Results 41 to 50 of about 102,661 (259)

Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein

FEBS Letters, EarlyView.
Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux, Christian Lévêque, Fodil Azzaz, Marion Sangiardi, Sarosh R Irani, Michael Seagar, Jacques Fantini, Oussama El Far   +7 more
wiley   +1 more source

Preliminary clinical performance of a Cas13a-based lateral flow assay for detecting Neisseria gonorrhoeae in urine specimens

mSphere
Nucleic acid amplification testing (NAAT) for N. gonorrhoeae is unavailable in resource-limited settings. We previously developed a CRISPR-based lateral flow assay for detecting N. gonorrhoeae.
Lao-Tzu Allan-Blitz, Gordon Adams, Gabriela Sanders, Palak Shah, Krithik Ramesh, Jana Jarolimova, Kevin L. Ard, John A. Branda, Jeffrey D. Klausner, Pardis C. Sabeti, Jacob E. Lemieux   +10 more
doaj   +1 more source

A point-of-care lateral flow assay for neutralising antibodies against SARS-CoV-2

EBioMedicine, 2021
Background: As vaccines against SARS-CoV-2 are now being rolled out, a better understanding of immunity to the virus, whether from infection, or passive or active immunisation, and the durability of this protection is required. This will benefit from the
Thomas S. Fulford, Huy Van, Nicholas A. Gherardin, Shuning Zheng, Marcin Ciula, Heidi E. Drummer, Samuel Redmond, Hyon-Xhi Tan, Irene Boo, Rob J. Center, Fan Li, Samantha L. Grimley, Bruce D. Wines, Thi H.O. Nguyen, Francesca L. Mordant, Paula Ellenberg, Louise C. Rowntree, Lukasz Kedzierski, Allen C. Cheng, Denise L. Doolan, Gail Matthews, Katherine Bond, P. Mark Hogarth, Zoe McQuilten, Kanta Subbarao, Katherine Kedzierska, Jennifer A. Juno, Adam K. Wheatley, Stephen J. Kent, Deborah A. Williamson, Damian F.J. Purcell, David A. Anderson, Dale I. Godfrey   +32 more
doaj   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Clinical Performance of Lateral Flow Assay for Cryptosporidium spp. Diagnosis

Biomedicines, 2023
Cryptosporidium spp. is an apicomplexan protozoan parasite associated with gastroenteritis in humans. In 2018, Spain showed 1511 confirmed cases, with a growing trend since 2014. Despite this fact, Cryptosporidium spp.
Miriam Campos-Ruiz, Clara Flamarich, Anabel Fernández-Navarro, Silvia Roure, Laura Martin, Pablo Pillado, Pere-Joan Cardona, Gema Fernández-Rivas   +7 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Lateral flow immunoassay for rapid detection of carbapenemase-producing E. coli and K. pneumoniae at Cho Ray Hospital, Vietnam

Journal of Infection in Developing Countries
Introduction: Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae represent an escalating threat to global public health, particularly in Southeast Asia, where antimicrobial resistance (AMR) is highly prevalent.
Ngoc B Tran, Phu T Truong, Van TH Tran, Dat Q Ngo   +3 more
doaj   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

Biosensors, 2012
Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection ...
Sam R. Nugen, Catherine Fill, Yuhong Wang   +2 more
doaj   +1 more source