Results 71 to 80 of about 1,710,497 (241)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Palynological Evidence of Late Holocene Paleo‐Monsoon in Eastern Pamir

Geophysical Research Letters, 2019
The Tibetan Plateau, which is climatically dominated by the westerlies and the Asian monsoon, has a major influence on the atmospheric circulation and water resources of Asia.
Keliang Zhao, Xinying Zhou, Ming Ji, Xiaoqiang Li   +3 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Holocene warm-humidification trends recorded in lacustrine sediments in Arid Central Asia: Synthesis insights from climate reconstruction, carbonate carbon-oxygen isotopes and n-alkanes

Journal of Hydrology: Regional Studies
Study region: Arid Central Asia (ACA). Study focus: The contradictory understanding of Holocene climate change trends in ACA remains unresolved. This study compiled Holocene temperature and moisture changes from 23 lacustrine sediment sites in the ACA ...
Xiaojun Wang, Qianqian Yang, Hantao Ni, Chunxia Zhang   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

LATE GLACIAL AND HOLOCENE EROSIONAL DISSECTION AND FILL IN THE TEMPORAL (EPHEMERAL) STREAM VALLEY ON THE RIGHT BANK OF THE SELENGA RIVER

Геодинамика и тектонофизика
The structure, composition and cut and fill sequence of the Late Glacial and Holocene erosional landforms in the Kuitunka River basin on the right bank of the Selenga River were studied based on a detailed analysis and radiocarbon dating of the soil ...
Yu. V. Ryzhov, V. A. Golubtsov, M. V. Smirnov   +2 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Late Pleistocene to Holocene Depositional Environments in Foredeep Basins: Coastal Plain Responses to Sea-Level and Tectonic Forcing—The Metaponto Area (Southern Italy)

Geosciences
The Metaponto coastal plain (Ionian margin, Southern Italy) records the Late Pleistocene–Holocene evolution of a foredeep coastal system shaped by relative sea-level change, vertical land motion, and compaction. We analyze a 22 m continuous core (Meta 1)
Agostino Meo, Maria Rosaria Senatore
doaj   +1 more source