Results 31 to 40 of about 411,689 (282)

Gene delivery to pancreatic exocrine cells in vivo and in vitro

BMC Biotechnology, 2012
Background Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested
Houbracken Isabelle, Baeyens Luc, Ravassard Philippe, Heimberg Harry, Bouwens Luc   +4 more
doaj   +1 more source

Prospects for transgenesis in the chick [PDF]

, 2004
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick
Sang, Helen
core   +1 more source

Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors

Journal of Biological Engineering, 2009
Background Lentiviral vectors with broad tropism are one of the most promising gene delivery systems capable of efficiently delivering genes of interest into both dividing and non-dividing cells while maintaining long-term transgene expression.
Wang Pin, Joo Kye-Il, Lei Yuning
doaj   +1 more source

Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 years

Journal of Biomedical Science, 2022
Viruses are naturally endowed with the capacity to transfer genetic material between cells. Following early skepticism, engineered viruses have been used to transfer genetic information into thousands of patients, and genetic therapies are currently ...
Jonas Holst Wolff, Jacob Giehm Mikkelsen
doaj   +1 more source

Designing lentiviral gene vectors [PDF]

, 2011
Lentiviral gene vectors are an important tool in gene therapy and basic biomedical research. They are transducing viral particles, normally replication defective, which are generated using the packaging machinery of lentiviruses.
Tanya Tolmachova, Faisal A., Al-Allaf, FA, Tolmachova, T, Tolmachov, OE, Oleg Tolmachov, Faisal A. Al-Allaf   +6 more
core   +1 more source

The stability of envelope-pseudotyped lentiviral vectors [PDF]

, 2020
Lentiviral vectors have become popular tools for stable genetic modification of mammalian cells. In some applications of lentiviral vector-transduced cells, infectious-lentiviral particles should be absent.
Rabelink, M.J.W.E., Dautzenberg, I.J.C., Hoeben, R.C.   +2 more
core   +1 more source

Hybrid Lentiviral Vectors

Molecular Therapy, 2010
Lentiviral vectors have remarkable cell entry and gene delivery properties that make them highly attractive for gene therapy. However, all integration-competent gene delivery systems have come under scrutiny for possible adverse insertional events. Circumventing the risk of insertional mutagenesis, integration-deficient human immunodeficiency virus ...
Qasim, Waseem, Vink, Conrad A, Thrasher, Adrian J   +2 more
openaire   +2 more sources

Effectiveness of gene delivery systems for pluripotent and differentiated cells

Molecular Therapy: Methods & Clinical Development, 2015
Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) assert a great future for the cardiovascular diseases, both to study them and to explore therapies. However, a comprehensive assessment of the viral vectors used to modify these
Kleopatra Rapti, Francesca Stillitano, Ioannis Karakikes, Mathieu Nonnenmacher, Thomas Weber, Jean-Sebastian Hulot, Roger J Hajjar   +6 more
doaj   +1 more source

Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types

mBio, 2017
We report a simple strategy for the creation of lentiviral vectors specific to any desired target cells. SpyTag is inserted into an engineered Sindbis virus envelope protein and displayed on the lentivirus surface to create Sindbis virus-SpyTag ...
Nagarjun Kasaraneni, Ana M. Chamoun-Emanuelli, Gus Wright, Zhilei Chen   +3 more
doaj   +1 more source

Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct

Molecular Therapy: Methods & Clinical Development, 2020
Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient ...
Yu Hua Chen, Celeste Pallant, Christopher J. Sampson, Alessia Boiti, Sabine Johnson, Pijus Brazauskas, Philip Hardwicke, Michela Marongiu, Vanesa M. Marinova, Marlene Carmo, Nathan P. Sweeney, Ashkenaz Richard, Anthony Shillings, Peter Archibald, Eva Puschmann, Bernadette Mouzon, David Grose, Miriam Mendez-Tavio, Mao Xiang Chen, Stephen R.C. Warr, Tarik Senussi, Paul S. Carter, Sean Baker, Cindy Jung, Martijn H. Brugman, Steven J. Howe, Conrad A. Vink   +26 more
doaj   +1 more source