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Lentiviral Transduction of Immune Cells
2008Gene transfer into mammalian cells has been of crucial importance for studies determining the role of specific genes in the differentiation and cell fate of various hematopoietic lineages. Until recently, the majority of these studies were performed in transformed cell lines due to difficulties in achieving levels of transfection of greater than 1-3 ...
Louise, Swainson +4 more
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Generation of CAR-T Cells by Lentiviral Transduction
2021CAR-T cell therapy is one of the most successful cell-based therapies. T cells are the most common cells to be genetically modified for cancer therapy, not only because T cells have cytotoxicity but also because they are easily cultured ex vivo and genetically modified with viral vectors.
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Transduction of human islets with the lentiviral vector
Transplantation Proceedings, 2004Establishment of an efficient gene delivery system for human pancreatic beta cells is important for the development of diabetes-targeted cell therapies. The human immunodeficiency virus type 1 (HIV-1)-derived lentiviral vector is well documented to be an effective gene transfer tool for various types of cells.
N, Kobayashi +8 more
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Lentiviral Transduction of Mammary Epithelial Cells
2022Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including non-dividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100% of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
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Transduction of Human Islets with Pseudotyped Lentiviral Vectors
Human Gene Therapy, 2004Type I diabetes is caused by an autoimmune-mediated elimination of insulin-secreting pancreatic islets. Genetic modification of islets offers a powerful molecular tool for improving our understanding of islet biology. Moreover, efficient genetic engineering of islets could allow for evaluation of new strategies aimed at preventing islet destruction ...
Gary P, Kobinger +7 more
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Lentiviral Transduction of Human Dendritic Cells
2004Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that play a pivotal role in stimulating antigen-specific T cells in vivo. The cardinal properties of DCs are: the ability to take up, process, and present antigens; (2) the ability to migrate through different tissues into lymphoid organs; and (3) the ability to interact with and ...
Roland, Schroers, Si-Yi, Chen
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Stable Expression by Lentiviral Transduction of Cells
2018Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome, the transgene is stably maintained, and long-term producing cells are established. Here,
Natascha, Gödecke +2 more
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Retroviral/Lentiviral Transduction and Transformation Assay
2009Non-random chromosomal translocations can be found in about half of acute leukaemia patients and mostly lead to either over-expression of proto-oncogenes or creation of novel fusion genes. To assess the oncogenic potential and characterize the underlying mechanisms mediated by these candidate oncoproteins, a retroviral transduction/transformation assay
Bernd B, Zeisig, Chi Wai Eric, So
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Lentiviral Transduction of Mammary Epithelial Cells
2015Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including nondividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
Richard, Iggo, Elodie, Richard
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Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia
Animal Reproduction Science, 2015Recent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of transgenic animals.
Hassan, Abbasi +7 more
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