Results 31 to 40 of about 5,237 (166)
Isolation of a ribozyme with 5′-5′ ligase activity
Chemistry & Biology, 1995 Many new ribozymes, including sequence-specific nucleases, ligases and kinases, have been isolated by in vitro selection from large pools of random-sequence RNAs. We are attempting to use in vitro selection to isolate new ribozymes that have, or can be evolved to have, RNA polymerase-like activities.
Karen B. Chapman, Jack W. Szostak
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Isolation of novel ribozymes that ligate AMP-activated RNA substrates [PDF]
, 1997 Background: The protein enzymes RNA ligase and DNA ligase catalyze the ligation of nucleic acids via an adenosine-5′-5′-pyrophosphate ‘capped’ RNA or DNA intermediate. The activation of nucleic acid substrates by adenosine 5′-monophosphate (AMP) may be a
Hager, Alicia J., Szostak, Jack W.
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Amplification of an RNA ligase ribozyme under alternating temperature conditions [PDF]
FEBS Letters, 2008 Amplification of an RNA template molecule was examined using the ligase ribozyme and its corresponding RNA substrates under alternating temperature conditions. Alternating temperatures enhanced the rate of the thermodynamically unfavorable dissociation of the annealed products into the two separate RNA templates, reminiscent of the polymerase chain ...
Moon-Young Yoon +4 more
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Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]
, 2020 Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P +3 more
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Metal Ion Requirements for Structure and Catalysis of an RNA Ligase Ribozyme [PDF]
Biochemistry, 2002 The class I ligase, a ribozyme previously isolated from random sequence, catalyzes a reaction similar to RNA polymerization, positioning its 5'-nucleotide via a Watson-Crick base pair, forming a 3',5'-phosphodiester bond between its 5'-nucleotide and the substrate, and releasing pyrophosphate.
David P. Bartel +2 more
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Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit
Angewandte Chemie, EarlyView.A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo +7 more
wiley +2 more sources
Large Phenotypic Enhancement of Structured Random RNA Pools. [PDF]
, 2020 Laboratory evolution of functional RNAs has applications in many areas of chemical and synthetic biology. In vitro selections critically depend on the presence of functional molecules, such as aptamers and ribozymes, in the starting sequence pools.
Chizzolini, Fabio +5 more
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The Structural Basis of Ribozyme-Catalyzed RNA Assembly [PDF]
, 2007 Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3 ...
Robertson, Michael P., Scott, William G.
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Assembly of a Ribozyme Ligase from Short Oligomers by Nonenzymatic Ligation
Journal of the American Chemical Society, 2020 Our current understanding of the chemistry of the primordial genetic material is fragmentary at best. The chemical replication of oligonucleotides long enough to perform catalytic functions is particularly problematic because of the low efficiency of nonenzymatic template copying.
Derek K. O'Flaherty +5 more
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Witnessing the Evolution of RNA [PDF]
, 2022 The RNA World theory postulates that at a certain point around 4 billion years ago during an RNA World period, a molecule, possibly RNA, developed the ability to self-replicate via polymerization.
Portillo, Xavier
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