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In vitro adaptation of a ligase ribozyme for activity under a low‐pH condition
Biotechnology and Bioengineering, 2001AbstractA ligase ribozyme accelerating a ligation reaction with oligonucleotide under a low‐pH condition was selected by in vitro adaptation. A ribozyme active at pH 7 was randomly mutated, and the resultant RNA library was subjected to in vitro adaptation under a low‐pH reaction condition.
Yoshitaka Miyamoto +3 more
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A covalent crosslink converts the hammerhead ribozyme from a ribonuclease to an RNA ligase.
Nature structural biology, 2001The hammerhead ribozyme is a more efficient ribonuclease than an RNA ligase. Under typical reaction conditions, the rate of RNA chain cleavage is approximately 100-fold faster than the rate of the reverse ligation reaction such that virtually all of the hammerhead is in its cleaved form at equilibrium.
Olke C. Uhlenbeck +1 more
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Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme
Journal of Molecular Evolution, 2011RNA can function both as an informational molecule and as a catalyst in living organisms. This duality is the premise of the RNA world hypothesis. However, one flaw in the hypothesis that RNA was the most essential molecule in primitive life is that no RNA self-replicating system has been found in nature.
Tan Inoue +4 more
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A Simple Template-Dependent Ligase Ribozyme as the RNA Replicase Emerging First in the RNA World
Astrobiology, 2010The "RNA world" hypothesis has offered a framework for both experimental and theoretical work in the field of the origin of life. An important concern about the hypothesis is how the RNA world could originate. It has long been speculated that a template-dependent RNA synthetase ribozyme, which catalyzed its own replication (thus, an "RNA replicase ...
Wentao Zhang +3 more
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Biosystems, 2019
The formation of a kissing-loop through the introduction of complementary 7-membered loops is known to dramatically increase the activity of truncated R3C ligase ribozymes that otherwise display reduced activity. Restoration of activity is thought to result from kissing complex formation-induced rearrangement of two molecules with complementary loops ...
Kana Tanizawa +5 more
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The formation of a kissing-loop through the introduction of complementary 7-membered loops is known to dramatically increase the activity of truncated R3C ligase ribozymes that otherwise display reduced activity. Restoration of activity is thought to result from kissing complex formation-induced rearrangement of two molecules with complementary loops ...
Kana Tanizawa +5 more
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In vitro selection of novel ligase ribozymes [PDF]
Origins of life and evolution of the biosphere, 1996 Jack W. Szostak, Alicia J. Hager
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High-throughput screening method for promoter activity using bead display and a ligase ribozyme
Journal of Bioscience and Bioengineering, 2012In this report, we describe the development of a novel in vitro high-throughput system for detecting and screening promoter activity; the method employs emulsified reactions and a ligase ribozyme. In our study, a promoter DNA fragment containing the ribozyme gene was immobilized on a bead by using emulsion PCR, followed by in vitro transcription of the
Shoji Ohuchi +3 more
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E3 ligase ligand chemistries: from building blocks to protein degraders
Chemical Society Reviews, 2022Izidor Sosi +2 more
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E3 Ligase Ligands in Successful PROTACs: An Overview of Syntheses and Linker Attachment Points
Frontiers in Chemistry, 2021Aleša Bricelj +2 more
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