Results 221 to 230 of about 15,700 (261)
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Glucose transport in amastigotes and promastigotes of Leishmania mexicana mexicana
Molecular and Biochemical Parasitology, 1995Promastigotes and amastigotes of Leishmania mexicana mexicana transported 2-deoxy-D-glucose (2-DOG) by a saturable process with a Km of 24 +/- 3 microM and Vmax of 2.21 nmol min-1 (mg protein)-1 for the promastigote and a Km of 29 +/- 8 microM and Vmax of 0.13 nmol min-1 (mg protein)-1 for the amastigote stage.
R J, Burchmore, D T, Hart
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Inducible protein stabilization system in Leishmania mexicana
Molecular and Biochemical Parasitology, 2017Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types.
Lucie, Podešvová +2 more
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Leishmania mexicana: promastigotes migrate through osmotic gradients
Experimental Parasitology, 2002During the insect phase of the parasite lifecycle, Leishmania promastigotes move from the midgut to the anterior regions of the alimentary tract of their sandfly vector. Chemotaxis of Leishmania promastigotes towards sugars has been reported, and the putative presence of sugar gradient in the insect foregut has been suggested to play a role in ...
Graham, Leslie +2 more
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Respiration of Leishmania mexicana amastigotes and promastigotes
Molecular and Biochemical Parasitology, 1981Promastigotes of Leishmania mexicana mexicana recently derived from amastigotes by transformation in vitro respired at a rate (17 nmol O2/min per 10(8) parasites) 4-5 times higher than that of amastigotes, but when the difference in cell protein content between the two preparations was taken into account the rates were not significantly different (32 ...
D T, Hart, K, Vickerman, G H, Coombs
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Leishmania mexicana: Amastigote hydrolases in unusual lysosomes
Experimental Parasitology, 1986Leishmania mexicana mexicana (M379) amastigotes were found to contain much higher activities than cultured promastigotes of five putative lysosomal enzymes: cysteine proteinase; arylsulfatase (EC 3.1.6.1); beta-glucuronidase (EC 3.2.1.31); DNase (EC 3.1.22.1), and RNase (EC 3.1.27.1).
M F, Pupkis, L, Tetley, G H, Coombs
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Molecular and Biochemical Parasitology, 1994
The glycolytic enzyme glucosephosphate isomerase (PGI) is present in two different cell compartments of Leishmania mexicana promastigotes; more than 90% of the activity was detected in the cytosol, the remainder in glycosomes. This subcellular distribution contrasts with that in Trypanosoma brucei, in which the enzyme activity has been mainly located ...
K, Nyame +3 more
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The glycolytic enzyme glucosephosphate isomerase (PGI) is present in two different cell compartments of Leishmania mexicana promastigotes; more than 90% of the activity was detected in the cytosol, the remainder in glycosomes. This subcellular distribution contrasts with that in Trypanosoma brucei, in which the enzyme activity has been mainly located ...
K, Nyame +3 more
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Experimental Parasitology, 1979
Abstract The effect of certain inoculation conditions on the incubation period and development of cutaneous Leishmania braziliensis and Leishmania mexicana lesions was determined in golden hamsters infected in multiple sites. These conditions included: use of the nose, ear, forepaw, and flank as sites of inoculation; use of the intradermal and ...
H R, Wilson, B S, Dieckmann, G E, Childs
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Abstract The effect of certain inoculation conditions on the incubation period and development of cutaneous Leishmania braziliensis and Leishmania mexicana lesions was determined in golden hamsters infected in multiple sites. These conditions included: use of the nose, ear, forepaw, and flank as sites of inoculation; use of the intradermal and ...
H R, Wilson, B S, Dieckmann, G E, Childs
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Leishmania mexicana and Leishmania tropica: Cross immunity in C57BL/6 mice
Experimental Parasitology, 1979Abstract Leishmania mexicana and Leishmania tropica infection were comparatively studied in C57BL/6 mice. Infection with 10 4 amastigotes of L. mexicana was followed by the appearance of a single lesion which ulcerated in 8 weeks and healed in 24 weeks. Mice infected with 10 4 amastigotes of L.
H, Pérez, B, Arredondo, R, Machado
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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
The particulate activities of Leishmania mexicana mexicana amastigote malate dehydrogenase (L-malate:NAD+ oxidoreductase, EC 1.1.1.37) and phosphoenolpyruvate carboxykinase (ATP:oxaloacetate carboxy-lyase (transphosphorylating) EC 4.1.1.49) have been purified to apparent electrophoretic homogeneity by hydrophobic interaction chromatography using Phenyl-
J C, Mottram, G H, Coombs
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The particulate activities of Leishmania mexicana mexicana amastigote malate dehydrogenase (L-malate:NAD+ oxidoreductase, EC 1.1.1.37) and phosphoenolpyruvate carboxykinase (ATP:oxaloacetate carboxy-lyase (transphosphorylating) EC 4.1.1.49) have been purified to apparent electrophoretic homogeneity by hydrophobic interaction chromatography using Phenyl-
J C, Mottram, G H, Coombs
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Leishmania tropica (Wright 1903) and Leishmania mexicana (Biagi 1953)
1995The genus Leishmania has been the subject of much current taxonomic and clinical research.1 Through the use of DNA probes, ELISA tests, and differential respirometry, new species and subspecies of Leishmania have been identified and partially characterized.2 Although taxonomy has introduced new species of this genus, from the clinical point of view ...
Dickson D. Despommier +2 more
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