The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life [PDF]
Biology, 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ...
Kana Tanizawa +4 more
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Prebiotic Factors Influencing the Activity of a Ligase Ribozyme [PDF]
Life, 2017 An RNA-lipid origin of life scenario provides a plausible route for compartmentalized replication of an informational polymer and subsequent division of the container.
Fabrizio Anella, Christophe Danelon
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Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability [PDF]
Life, 2014 The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge.
Fabrizio Anella, Christophe Danelon
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Development of Allosteric Ribozymes for ATP and l-Histidine Based on the R3C Ligase Ribozyme [PDF]
LifeDuring the evolution of the RNA, short RNAs are thought to have joined together to form long RNAs, enhancing their function as ribozymes. Previously, the artificial R3C ligase ribozyme (73 nucleotides) was successfully reduced to 46 nucleotides; however,
Yuna Akatsu +2 more
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A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning [PDF]
Nature CommunicationsDelivering synthetic protein-coding RNA bypassing the DNA stage for ectopic protein functioning is a novel therapeutic strategy. Joining the linear RNA head-to-tail covalently could be a state-of-the-art strategy for functioning longer.
Chan-I Su +5 more
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Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit [PDF]
Biology, 2023 The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga +3 more
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RNA ligase ribozymes with a small catalytic core [PDF]
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
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Development of a Functionally Minimized Mutant of the R3C Ligase Ribozyme Offers Insight into the Plausibility of the RNA World Hypothesis [PDF]
Biology, 2014 The R3C ligase ribozyme is an artificial ligase ribozyme produced by modification of the ribozyme that lacks cytidine. Here, we attempted to modify the original R3C ribozyme (73 nucleotides) by reducing the number of nucleotides while maintaining the ...
Eri Kurihara +3 more
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Template-directed ligation of tethered mononucleotides by t4 DNA ligase for kinase ribozyme selection. [PDF]
PLoS ONE, 2010 BACKGROUND: In vitro selection of kinase ribozymes for small molecule metabolites, such as free nucleosides, will require partition systems that discriminate active from inactive RNA species.
David G Nickens +3 more
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Generation and Development of RNA Ligase Ribozymes with Modular Architecture Through “Design and Selection” [PDF]
Molecules, 2010 In vitro selection with long random RNA libraries has been used as a powerful method to generate novel functional RNAs, although it often requires laborious structural analysis of isolated RNA molecules.
Yuki Fujita +3 more
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