Results 51 to 60 of about 31,663 (206)

Biotechnology by Design: An Introductory Level, Project-Based, Synthetic Biology Laboratory Program for Undergraduate Students

Journal of Microbiology & Biology Education, 2015
Synthetic biology offers an ideal opportunity to promote undergraduate laboratory courses with research-style projects, immersing students in an inquiry-based program that enhances the experience of the scientific process.
Dale L. Beach, Consuelo J. Alvarez
doaj   +1 more source

T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

Microbial Cell Factories, 2022
Background The widespread usage of protein expression systems in Escherichia coli (E. coli) is a workhorse of molecular biology research that has practical applications in biotechnology industry, including the production of pharmaceutical drugs.
Daria Krefft, Maciej Prusinowski, Paulina Maciszka, Aleksandra Skokowska, Joanna Zebrowska, Piotr M. Skowron   +5 more
doaj   +1 more source

Scale‐up of a monoclonal antibody CHO fed‐batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen

Biotechnology Progress, EarlyView.
Abstract Key hydrodynamic‐related parameters such as volumetric power input (P/V), impeller configuration, aeration strategy, and maximum gas sparge rate, as well as an appropriate feeding strategy, must be carefully selected to improve production yields in bioreactor.
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source

Lac repressor - lac operator interaction. Circular dichroism study

Nucleic Acids Research, 1981
The interaction between lac repressor and a small operator DNA fragment have been examined by circular dichroism spectroscopy. The binding of lac repressor on the operator induces a conformation change of the DNA which is different from that observed upon non specific binding on non operator DNA.
F, Culard, J C, Maurizot
openaire   +3 more sources

Expanding the Toolbox for Inducible Protein Expression With Automation‐enabled Generation of Glycomimetics

Chemistry – A European Journal, EarlyView.
Inducible protein expression is a cornerstone of many aspects of industrial and molecular biotechnological processes. 2′‐fucosyl isopropyl‐β‐D‐thiogalactopyranose (2′F‐IPTG) and 2′‐fucosyl isobutyl‐C‐galactoside (2′F‐IBCG) mimics were explored for protein expression studies.
Ashley E. DeYong, Keevan C. Marion, Murat Ozturk, Sanjeeva Kumar Murali, Fatima Enam, Thomas J. Mansell, Nicola L. B. Pohl   +6 more
wiley   +1 more source

Exploiting a Phage-Bacterium Interaction System as a Molecular Switch to Decipher Macromolecular Interactions in the Living Cell

Viruses, 2018
Pathogenicity islands of Staphylococcus aureus are under the strong control of helper phages, where regulation is communicated at the gene expression level via a family of specific repressor proteins.
Éva Viola Surányi, Rita Hírmondó, Kinga Nyíri, Szilvia Tarjányi, Bianka Kőhegyi, Judit Tóth, Beáta G. Vértessy   +6 more
doaj   +1 more source

Epigenetic mechanisms and therapeutic innovations in chronic pain‐associated neuropsychiatric co‐morbidities

British Journal of Pharmacology, EarlyView.
Abstract Chronic pain, marked by nociceptive sensitization and maladaptive neuroplasticity, affects 30% of the global population with escalating socioeconomic burdens. Epidemiological data show a 2‐3‐fold increase in neuropsychiatric co‐morbidities among individuals with chronic pain, where epigenetic dysregulation serves as a key mechanism linking ...
Kai Zhang, Sijia Zhu, Na Xing, Zhen Zhou, Jiayi Chen, Tianen Si, Mingrui Li, Lin Jia, Yousef Rastegar‐Kashkooli, Tom J. Wang, Wei Zhu, Jing Li, Chao Jiang, Junmin Wang, Moxin Wu, Xiaoping Yin, Weidong Zang, Jian Wang, Songxue Su   +18 more
wiley   +1 more source

Nuclear domain 10-associated proteins recognize and segregate intranuclear DNA/protein complexes to negate gene expression

Virology Journal, 2012
Background DNA viruses, such as herpes simplex virus type 1 (HSV-1), Simian virus 40 (SV40), and Cytomegaloviruses (CMV), start their replicative processes and transcription at specific nuclear domains known as ND10 (nuclear domain 10, also called PML ...
Rivera-Molina Yisel A, Rojas Bruno R, Tang Qiyi   +2 more
doaj   +1 more source

Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. [PDF]

PLoS ONE, 2017
The development of plasmid-mediated gene expression control in bacteria revolutionized the field of bacteriology. Many of these expression control systems rely on the addition of small molecules, generally metabolites or non-metabolized analogs thereof ...
Michael J Gebhardt, Rachael K Jacobson, Howard A Shuman   +2 more
doaj   +1 more source

Synthetic biology—putting engineering into biology [PDF]

, 2006
Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Heinemann, Matthias,, Panke, Sven,
core   +1 more source