Results 201 to 210 of about 115,363 (220)

Knocking down schistosomes – promise for lentiviral transduction in parasites

Trends in Parasitology, 2015
Underpinned by major advances in our understanding of the genomes of schistosomes, progress in the development of functional genomic tools is providing unique prospects to gain insights into the intricacies of the biology of these blood flukes, their host relationships, and the diseases that they cause.
Jana, Hagen, Jean-Pierre Y, Scheerlinck, Robin B, Gasser   +2 more
openaire   +2 more sources

Lentiviral Transduction of Primary Human Glioblastoma Cultures

2018
This chapter provides detailed step-by-step instructions for the production of lentiviral particles and the transduction of primary human glioblastoma cultures. Lentiviruses stably transduce both dividing and non-dividing cells, such as quiescent cancer stem cell populations.
Joshua D, Frenster, Julio, Inocencio, Dimitris G, Placantonakis   +2 more
openaire   +2 more sources

Lentiviral Transduction for Optimal LSC/HSC Manipulation

2020
Historically, efficient transduction of hematopoietic stem cells (HSC) to study the role of specific genes on HSC function, as well as to broaden the potential of gene therapy for hematopoietic related diseases has relied on our ability to design vectors capable of delivering the gene of interest without affecting HSC function.
openaire   +2 more sources

Lentiviral Gene Transduction of Mouse and Human Stem Cells

2008
This chapter describes the methods we use to transduce mouse and human hematopoietic stem cells (HSCs) and human embryonic stem cells (hESCs). We provide detailed protocols for producing high-titer lentiviral supernatants by transient transfection and for measuring viral titers.
Zhaohui, Ye, Xiaobing, Yu, Linzhao, Cheng   +2 more
openaire   +2 more sources

Lentiviral transduction of microglial cells.

Glia, 2005
Microglial cells are the resident immune cells of the central nervous system. Their function resembles that of tissue macrophages and, as such, they share many properties with both peripheral macrophages and monocytes. One striking similarity is the difficulty with which these cells can be genetically manipulated via transfection or transduction.
Stephanie, Balcaitis, Jonathan R, Weinstein, Sheng, Li, Jeffrey S, Chamberlain, Thomas, Möller   +4 more
openaire   +1 more source

Improving Lentiviral Transduction Efficiency with Microfluidic Systems

Blood, 2015
Abstract Background: Recent clinical trials have demonstrated the efficacy and safety of gene therapy utilizing HIV-derived lentiviral vectors (LVs) for blood disorders. However, the LV requirements and clinical ex vivo cell transduction protocols used in these studies exposes the limitations of the technology and beckons the need for ...
Reginald Tran, David R Myers, Jordan E Shields, Byungwook Ahn, Yongzhi Qiu, Caroline Hansen, Yumiko Sakurai, Robert Moot, H. Trent Spencer, Christopher B Doering, Wilbur A Lam   +10 more
openaire   +1 more source

Lentiviral Vector Production, Titration, and Transduction of Primary Neurons

2013
Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience.
Baojin, Ding, Daniel L, Kilpatrick
openaire   +2 more sources

Efficient lentiviral transduction of liver requires cell cycling in vivo

Nature Genetics, 2000
Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia-based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs.
PARK F, OHASHI K, CHIU W, NALDINI , LUIGI, KAY MA   +4 more
openaire   +3 more sources

Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells

2009
Human fetal mesenchymal stem cells (hfMSC) demonstrate extensive expansion and differentiation capacities and are hence being studied for use in stem cell therapeutics, including gene delivery. With advanced prenatal diagnosis, fetal gene therapy represents an additional avenue for the treatment of inherited deficiencies.
Mark S K, Chong, Jerry, Chan
openaire   +2 more sources

Lentiviral Transduction of Face and Limb Flaps

Plastic and Reconstructive Surgery, 2012
Ex vivo introduction of an immunomodulatory transgene into a face or hand allograft may improve the risk-to-benefit ratio of vascularized composite allografts. Abrogation of the immunogenicity of the skin component of a face or hand allograft may decrease alloreactivity and permit the induction of immunologic tolerance.
Angelo A, Leto Barone, Zhao Y, Zhou, Michael W, Hughes, Ryan, Park, Ruth M, Schulman, Steven, Lee, Evan N, Vidar, Travis L, Shiba, Erin L, Weber, Curtis L, Cetrulo   +9 more
openaire   +2 more sources