Results 81 to 90 of about 1,949,985 (343)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Life cycle assessment and life cycle costs for pre-disaster waste management systems.

open access: yesWaste Management, 2017
This study develops a method of environmental and economic evaluation of an integrated disaster waste management system that considers the spatial scale of removal, transport, and treatment of disaster waste.
Y. Wakabayashi   +3 more
semanticscholar   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Solar array subsystems study [PDF]

open access: yes
The effects on life cycle costs of a number of technology areas are examined for a LEO, 500 kW solar array. A baseline system conceptual design is developed and the life cycle costs estimated in detail.
Badgley, M. B.   +2 more
core   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

Research on Highway Maintenance and Management Cost Based on Life Cycle Cost Analysis [PDF]

open access: yesE3S Web of Conferences
With the rapid development of China’s highway construction, the huge demand for funds and the lack of financial strength of the contradiction is increasingly obvious, can effectively control the maintenance costs, highway operators to obtain sustainable ...
Li Jiarong, Gou Lijuan, Men Feng
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Life cycle cost comparison of design for disassembly and conventional prefab concrete buildings

open access: yesEnvironmental Research: Infrastructure and Sustainability
This study explores the impact of design for disassembly (DfD) design solutions on the life cycle costs of a precast concrete building, comparing these costs with those of conventional design solutions.
Tommi Partanen   +3 more
doaj   +1 more source

Life cycle management of natural infrastructure: assessment of state of practice and current tools

open access: yesFrontiers in Built Environment
Design alternatives for traditional infrastructure are often compared in terms of expected–and often narrowly defined–costs and benefits to justify the selected plan.
Margaret H. Kurth   +4 more
doaj   +1 more source

Measuring the Welfare Costs of Inflation in a Life-cycle Model [PDF]

open access: yes
In macroeconomics, life-cycle models are typically used to address exclusively life-cycle issues. This paper shows that modeling the life-cycle may be important when addressing public policy issues, in this case the welfare costs of inflation.
Paul Gomme
core  

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