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Aptamer-based lateral flow assay on-site biosensors
Biosensors and Bioelectronics, 2021The lateral flow assay (LFA) is a widely used paper-based on-site biosensor that can detect target analytes and obtain test results in several minutes. Generally, antibodies are utilized as the biorecognition molecules in the LFA. However, antibodies selected using an in vivo process not only may risk killing the animal hosts and causing errors between
Lei Huang +5 more
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Analytical Biochemistry, 2021
MicroRNAs (miRNAs) are short RNA sequences found in eukaryotic cells and they are involved in several diseases pathogenesis including different types of cancers, metabolic and cardiovascular disorders. Thus, miRNAs circulating in serum, plasma, and other body fluids are employed as biomarkers for diagnostic and prognostic purposes and in assessment of ...
Niloofar, Khandan-Nasab +5 more
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MicroRNAs (miRNAs) are short RNA sequences found in eukaryotic cells and they are involved in several diseases pathogenesis including different types of cancers, metabolic and cardiovascular disorders. Thus, miRNAs circulating in serum, plasma, and other body fluids are employed as biomarkers for diagnostic and prognostic purposes and in assessment of ...
Niloofar, Khandan-Nasab +5 more
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An enzyme-amplified lateral flow strip biosensor for visual detection of MicroRNA-224
Talanta, 2016An enzyme-based dual-labeled nanoprobe is designed to fabricate a sensitive enzyme-amplified lateral flow biosensor for visual detection of mircoRNA-224 (miRNA-224). The recognition DNA probe (detection probe) and signal amplification enzyme (Horseradish peroxidase, HRP) are immobilized on gold nanoparticle (GNPs) surface, simultaneously.
Xuefei Gao, Li-Ping Xu, Yongqiang Wen
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A lateral flow biosensor for rapid detection of DNA-binding protein c-jun
Biosensors and Bioelectronics, 2011A lateral flow biosensor based on an immuno-chromatographic assay has been developed for the detection of DNA-binding proteins. The biosensor is composed of four parts: a sample pad, a conjugate pad, a strip of nitrocellulose membrane and an absorbent pad.
Zhiyuan Fang +2 more
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2016
A typical lateral flow biosensor employs a sandwich detection system and uses tracer(s) to generate detectable signal(s) (Figure 13.1). However, recent advances in nanomaterials offer a wide variety of improvement options, including new detection methodologies, signal transformation and improvements in sensitivity and specificity [4-6]. In this chapter,
Li, Jia, Macdonald, J
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A typical lateral flow biosensor employs a sandwich detection system and uses tracer(s) to generate detectable signal(s) (Figure 13.1). However, recent advances in nanomaterials offer a wide variety of improvement options, including new detection methodologies, signal transformation and improvements in sensitivity and specificity [4-6]. In this chapter,
Li, Jia, Macdonald, J
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Gold nanorods-based lateral flow biosensors for sensitive detection of nucleic acids
Microchimica Acta, 2021A gold nanorod (AuNR)-based lateral flow nucleic acid biosensor (LFNAB) is reported for visual detection of DNA with a short test time and high sensitivity. AuNRs with an approximate length of 60 nm were utilized as a colored tag to label the detection DNA probe (Det-DNA).
Qingcai Yu +6 more
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Lateral Flow Biosensors for the Detection of Nucleic Acid
2013The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are time-consuming and labor-intensive. Recently, isothermal strand-displacement reaction-based lateral flow biosensors have attracted a great deal of ...
Lingwen, Zeng +3 more
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A lateral flow biosensor for the detection of human pluripotent stem cells
Analytical Biochemistry, 2013A lateral flow biosensor based on immunoassay has been developed for the detection of human stem cells for the first time. Antibody specific for a human stem cell surface antigen, SSEA-4, is coated onto gold nanoparticles, whereas antibody against another human pluripotent stem cell surface antigen, SSEA-3, is immobilized on the test zone of the NC ...
Wei, Wu +4 more
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Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2020
Vaspin is a protein present in human serum that can cause type-2 diabetes, obesity, and other cardiovascular diseases. We report fluorescent upconverting nanoparticles (UCNPs)-based lateral flow biosensor for ultrasensitive detection of Vaspin. A pair (primary and secondary) of cognate aptamers was used that has duo binding with Vaspin.
Muhsin Ali +2 more
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Vaspin is a protein present in human serum that can cause type-2 diabetes, obesity, and other cardiovascular diseases. We report fluorescent upconverting nanoparticles (UCNPs)-based lateral flow biosensor for ultrasensitive detection of Vaspin. A pair (primary and secondary) of cognate aptamers was used that has duo binding with Vaspin.
Muhsin Ali +2 more
exaly +3 more sources
IEEE Transactions on NanoBioscience, 2023
Methamphetamine is a powerful stimulant drug, the abuse of which threatens human health and social stability. Rapid and accurate quantification of methamphetamine is essential to inhibit the abuse and prevalence of methamphetamine effectively. In this paper, we present a portable fluorescence reader with upconverting nanoparticle-labeled lateral flow ...
Lei Huang +5 more
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Methamphetamine is a powerful stimulant drug, the abuse of which threatens human health and social stability. Rapid and accurate quantification of methamphetamine is essential to inhibit the abuse and prevalence of methamphetamine effectively. In this paper, we present a portable fluorescence reader with upconverting nanoparticle-labeled lateral flow ...
Lei Huang +5 more
openaire +2 more sources