Results 181 to 190 of about 14,552 (222)
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Subcellular localisation of purinemetabolising enzymes in Leishmania mexicana mexicana
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1985Leishmania mexicana mexicana cultured promastigotes were fractionated by isopycnic centrifugation on linear sucrose gradients. Guanine, hypoxanthine and xanthine phosphoribosyltransferase activities were found to be associated with glycosomes, whereas adenine phosphoribosyltransferase was cytosolic.
H F, Hassan, J C, Mottram, G H, Coombs
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Respiration of Leishmania mexicana amastigotes and promastigotes
Molecular and Biochemical Parasitology, 1981Promastigotes of Leishmania mexicana mexicana recently derived from amastigotes by transformation in vitro respired at a rate (17 nmol O2/min per 10(8) parasites) 4-5 times higher than that of amastigotes, but when the difference in cell protein content between the two preparations was taken into account the rates were not significantly different (32 ...
D T, Hart, K, Vickerman, G H, Coombs
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Heme binding to Leishmania mexicana amazonensis
Molecular and Biochemical Parasitology, 1988Leishmania mexicana amazonensis is a pathogenic parasite whose growth, due to a biosynthetic deficiency, is dependent on a supply of exogenous heme. Utilizing [55Fe]hemin, we have demonstrated that heme binding to non-dividing cultured promastigotes of L. m.
R A, Galbraith, M J, McElrath
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Pentose phosphate metabolism in Leishmania mexicana
Molecular and Biochemical Parasitology, 2003The metabolism of pentose phosphates was studied in Leishmania mexicana promastigotes. Each of the enzymes of the classical pentose phosphate pathway (PPP) has been identified and specific activities measured. Functioning of the PPP was demonstrated in non-growing cells by measuring the evolution of 14CO2 from [1-14C]D-glucose and [6-14C]D-glucose ...
Dante Abel, Maugeri +4 more
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Chromosome rearrangement in Leishmania mexicana M379
Molecular and Biochemical Parasitology, 1991Circular extrachromosomal elements were observed in a variety of Leishmania species. We show here that two lines originating from the same isolate have been found to contain a circular DNA molecule of 26.6 kb and a linear chromosome of about 250 kb, respectively, which share a homology of more than 20 kb.
Liu, J. +9 more
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Amine production byLeishmania mexicana
Annals of Tropical Medicine & Parasitology, 1985Growing promastigotes of Leishmania mexicana mexicana excreted large amounts of urea and ammonia into the culture medium. Both promastigotes and amastigotes in short-term, high-density cultures also produced urea and ammonia; the excretion rate was higher in promastigotes.
G H, Coombs, B E, Sanderson
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Leishmania mexicana mexicana gp63 is a site-specific neutral endopeptidase
Molecular and Biochemical Parasitology, 1990Leishmania mexicana, like other species of the genus, has a major 63-kDa surface glycoprotein (gp63) that is an active protease. Reports differ as to whether gp63 is a neutral or an acidic protease. Using three radiolabeled synthetic peptide substrates, gp63 purified from L. m.
H S, Ip +3 more
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Molecular and Biochemical Parasitology, 1994
The glycolytic enzyme glucosephosphate isomerase (PGI) is present in two different cell compartments of Leishmania mexicana promastigotes; more than 90% of the activity was detected in the cytosol, the remainder in glycosomes. This subcellular distribution contrasts with that in Trypanosoma brucei, in which the enzyme activity has been mainly located ...
K, Nyame +3 more
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The glycolytic enzyme glucosephosphate isomerase (PGI) is present in two different cell compartments of Leishmania mexicana promastigotes; more than 90% of the activity was detected in the cytosol, the remainder in glycosomes. This subcellular distribution contrasts with that in Trypanosoma brucei, in which the enzyme activity has been mainly located ...
K, Nyame +3 more
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Purine phosphoribosyltransferases of Leishmania mexicana mexicana and other flagellate protozoa
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1985Amastigotes and cultured promastigotes of Leishmania mexicana mexicana and L. m. amazonensis, cultured promastigotes of L. donovani and L. tarentolae, and the culture forms of Crithidia fasciculata, Herpetomonas muscarum muscarum and H. m. ingenoplastis all possessed four phosphoribosyltransferase (PRTase) activities: adenine PRTase, hypoxanthine ...
H F, Hassan, G H, Coombs
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Molecular and Biochemical Parasitology, 1990
The multiple cysteine proteinases characteristic of the amastigote forms of Leishmania mexicana mexicana have been shown to be of three types. The groups of enzymes are distinguished by their substrate specificities and physical properties and have been purified free from other proteinases and most other proteins.
C D, Robertson, G H, Coombs
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The multiple cysteine proteinases characteristic of the amastigote forms of Leishmania mexicana mexicana have been shown to be of three types. The groups of enzymes are distinguished by their substrate specificities and physical properties and have been purified free from other proteinases and most other proteins.
C D, Robertson, G H, Coombs
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