Results 121 to 130 of about 7,828,167 (206)
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Journal of Multidisciplinary HealthcareJian-Chun Hao,1,* Dan-Ni Dou,2,* Shao-Wei Cheng,1 Ruo-Yu Gou3,* 1Department of Dermatology, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, People’s Republic of China; 2School of Basic Medicine, Ningxia Medical ...
Hao JC, Dou DN, Cheng SW, Gou RY
doaj
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Association of Life's Crucial 9 with all-cause and cardiovascular mortality among the individuals with chronic inflammatory airway diseases: A prospective cohort study. [PDF]
Medicine (Baltimore)Wen Y +6 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
How Should We Respond to Life’s Senselessness?: Absurdity, Stoicism, and Playfulness
This project explores multiple responses to the question of life’s apparent ultimate senselessness, which is essentially the notion that there are not reasons, from the human perspective, for many of the events of our lives.
Neal, Mary
core +1 more source
The Inverse Association of Psoriasis and Life's Crucial 9 in US Adults: An Analysis from NHANES. [PDF]
J Multidiscip HealthcHao JC, Dou DN, Cheng SW, Gou RY.
europepmc +1 more source
Metaphysics of Life’s Worth: A Philosophical Inquiry
"Metaphysics of Life’s Worth: A Philosophical Inquiry" is a profound exploration of the essence of existence, meaning, and value. Written by Prof. Dr.
Prof. Dr. Yoesoep Edhie Rachmad, Ph.D, DBA
core