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Rapid discovery of functional RNA domains. [PDF]
Nucleic Acids ResLatifi B, Cole KH, Vu MMK, Lupták A.
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Long-read transcriptome-wide RNA structure maps using DMS-FIRST-seq
Begik O +5 more
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In vitro selection of a ligase ribozyme carrying alkylamino groups in the side chains.
Bioconjugate Chemistry, 2000A novel ligase ribozyme was in vitro selected from a random-sequence RNA library including N(6)-aminohexyl-modified adenine residues in place of natural adenine residues.
N. Teramoto, Y. Imanishi, Y. Ito
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Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme
Journal of Molecular Evolution, 2011RNA can function both as an informational molecule and as a catalyst in living organisms. This duality is the premise of the RNA world hypothesis. However, one flaw in the hypothesis that RNA was the most essential molecule in primitive life is that no RNA self-replicating system has been found in nature.
R. Ohmori +4 more
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In vitro adaptation of a ligase ribozyme for activity under a low-pH condition.
Biotechnology and Bioengineering, 2001A ligase ribozyme accelerating a ligation reaction with oligonucleotide under a low-pH condition was selected by in vitro adaptation. A ribozyme active at pH 7 was randomly mutated, and the resultant RNA library was subjected to in vitro adaptation under
Y. Miyamoto +3 more
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High-throughput screening method for promoter activity using bead display and a ligase ribozyme.
Journal of Bioscience and Bioengineering, 2012In this report, we describe the development of a novel in vitro high-throughput system for detecting and screening promoter activity; the method employs emulsified reactions and a ligase ribozyme. In our study, a promoter DNA fragment containing the ribozyme gene was immobilized on a bead by using emulsion PCR, followed by in vitro transcription of the
Takaaki Kojima +3 more
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In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups
Journal of Bioactive and Compatible Polymers, 2000Novel ribozymes containing 2'-amino groups in the side chains were in vitro selected to accelerate their ligation reaction rates with oligodeoxynucleotides. The ligation rate of random sequenced RNAs in the starting pool was accelerated by incorporation of 2'-amino-2'-deoxyuridine and N6-(6-aminohexyl)adenosine.
Yukio Imanishi +2 more
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Expanded divalent metal-ion tolerance of evolved ligase ribozymes
Biochimie, 2003Class I ligases are artificial ribozymes that catalyze the joining of two single-stranded RNAs. These ribozymes are between 120 and 160 nucleotides in length, making them intermediate in size for catalytic RNAs. Previous characterization of the b1-207 ribozyme suggests that it behaves similar to larger ribozymes in terms of divalent metal-ion ...
Craig A. Riley, Niles Lehman
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Artificial Ligase Ribozymes Isolated by a “Design and Selection” Strategy
2015We developed a new in vitro selection strategy "design and selection" to isolate effectively artificial ribozymes (catalytic RNAs). An overall RNA structure (scaffold) is initially designed, and then a relatively short randomized sequence is installed at the reaction point of the scaffold, followed by the in vitro selection.
Yoshiya Ikawa, Shigeyoshi Matsumura
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Biotechnology and Bioengineering, 2005
A ligase ribozyme that accelerates the ligation reaction with an oligonucleotide under low pH conditions was identified by in vitro adaptation in a previous study.
Y. Miyamoto +3 more
semanticscholar +1 more source
A ligase ribozyme that accelerates the ligation reaction with an oligonucleotide under low pH conditions was identified by in vitro adaptation in a previous study.
Y. Miyamoto +3 more
semanticscholar +1 more source