Results 71 to 80 of about 31,663 (206)

The genotype‐phenotype landscape of an allosteric protein

Molecular Systems Biology, 2021
Allostery is a fundamental biophysical mechanism that underlies cellular sensing, signaling, and metabolism. Yet a quantitative understanding of allosteric genotype‐phenotype relationships remains elusive.
Drew S Tack, Peter D Tonner, Abe Pressman, Nathan D Olson, Sasha F Levy, Eugenia F Romantseva, Nina Alperovich, Olga Vasilyeva, David Ross   +8 more
doaj   +1 more source

Hydrogen Evolution via Oxygen Tolerant [NiFe]‐Hydrogenase Immobilized on TiO2 Nanotubes

Nano Select, Volume 7, Issue 1, January 2026.
Hydrogenase‐immobilized TiO2 nanotube was developed by the electropolymerization of polypyrrole and hydrogenase drop‐casting, and cathodic current is reported. Hydrogenase was extracted from E. coli, which can be more oxygen tolerant than the previous hydrogenase electrode.
Hwapyong Kim, Ki Nam Kim, Sung Hyun Lee, Chang‐Hoon Nam, Young‐Sam Lee, Su‐Il In   +5 more
wiley   +1 more source

Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining

BMC Biotechnology, 2007
Background GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybridization (FISH).
Rohr Karl, Eils Roland, Otten Simone, Knerr Boris, Nagel Jens, Kim Il-Han, Dietzel Steffen   +6 more
doaj   +1 more source

Stochastic dynamics of macromolecular-assembly networks [PDF]

, 2006
The formation and regulation of macromolecular complexes provides the backbone of most cellular processes, including gene regulation and signal transduction.
Arkin A, Hill TL, Jose MG Vilar, Leonor Saiz, Ptashne M   +4 more
core   +3 more sources

Laccases from lactic acid bacteria show cuprous oxidase activity and capture Cu(II) and Ag(I) ions

Protein Science, Volume 35, Issue 1, January 2026.
Abstract Several laccases derived from lactic acid bacteria (LAB) display specific structural features, such as two methionine residues at the entrance of the T1Cu center, and an extended C‐terminal end enriched in methionine and histidine. To investigate their functional roles, we engineered mutant variants of the laccase Pp4816 from Pediococcus ...
Raquel Gascó, Ramon Sendra, Isidoro Olmeda, Francisco Paredes‐Martínez, Sergi Ferrer, Isabel Pardo, Patricia Casino   +6 more
wiley   +1 more source

Designer Gene Networks: Towards Fundamental Cellular Control

, 2001
The engineered control of cellular function through the design of synthetic genetic networks is becoming plausible. Here we show how a naturally occurring network can be used as a parts list for artificial network design, and how model formulation leads ...
Ackers, Arkin, Barkai, Becksei, Bialek, Bray, Chen, Dickson, Elowitz, Endy, Gardner, Gillespie, Glass, Glass, Glass, Hammond, Harada, Harding, Hartwell, Hasty, Jacob, Johnson, Johnson, Kauffman, Keller, Lauffenburger, Lowman, McAdams, McAdams, McAdams, McClure, Meyer, Monod, Monod, Moran, Novak, Novak, Novick, Ohlendorf, Palsson, Ptaschne, Reinitz, Rozanov, Samoilov, Sancho, Savageau, Savageau, Savageau, Shea, Smolen, Sveiczer, Szafranski, Tyson, Villaverde, von Hippel, Wolf, Wong   +56 more
core   +1 more source

DNA looping: the consequences and its control [PDF]

, 2006
The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication.
Ackers, Adhya, Alberts, Becker, Bloomfield, Broach, Cloutier, de Lange, Dodd, Du, Dunn, Halford, Hanke, Hill, Irani, Jose MG Vilar, Knight, Leonor Saiz, Lewis, Li, Matthews, Moreau, Mossing, Muller, Müller-Hill, Oehler, Olson, Ptashne, Ptashne, Radman-Livaja, Revet, Richmond, Ringrose, Rippe, Saiz, Saiz, Schleif, Semsey, Stenger, Tolhuis, Vilar, Vilar, Villa, Yan, Yasmin, Zhang   +45 more
core   +1 more source

Interaction of Staphylococcus aureus di‐iron Repair of Iron Centers protein with the iron–sulfur cluster assembly SUF system

Protein Science, Volume 35, Issue 1, January 2026.
Abstract Repair of Iron Centers (RIC) proteins are present in several pathogens, including Staphylococcus aureus, contributing to resistance to nitroxidative stress and virulence. In this work, we investigated the molecular mechanisms by which S. aureus RIC facilitates iron mobilization, through structural analyses and functional studies.
Frederico M. Lourenço, Liliana S. O. Silva, Mafalda R. Almeida, Filipe Folgosa, Pedro M. Matias, Sandra M. Carvalho, João C. Lima, Célia V. Romão, Lígia M. Saraiva   +8 more
wiley   +1 more source

Evolutionary potential of a duplicated repressor-operator pair: simulating pathways using mutation data. [PDF]

PLoS Computational Biology, 2006
Ample evidence has accumulated for the evolutionary importance of duplication events. However, little is known about the ensuing step-by-step divergence process and the selective conditions that allow it to progress. Here we present a computational study
Frank J Poelwijk, Daniel J Kiviet, Sander J Tans   +2 more
doaj   +1 more source

Rapid Identification of Superior Endogenous Signal Peptides for Heterologous Protein Secretion by Corynebacterium glutamicum Through Modular Cloning and Automation

Microbial Biotechnology, Volume 19, Issue 1, January 2026.
A modular cloning approach combined with an automated high‐throughput platform enabled rapid identification of superior endogenous signal peptides for the secretion of proteins of interest (POIs), such as industrial enzymes, by Corynebacterium glutamicum. This streamlined workflow accelerates discovery and development of efficient bacterial systems for
Susana Matamouros, Julia Tenhaef, Astrid Bida, Stephan Noack, Michael Bott   +4 more
wiley   +1 more source