Results 111 to 120 of about 73,558 (196)
Synthetic Biology, 2019 Abstract In vitro selection of ligand-responsive ribozymes can identify rare, functional sequences from large libraries. While powerful, key caveats of this approach include lengthy and demanding experimental workflows; unpredictable experimental outcomes and unknown functionality of enriched sequences in vivo.
Haines, M +4 more
openaire +6 more sources
Analytical Methods to Evaluate RNA Circularization Efficiency
ELECTROPHORESIS, Volume 45, Issue 21-22, Page 2005-2011, November 2024.ABSTRACT Circular RNAs (circRNAs) have emerged as pivotal players in RNA therapeutics. Unlike linear counterparts, circRNAs possess a closed‐loop structure, conferring them with enhanced stability and resistance to degradation. Ribozyme‐based strategy stands out as the predominant method for synthetic circRNA production, by precisely cleaving and ...
Yali Sun +3 more
wiley +1 more source
Nucleic acid enzymes for cleaving DNA [PDF]
, 2001 The present invention discloses nucleic acid enzymes capable of cleaving single-stranded DNA in a site specific ...
Joyce, Gerald F.
core +1 more source
Effective suppression of Dengue virus using a novel group-I intron that induces apoptotic cell death upon infection through conditional expression of the Bax C-terminal domain [PDF]
, 2014 Introduction: Approximately 100 million confirmed infections and 20,000 deaths are caused by Dengue virus (DENV) outbreaks annually. Global warming and rapid dispersal have resulted in DENV epidemics in formally non-endemic regions.
Carter, James R. +7 more
core +1 more source
Mini-ribozymes and freezing environment: a new scenario for the early RNA world
, 2005 International audienceThe RNA World hypothesis states that the present-day life, which is based on DNA genomes and protein enzymes, was preceded by a simpler life form based primarily on RNA.
Vlassov, A. V.
core +1 more source
Selection platforms for directed evolution in synthetic biology [PDF]
, 2016 Life on Earth is incredibly diverse. Yet, underneath that diversity, there are a number of constants and highly conserved processes: all life is based on DNA and RNA; the genetic code is universal; biology is limited to a small subset of potential ...
Amstutz +124 more
core +2 more sources
, 2009 Ribozimi su katalitičke molekule koje moraju biti savijene u visoko uređene tercijarne strukture, kao što to proteini rade da bi obavljali svoje funkcije. Glavni cilj je dovesti nukleofil i izrezujući fosfat u blizinu i aktivirati ih.
Zoko, Nikolina
core +2 more sources
Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection [PDF]
, 2015 published_or_final_versio
Cheung, YW +7 more
core +2 more sources
Escalation of error catastrophe for enzymatic self-replicators [PDF]
, 2007 It is a long-standing question in origin-of-life research whether the information content of replicating molecules can be maintained in the presence of replication errors.
B. Obermayer +5 more
core +2 more sources
The Structural Basis of Ribozyme-Catalyzed RNA Assembly [PDF]
, 2007 Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3 ...
Robertson, Michael P., Scott, William G.
core +1 more source