeling of gene regulatory networks
International Journal of STEM Education, 2019 Background Computational modeling is an increasingly common practice for disciplinary experts and therefore necessitates integration into science curricula. Computational models afford an opportunity for students to investigate the dynamics of biological
Joseph T. Dauer +5 more
doaj +1 more source
Thermodynamic Analysis of Mutant lac Repressors [PDF]
Journal of Molecular Biology, 2011 The lactose (lac) repressor is an allosteric protein that can respond to environmental changes. Mutations introduced into the DNA binding domain and the effector binding pocket affect the repressor's ability to respond to its environment. We have demonstrated how the observed phenotype is a consequence of altering the thermodynamic equilibrium ...
Robert, Daber +2 more
openaire +2 more sources
Physical Constraints and Functional Characteristics of Transcription Factor-DNA Interaction [PDF]
, 2001 We study theoretical ``design principles'' for transcription factor-DNA interaction in bacteria, focusing particularly on the statistical interaction of the transcription factors (TF's) with the genomic background (i.e., the genome without the target ...
Berg +24 more
core +3 more sources
Data in Brief, 2019 The mechanism by which proteins are able to find small cognate sequences in the range from few to few tens of base pairs amongst the millions of non-specific chromosomal DNA has been puzzling researchers for decades.
Carina Monico +4 more
doaj +1 more source
Rules for biological regulation based on error minimization
, 2006 The control of gene expression involves complex mechanisms that show large variation in design. For example, genes can be turned on either by the binding of an activator (positive control) or the unbinding of a repressor (negative control).
Atkinson +37 more
core +2 more sources
Biotechnology and Bioengineering, EarlyView.The probiotic E. coli Nissle 1917 was engineered to enable stable and antibiotic free protein expression platforms using native plasmids. Further modification aids in the production of high‐level protein expression for antimicrobial secretion whose activity is comparable to industry standard E. coli BL21.
Halimatun Sakdiah Zainuddin +2 more
wiley +1 more source