Nucleotide recognition and metal ion requirements of an RNA ligase ribozyme
, 2002 Margaret E. Glasner
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Direct testing of natural twister ribozymes from over a thousand organisms reveals a broad tolerance for structural imperfections. [PDF]
Nucleic Acids ResMcKinley LN +6 more
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A polymerase ribozyme that can synthesize both itself and its complementary strand
Gianni E +6 more
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Expanded divalent metal-ion tolerance of evolved ligase ribozymes
Biochimie, 2003Class I ligases are artificial ribozymes that catalyze the joining of two single-stranded RNAs. These ribozymes are between 120 and 160 nucleotides in length, making them intermediate in size for catalytic RNAs. Previous characterization of the b1-207 ribozyme suggests that it behaves similar to larger ribozymes in terms of divalent metal-ion ...
Craig A, Riley, Niles, Lehman
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Artificial Ligase Ribozymes Isolated by a “Design and Selection” Strategy
2015We developed a new in vitro selection strategy "design and selection" to isolate effectively artificial ribozymes (catalytic RNAs). An overall RNA structure (scaffold) is initially designed, and then a relatively short randomized sequence is installed at the reaction point of the scaffold, followed by the in vitro selection.
Shigeyoshi, Matsumura, Yoshiya, Ikawa
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Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme
Journal of Molecular Evolution, 2011RNA can function both as an informational molecule and as a catalyst in living organisms. This duality is the premise of the RNA world hypothesis. However, one flaw in the hypothesis that RNA was the most essential molecule in primitive life is that no RNA self-replicating system has been found in nature.
Rei, Ohmori +4 more
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The formation of a kissing-loop through the introduction of complementary 7-membered loops is known to dramatically increase the activity of truncated R3C ligase ribozymes that otherwise display reduced activity. Restoration of activity is thought to result from kissing complex formation-induced rearrangement of two molecules with complementary loops ...
Kokoro, Hamachi +5 more
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In vitro adaptation of a ligase ribozyme for activity under a low‐pH condition
Biotechnology and Bioengineering, 2001AbstractA ligase ribozyme accelerating a ligation reaction with oligonucleotide under a low‐pH condition was selected by in vitro adaptation. A ribozyme active at pH 7 was randomly mutated, and the resultant RNA library was subjected to in vitro adaptation under a low‐pH reaction condition.
Y, Miyamoto +3 more
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In Vitro Selection of a Ligase Ribozyme Carrying Alkylamino Groups in the Side Chains
Bioconjugate Chemistry, 2000A novel ligase ribozyme was in vitro selected from a random-sequence RNA library including N(6)-aminohexyl-modified adenine residues in place of natural adenine residues. This ribozyme mediated the formation of a phosphodiester bond with a DNA oligonucleotide through condensation with a 5'-triphosphate moiety on the ribozyme.
N, Teramoto, Y, Imanishi, Y, Ito
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High-throughput screening method for promoter activity using bead display and a ligase ribozyme
Journal of Bioscience and Bioengineering, 2012In this report, we describe the development of a novel in vitro high-throughput system for detecting and screening promoter activity; the method employs emulsified reactions and a ligase ribozyme. In our study, a promoter DNA fragment containing the ribozyme gene was immobilized on a bead by using emulsion PCR, followed by in vitro transcription of the
Takaaki, Kojima +3 more
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