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Ultrasensitive nucleic acid biosensor based on enzyme–gold nanoparticle dual label and lateral flow strip biosensor

Biosensors and Bioelectronics, 2011
In this article, we describe an ultrasensitive nucleic acid biosensor (NAB) based on horseradish peroxidase (HRP)-gold nanoparticle (Au-NP) dual labels and lateral flow strip biosensor (LFSB). The results presented here expand on prior work (Mao et al., 2009a) by optimizing the preparation of HRP-Au-NP-DNA conjugates.
Xibao Zhang   +2 more
exaly   +3 more sources

Paper-Based Biosensors with Lateral/Vertical Flow Assay

2020
The lateral flow assay (LFA), which is a simple paper-based device for the detection and analysis of disease, is now the most predominant diagnostic platform in point-of-care (POC) markets [1, 2, 3, 4]. This market-dominating power stems from the unique characteristics of the LFA: low cost, portability, versatility, easy use, free of other instruments,
Dohwan Lee, Jeong Hoon Lee
openaire   +1 more source

A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms

2017
A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase ...
Lingwen, Zeng, Zhuo, Xiao
openaire   +2 more sources

Nanolabels for biosensors based on lateral flow immunoassays

Analytica Chimica Acta
The COVID-19 outbreak was an important turning point in the development of a new generation of biosensing technologies. The synergistic combination of an immunochromatographic test (lateral flow immunoassays, LFIA) and signal transducers provides enhanced sensitivity and the ability to quantify in the rapid tests. This is possible due to the variety of
C. Saweres-Argüelles   +4 more
openaire   +2 more sources

A lateral flow biosensor for detection of nucleic acids with high sensitivity and selectivity

Chem. Commun., 2012
A lateral flow biosensor based on isothermal strand-displacement polymerase reaction and gold nanoparticles has been developed for the visual detection of nucleic acids with a detection limit of 0.01 fM.
Puchang, Lie   +4 more
openaire   +2 more sources

A lateral flow biosensor for rapid detection of Singapore grouper iridovirus (SGIV)

Aquaculture, 2021
Abstract Iridoviruses are well-known as causative agents of serious systemic diseases, and have been identified in more than 100 fish species worldwide in the last few decades. Singapore grouper iridovirus (SGIV) is a devastating viral pathogen of grouper aquaculture, and has caused substantial economic losses in China and Southeast Asia.
Jiaxin Liu   +8 more
openaire   +1 more source

Lateral Flow Biosensors Based on Gold Nanoparticles

2014
Abstract The aim of these authors is to offer to the reader the general basis of lateral flow technology and overall of that based on the use of gold nanoparticles, its design, and various useful analytical applications. The historical progress of this technology discussed first is followed by a detailed description of the basic principles, formats ...
Lourdes Rivas   +3 more
openaire   +1 more source

A Variety of Bio-nanogold in the Fabrication of Lateral Flow Biosensors for the Detection of Pathogenic Bacteria

Current Topics in Medicinal Chemistry, 2019
Pathogenic bacteria constitute one of the most serious threats to human health. This has led to the development of technologies for the rapid detection of bacteria. Bio-nanogold-based lateral flow biosensors (LFBs) are a promising assay due to their low limit of detection, high sensitivity, good selectivity, robustness, low cost, and quick assay ...
Nan, Cheng   +5 more
openaire   +2 more sources

Carbon nanotube-based lateral flow biosensor for sensitive and rapid detection of DNA sequence

Biosensors and Bioelectronics, 2015
In this article, we describe a carbon nanotube (CNT)-based lateral flow biosensor (LFB) for rapid and sensitive detection of DNA sequence. Amine-modified DNA detection probe was covalently immobilized on the shortened multi-walled carbon nanotubes (MWCNTs) via diimide-activated amidation between the carboxyl groups on the CNT surface and amine groups ...
Wanwei Qiu   +2 more
exaly   +3 more sources

Biosensors and lateral flow immunoassays: Current state and future prospects

Clinica Chimica Acta
The advent of paper-based biosensors represents a novel paradigm in point-of-care (POC) diagnostics, emerging as versatile tools. However, the broad term "biosensors" can be misleading, encompassing a range of techniques such as dipstick assays, electrochemical, microfluidics and immunoassay-based biosensors (including lateral flow (LFA), vertical flow
Raveena, Udhani   +2 more
openaire   +2 more sources

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