Results 71 to 80 of about 1,111,723 (286)

Development of Large-Scale Downstream Processing for Lentiviral Vectors

Molecular Therapy: Methods & Clinical Development, 2020
The interest in lentiviral vectors (LVs) has increased prominently for gene therapy applications, but few have reached the later stages of clinical trials.
Anniina J. Valkama, Igor Oruetxebarria, Eevi M. Lipponen, Hanna M. Leinonen, Piia Käyhty, Heidi Hynynen, Vesa Turkki, Joonas Malinen, Tuukka Miinalainen, Tommi Heikura, Nigel R. Parker, Seppo Ylä-Herttuala, Hanna P. Lesch   +12 more
doaj   +1 more source

PCGF1 Promotes the Tumorigenesis of Malignancies Through Upregulating CCDC34 in Glioma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Polycomb group factor 1 (PCGF1) and coiled‐coil domain‐containing protein 34 (CCDC34) have been implicated in tumorigenesis in multiple malignancies. However, their biological functions and prognostic significance in glioma remain poorly defined.
Yuanbing Chen, Jianbing Xiong, Ziran Ou, Tianhao Zhao, Hui Li, Jun Huang, Zhi Tian, Wuyang Cao   +7 more
wiley   +1 more source

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Production of Lentiviral Vector Expressing MicroRNA-148b

مجله دانشکده پزشکی اصفهان, 2017
Background: Micro (mi)RNAs are non-coding endogenous RNAs which regulate gene expression by hybridization to specific binding sites in target mRNA sequences.
Samaneh Mollazadeh, Vajiheh Neshati, Bibi Sedigheh Fazly Bazzaz, Majid Mojarrad, Mohammad Amin Kerachian   +4 more
doaj  

Advancing the Safety of Lentiviral Vector Mediated Gene Therapy [PDF]

, 2015
Indiana University-Purdue University Indianapolis (IUPUI)Lentiviral vector mediated gene therapy has made great strides in recent years with several successful clinical trials.
Shaw, Aaron Marcus
core   +1 more source

Remote Activation of Wnt Signaling and Cell Proliferation by E‐Cadherin Magnetomechanical Stimulation

Advanced Functional Materials, EarlyView.
A pioneering magnetogenetic toolkit enables non‐invasive, precise spatiotemporal modulation of intracellular Wnt/β‐catenin signaling. Functionalized magnetic nanoparticles (MNPs@E/EC15) target cellular E‐cadherins to generate localized magnetomechanical forces under low‐intensity magnetic fields.
Christian Castro‐Hinojosa, Pablo Martínez‐Vicente, Lucía G. Recaredo, Susel Del Sol‐Fernández, Pilar Gomollón‐Zueco, Yilian Fernández‐Afonso, Raluca M. Fratila, María Moros   +7 more
wiley   +1 more source

High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2

Molecular Therapy: Methods & Clinical Development, 2019
Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies.
Naoya Uchida, Tina Nassehi, Claire M. Drysdale, Jackson Gamer, Morgan Yapundich, Selami Demirci, Juan J. Haro-Mora, Alexis Leonard, Matthew M. Hsieh, John F. Tisdale   +9 more
doaj   +1 more source

Chemical Screening Identifies Danofloxacin as a Self‐Renewal Agonist of Embryonic Stem Cells through Alleviation of HDAC1‐Mediated Deacetylation of Tert and Prdm10

Advanced Science, EarlyView.
This study identified Danofloxacin as a novel supporter of the maintenance of mouse embryonic stem cell (ESC) pluripotency. It functions partially by inhibiting HDAC1, which increases specific histone acetylation marks and activates key genes like Tert and Prdm10.
Yan Zhang, Junxiang Ji, Baoxing Dong, Zhenhua Zhu, Xucheng Lv, Peng Chen, Wenqiang Liu, Min Zhou, Shou‐Dong Ye   +8 more
wiley   +1 more source

Off-on polyadenylation strategy as a supplemental mechanism for silencing toxic transgene expression during lentiviral vector production

BioTechniques, 2014
Many gene therapy strategies rely on lentiviral-mediated transfer and expression of genes coding for toxic proteins. Methods of controlling transgene expression in target cells have been extensively investigated, but comparatively little attention has ...
Claude Bagnis, Gael Zwojsczyki, Jacques Chiaroni, Pascal Bailly   +3 more
doaj   +1 more source

Viral vector-mediated RNA interference in the retina [PDF]

, 2010
RNA interference (RNAi) is a highly conserved post-transcriptional gene silencing process triggered by double-stranded RNA (dsRNA) in eukaryotic cells.
Georgiadis, A.
core