Results 1 to 10 of about 5,237 (166)
Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole to triphosphate activation. [PDF]
Proc Natl Acad Sci U S AThe acquisition of new RNA functions through evolutionary processes was essential for the diversification of RNA-based primordial biology and its subsequent transition to modern biology.
DasGupta S +3 more
europepmc +6 more sources
Prebiotic Factors Influencing the Activity of a Ligase Ribozyme. [PDF]
Life (Basel), 2017 An RNA-lipid origin of life scenario provides a plausible route for compartmentalized replication of an informational polymer and subsequent division of the container. However, a full narrative to form such RNA protocells implies that catalytic RNA molecules, called ribozymes, can operate in the presence of self-assembled vesicles composed of ...
Anella F, Danelon C.
europepmc +8 more sources
Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit. [PDF]
Biology (Basel), 2023 The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ~50 nucleotides, comparable to the estimated length of prebiotically synthesized RNA.
Kasuga M +3 more
europepmc +4 more sources
Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles. [PDF]
Nucleic Acids Res, 2019 Template-directed RNA ligation catalyzed by an RNA enzyme (ribozyme) is a plausible and important reaction that could have been involved in transferring genetic information during prebiotic evolution. Laboratory evolution experiments have yielded several
Nomura Y, Yokobayashi Y.
europepmc +3 more sources
Mapping L1 ligase ribozyme conformational switch. [PDF]
J Mol Biol, 2012 L1 ligase (L1L) molecular switch is an in vitro optimized synthetic allosteric ribozyme that catalyzes the regioselective formation of a 5'-to-3' phosphodiester bond, a reaction for which there is no known naturally occurring RNA catalyst. L1L serves as a proof of principle that RNA can catalyze a critical reaction for prebiotic RNA self-replication ...
Giambaşu GM, Lee TS, Scott WG, York DM.
europepmc +7 more sources
Reconciling ligase ribozyme activity with Fatty Acid vesicle stability. [PDF]
Life (Basel), 2014 The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge. One obstacle resides in the conflicting requirements for divalent metal ions, in particular Mg2+, with respect to optimal ribozyme activity, fatty acid vesicle ...
Anella F, Danelon C.
europepmc +8 more sources
The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life. [PDF]
Biology (Basel), 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ligation activity (decrease by two orders of magnitude compared to that of the ligated product of full ...
Tanizawa K +4 more
europepmc +6 more sources
Development of Allosteric Ribozymes for ATP and l-Histidine Based on the R3C Ligase Ribozyme. [PDF]
Life (Basel)During the evolution of the RNA, short RNAs are thought to have joined together to form long RNAs, enhancing their function as ribozymes. Previously, the artificial R3C ligase ribozyme (73 nucleotides) was successfully reduced to 46 nucleotides; however, its activity decreased significantly.
Akatsu Y, Mutsuro-Aoki H, Tamura K.
europepmc +4 more sources
Effects of lipid membranes on RNA catalytic activity and stability. [PDF]
Biol CellThis study explores how lipid membranes influence the activity and stability of natural ribozymes. We found that lipid membranes can both decrease and enhance ribozyme activity. We also observed that lipid membranes promote RNA degradation, depending on lipid composition and membrane fluidity.
Czerniak T, Saenz JP.
europepmc +2 more sources
Synthetic circRNA therapeutics: innovations, strategies, and future horizons. [PDF]
MedComm (2020)This pie chart visually represents the comprehensive content of this review, categorized into four main sections: circRNA synthesis, circRNA optimizations, delivery platforms, and circRNA therapeutics. Each section is further divided into specific subtopics, facilitating a quick and clear understanding of the key points covered in this review. circRNA,
Cai J +9 more
europepmc +2 more sources