Results 121 to 130 of about 2,915 (158)

Rapid authentication of Cordyceps by lateral flow dipstick

Journal of Pharmaceutical and Biomedical Analysis, 2015
Cordyceps (Dongchongxiacao), a valuable traditional Chinese medicine, is composed of the fruiting body of Ophiocordyceps sinensis (Family: Ophiocordycipitaceae) on a caterpillar of ghost-moth species (Family: Hepialidae). Owing to its multiple potential functions, Cordyceps are in great demand and represent significant economic value.
Yuk-Lau, Wong, Ka-Lok, Wong, Pang-Chui, Shaw   +2 more
openaire   +2 more sources

Development of a lateral flow dipstick immunoassay for evaluation of folate levels in maize

Analytical and Bioanalytical Chemistry, 2017
Folates (vitamin B9) are essential for all organisms as cofactors for one-carbon metabolism. However, measurement of folates is technically complicated and time-consuming. In this study, we developed a dipstick immunoassay using a folate-specific monoclonal antibody (mAb), allowing rapid and low-cost detection of folates.
Qiuju, Liang, Chen, Yi, Ling, Jiang, Guiyu, Tan, Chunyi, Zhang, Baomin, Wang   +5 more
openaire   +2 more sources

Detection of Prorocentrum minimum by hyperbranched rolling circle amplification coupled with lateral flow dipstick

Environmental Science and Pollution Research, 2020
A novel method referred to as hyperbranched rolling circle amplification (HRCA) coupled with lateral flow dipstick (LFD) (HRCA-LFD) here was developed for specific, sensitive, rapid, and simple detection of Prorocentrum minimum. HRCA-LFD relies on a padlock probe (PLP) consisting of a common ligation sequence, two terminal sequences that complement the
Fuguo, Liu, Chunyun, Zhang, Yuchen, Yang, Yudan, Yang, Yuanyuan, Wang, Guofu, Chen   +5 more
openaire   +2 more sources

A visual method to detect meat adulteration by recombinase polymerase amplification combined with lateral flow dipstick

Food Chemistry, 2021
Determining the animal source in meat and meat products is crucial to prevent meat adulteration and fraud. Conventional methods require considerable operator skills, expensive instruments and are unable to provide fast mobile on-site detection systems to detect contamination of meat products. We developed a visual method based on recombinase polymerase
Liyun, Lin, Yuzhong, Zheng, Huiying, Huang, Fenluan, Zhuang, Huixia, Chen, Guangcai, Zha, Peikui, Yang, Zhonghe, Wang, Meilan, Kong, Huagui, Wei, Xianghui, Zou, Min, Lin   +11 more
openaire   +2 more sources

Establishment of the Recombinase Polymerase Amplification–Lateral Flow Dipstick Detection Technique for Fusarium oxysporum

Plant Disease, 2023
Fusarium oxysporum causes crown rot, wilt, root rot, and many other major plant diseases worldwide. During the progression of strawberry crown rot disease, the pathogen is transmitted from the mother plant to the seedling through the stolon, with obvious characteristics of latent infection. Therefore, rapid and timely detection of F.
Shuodan Hu, Chenyi Yan, Hong Yu, Yu Zhang, Chuan-Qing Zhang   +4 more
openaire   +2 more sources

Visual detection of Mycoplasma pneumoniae by the recombinase polymerase amplification assay coupled with lateral flow dipstick

Journal of Microbiological Methods, 2022
Mycoplasma pneumoniae (Mp) is a common cause of respiratory infections in school-age children. In the present study, a recombinase polymerase amplification (RPA) assay combined with lateral flow dipstick (LFD) was developed for diagnosis of Mp. Specific primers and a probe were designed for the Mp p1 gene. The optimal reaction conditions of the RPA-LFD
Mengjie, Zhu, Lei, Ma, Qingfeng, Meng, Zhuangbo, Guo   +3 more
openaire   +2 more sources

A Simple and Sensitive “Dipstick” Test in Serum Based on Lateral Flow Separation of Aptamer‐Linked Nanostructures

Angewandte Chemie International Edition, 2006
Aptamers are single-stranded nucleic acids that can be selected from a large random library to bind a number of molecules with high affinity and specificity. Therefore, they are considered to be the nucleic acid version of antibodies. Since its first discovery in the early 1990s, the use of aptamers for sensing and diagnostic applications has been ...
Juewen, Liu, Debapriya, Mazumdar, Yi, Lu
openaire   +2 more sources

Visualized Detection of Tobacco Anthracnose by a Recombinase Polymerase Amplification–Lateral Flow Dipstick Assay

Plant Disease
Anthracnose caused by Colletotrichum spp. is a widespread fungal disease that is detrimental to tobacco growth and inflicts economic damage of up to 100 million in tobacco-growing regions in China. An early diagnostic tool is vital for the accurate determination and management of anthracnose in the field.
Tao Li, Ji Feng, Yangyin Chen, Yu Zhang, Hancheng Wang, Chuanqing Zhang   +5 more
openaire   +2 more sources

[Development of a lateral flow dipstick immunoassay for rapid detection of ginsenoside Re].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2014
A sensitive antibody-based lateral flow dipstick was developed for ginsenoside Re (GRe) detection. The stick consisted of a sample pad, a conjugate pad, membrane and an absorbent pad. The membrane was coated with two capture reagents, GRe-BSA conjugate and goat anti-mouse antibodies, forming a test line and a control line, respectively.
Tie-Gui, Nan, Zhen, Cao, Li-Shan, He, Yuan, Yuan, Lu-Qi, Huang, Bao-Min, Wang   +5 more
openaire   +1 more source

The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Karlodinium veneficum

Harmful Algae, 2017
The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) combined with a chromatographic lateral flow dipstick (LFD) assay to rapidly and specifically detect the Karlodinium veneficum ITS gene. Four groups of LAMP primers were specially designed to target the K. veneficum ITS gene.
Hai-Long, Huang, Peng, Zhu, Cheng-Xu, Zhou, Shan, He, Xiao-Jun, Yan   +4 more
openaire   +2 more sources