Prebiotic Factors Influencing the Activity of a Ligase Ribozyme [PDF]
Life, 2017 An RNA-lipid origin of life scenario provides a plausible route for compartmentalized replication of an informational polymer and subsequent division of the container.
Fabrizio Anella, Christophe Danelon
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The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life [PDF]
Biology, 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ...
Kana Tanizawa +4 more
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Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit [PDF]
Biology, 2023 The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga +3 more
doaj +6 more sources
Development of Allosteric Ribozymes for ATP and l-Histidine Based on the R3C Ligase Ribozyme [PDF]
LifeDuring the evolution of the RNA, short RNAs are thought to have joined together to form long RNAs, enhancing their function as ribozymes. Previously, the artificial R3C ligase ribozyme (73 nucleotides) was successfully reduced to 46 nucleotides; however,
Yuna Akatsu +2 more
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A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning [PDF]
Nature CommunicationsDelivering synthetic protein-coding RNA bypassing the DNA stage for ectopic protein functioning is a novel therapeutic strategy. Joining the linear RNA head-to-tail covalently could be a state-of-the-art strategy for functioning longer.
Chan-I Su +5 more
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Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability [PDF]
Life, 2014 The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge.
Fabrizio Anella, Christophe Danelon
doaj +8 more sources
RNA ligase ribozymes with a small catalytic core [PDF]
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
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Mapping L1 ligase ribozyme conformational switch. [PDF]
J Mol Biol, 2012 L1 ligase (L1L) molecular switch is an in vitro optimized synthetic allosteric ribozyme that catalyzes the regioselective formation of a 5'-to-3' phosphodiester bond, a reaction for which there is no known naturally occurring RNA catalyst. L1L serves as a proof of principle that RNA can catalyze a critical reaction for prebiotic RNA self-replication ...
Giambaşu GM, Lee TS, Scott WG, York DM.
europepmc +8 more sources
Development of a Functionally Minimized Mutant of the R3C Ligase Ribozyme Offers Insight into the Plausibility of the RNA World Hypothesis [PDF]
Biology, 2014 The R3C ligase ribozyme is an artificial ligase ribozyme produced by modification of the ribozyme that lacks cytidine. Here, we attempted to modify the original R3C ribozyme (73 nucleotides) by reducing the number of nucleotides while maintaining the ...
Eri Kurihara +3 more
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Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole to triphosphate activation. [PDF]
Proc Natl Acad Sci U S AABSTRACTThe acquisition of new RNA functions through evolutionary processes would have been essential for the diversification of RNA-based primordial biology and its subsequent transition to modern biology. However, the mechanisms by which RNAs access new functions remain unclear.
DasGupta S +3 more
europepmc +7 more sources