Results 321 to 330 of about 94,541 (354)
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Purification of a lethal toxin produced by Shigella dysenteriae
Toxicon, 1982A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%.
Yoshifumi Takeda +2 more
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Anthrax lethal toxin: a weapon of multisystem destruction
Cellular and Molecular Life Sciences, 2004Lethal toxin (LT) is a major virulence factor secreted by anthrax bacteria. It is composed of two proteins, PA (protective antigen) and LF (lethal factor). PA transports the LF inside the cell, where LF, a zinc-dependent metalloprotease cleaves the mitogen activated protein kinase kinase (MAPKK) enzymes of the mitogen activated protein kinase (MAPK ...
A. Agrawal, B. Pulendran
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The Pathogenesis of the Lethal Effect of Anthrax Toxin in the Rat
Journal of Infectious Diseases, 1966ditions previously described (Beall et al, 1962). After 24 hours growth the bulk of the bacteria was removed by centrifugation, and the crude or unfractionated toxin used in these experiments was prepared in 1 of 2 ways: (a) the culture was sterilized by filtration through Millipore filters in stainless steel funnels, frozen, and stored at ?20 C; or (b)
Frederic G. Dalldorf, Francis A. Beall
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Molecular Microbiology, 1994
SummaryComparison of the anthrax toxin lethal factor (LF) amino acid sequence with sequences in the Swiss protein database revealed short regions of similarity with the consensus zinc‐binding site, HEXXH, that is characteristic of metalloproteases. Several protease inhibitors, including bestatin and captopril, prevented intoxication of macrophages by ...
Kurt Klimpel +2 more
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SummaryComparison of the anthrax toxin lethal factor (LF) amino acid sequence with sequences in the Swiss protein database revealed short regions of similarity with the consensus zinc‐binding site, HEXXH, that is characteristic of metalloproteases. Several protease inhibitors, including bestatin and captopril, prevented intoxication of macrophages by ...
Kurt Klimpel +2 more
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Protein and Peptide Letters, 2012
Lethal toxin of Clostridium sordellii (MLD 150 ng/kg) is one of the most potent Clostridial toxins and is responsible for most of the diseases including sudden death syndrome in cattle, sheep and toxic shock syndrome, necrotizing faciitis, neonatal omphalitis and gangrene in humans. Lethal toxin (TcsL) is a single chain protein of about 270 kDa. In the
G. B.K.S. Prasad +3 more
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Lethal toxin of Clostridium sordellii (MLD 150 ng/kg) is one of the most potent Clostridial toxins and is responsible for most of the diseases including sudden death syndrome in cattle, sheep and toxic shock syndrome, necrotizing faciitis, neonatal omphalitis and gangrene in humans. Lethal toxin (TcsL) is a single chain protein of about 270 kDa. In the
G. B.K.S. Prasad +3 more
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Novel Repression of the Glucocorticoid Receptor by Anthrax Lethal Toxin
Annals of the New York Academy of Sciences, 2004Abstract: Death from anthrax has been reported to occur from systemic shock. The lethal toxin (LeTx) is the major effector of anthrax mortality. Although the mechanism of entry of this toxin into cells is well understood, its actions once inside the cell are not as well understood. LeTx is known to cleave and inactivate MAPKKs.
Jeanette I. Webster +2 more
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Isolation of the major lethal toxin in the venom of Bungarus flaviceps
Toxicon, 2002The major lethal toxin in the venom of Bungarus flaviceps has been isolated by ion-exchange chromatography, absorption chromatography and RP-HPLC with a 14-fold purification and an overall yield of 16.5% of the lethal toxicity contained in crude venom. Its sublethal dose (LD(50)) determined in mice weighing 18-20 g was 0.25 (0.19-0.32) microg per mouse.
Orawan Khow +3 more
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Isolation of a lethal toxin from Cortinarius orellanus Fr
Mycopathologia, 1981The fungal toxin 'orellanine', isolated by Grzymala (10) from Cortinarius orellanus, seems to be a heterogeneous mixture of several toxic and nontoxic substances. We succeeded in isolating a lethal toxin which is sensitive to light and which is striking because of its long latency period. We consider this the main toxin of C. orellanus.
M. Moser, H Kürnsteiner
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Binding and cell intoxication studies of anthrax lethal toxin
Molecular Biology Reports, 2012Anthrax lethal toxin (LT) is a major virulence factor of Bacillus anthracis. The vast majority of the anthrax toxin-related literature describes the assembly of LT as a cell-dependent process. However, some reports have provided evidence for the existence of a fully assembled LT, either in vitro or in the bloodstream of anthrax-infected animals.
Steven W. Graves +2 more
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2017
Includes bibliographical references (leaves 50-53). ; In this study, we attempt to simultaneously target the major hallmarks of colorectal cancer (CRC), the MAPK pathway and the urokinase plasminogen activator system using a dual-selective, Urokinase-activated, recombinant anthrax lethal toxin (PrAgU2/LF).
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Includes bibliographical references (leaves 50-53). ; In this study, we attempt to simultaneously target the major hallmarks of colorectal cancer (CRC), the MAPK pathway and the urokinase plasminogen activator system using a dual-selective, Urokinase-activated, recombinant anthrax lethal toxin (PrAgU2/LF).
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