Results 61 to 70 of about 195,446 (304)

Reliability in the whole life cycle of building systems [PDF]

, 2006
Purpose – The purpose of this research is to show that reliability analysis and its implementation will lead to an improved whole life performance of the building systems, and hence their life cycle costs (LCC). Design/methodology/approach – This paper
Sidhu, J., Neale, K., Wu, Shaomin, Neale, Keith, Clements-Croome, Derek, Albany, B., Desmond, Duncan, Wu, S., Albany, Bob, Sidhu, Jogi, Fairey, Vic, Clements-Croome, Derek J., Desmond, D., Fairey, V.   +13 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

An AI-enhanced Approach for optimizing life cycle costing of military logistic vehicles

, 2022
296301Individual usage profile (aka true usage profile) of military logistic vehicles (MLV) is highly dependent on types of operations, duration and areas of use. Compared to non-military vehicles, the operation life is considerably longer, mileage tends
Ansari, Fazel, Sihn, Wilfried, Reichsthaler, Luisa, Madreiter, Theresa, Glawar, Robert, Giner, Jakob   +5 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Research on Highway Maintenance and Management Cost Based on Life Cycle Cost Analysis [PDF]

E3S Web of Conferences
With the rapid development of China’s highway construction, the huge demand for funds and the lack of financial strength of the contradiction is increasingly obvious, can effectively control the maintenance costs, highway operators to obtain sustainable ...
Li Jiarong, Gou Lijuan, Men Feng
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Decision support method for flat roofs with focus on life-cycle costs using a probabilistic method

, 2012
A probabilistic method based on a decision tree is summarized in this paper. The tool calculates the impact of a specific roof construction on the life cycle cost and energy efficiency of single family houses.
Bednar, Thomas, Nusser, Bernd, Harreither, Christoph   +2 more
core  

Life cycle cost comparison of design for disassembly and conventional prefab concrete buildings

Environmental Research: Infrastructure and Sustainability
This study explores the impact of design for disassembly (DfD) design solutions on the life cycle costs of a precast concrete building, comparing these costs with those of conventional design solutions.
Tommi Partanen, Ulrika Uotila, Tuomo Joensuu, Arto Saari   +3 more
doaj   +1 more source