Results 31 to 40 of about 5,652 (193)

Ribozyme activity modulates the physical properties of RNA–peptide coacervates

eLife, 2023
Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology.
Kristian Kyle Le Vay, Elia Salibi, Basusree Ghosh, TY Dora Tang, Hannes Mutschler   +4 more
doaj   +1 more source

Acquisition of Dual Ribozyme-Functions in Nonfunctional Short Hairpin RNAs through Kissing-Loop Interactions

Life, 2022
The acquisition of functions via the elongation of nucleotides is an important factor in the development of the RNA world. In our previous study, we found that the introduction of complementary seven-membered kissing loops into inactive R3C ligase ...
Hiromi Mutsuro-Aoki, Koji Tamura
doaj   +1 more source

Selection for Replicases in Protocells [PDF]

, 2013
PMCID: PMC3649988This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
A Kun, A Traulsen, A Wochner, AI Rushdi, AJ Dzieciol, AJ Markvoort, DE Kim, DW Deamer, E Szathmary, F Mavelli, G Yuen, Ginestra Bianconi, GU Yuen, GU Yuen, H Naraoka, HS Zaher, I Scheuring, IA Chen, IA Chen, Irene A. Chen, J Dworkin, JA Doudna, JF Fontanari, JG Lawless, JW Szostak, JW Szostak, K Kurihara, K Leu, KD James, Kun Zhao, M Eigen, M Eigen, M Leaver, M Nowak, M Wu, MA Nowak, MA Nowak, MA Nowak, Martin A. Nowak, Michael Doebeli, N Paul, N Takeuchi, N Vaidya, P Hogeweg, P Hogeweg, P Stano, P Szabo, P Walde, PG Higgs, RC Lewontin, RM Turk, SF Zenisek, SS Jain, SS Mansy, TA Lincoln, TF Zhu, TF Zhu, TM McCollom, UF Müller, V Vasas, WK Johnston, WV Allen   +61 more
core   +11 more sources

RNA-directed construction of structurally complex and active ligase ribozymes through recombination [PDF]

RNA, 2005
RNA-directed recombination can be used to catalyze a disproportionation reaction among small RNA substrates to create new combinations of sequences. But the accommodation of secondary and tertiary structural constraints in the substrates by recombinase ribozymes has not been explored.
Eric J. Hayden, Craig A. Riley, Aaron S. Burton, Niles Lehman   +3 more
openalex   +3 more sources

Partitioning the fitness components of RNA populations evolving in vitro. [PDF]

PLoS ONE, 2013
All individuals in an evolving population compete for resources, and their performance is measured by a fitness metric. The performance of the individuals is relative to their abilities and to the biotic surroundings--the conditions under which they are ...
Carolina Díaz Arenas, Niles Lehman
doaj   +1 more source

Effects of lipid membranes on RNA catalytic activity and stability. [PDF]

Biol Cell
This study explores how lipid membranes influence the activity and stability of natural ribozymes. We found that lipid membranes can both decrease and enhance ribozyme activity. We also observed that lipid membranes promote RNA degradation, depending on lipid composition and membrane fluidity.
Czerniak T, Saenz JP.
europepmc   +2 more sources

RNA folding and catalysis mediated by iron (II). [PDF]

PLoS ONE, 2012
Mg²⁺ shares a distinctive relationship with RNA, playing important and specific roles in the folding and function of essentially all large RNAs. Here we use theory and experiment to evaluate Fe²⁺ in the absence of free oxygen as a replacement for Mg²⁺ in
Shreyas S Athavale, Anton S Petrov, Chiaolong Hsiao, Derrick Watkins, Caitlin D Prickett, J Jared Gossett, Lively Lie, Jessica C Bowman, Eric O'Neill, Chad R Bernier, Nicholas V Hud, Roger M Wartell, Stephen C Harvey, Loren Dean Williams   +13 more
doaj   +1 more source

Isolation of a ribozyme with 5′-5′ ligase activity

Chemistry & Biology, 1995
Many new ribozymes, including sequence-specific nucleases, ligases and kinases, have been isolated by in vitro selection from large pools of random-sequence RNAs. We are attempting to use in vitro selection to isolate new ribozymes that have, or can be evolved to have, RNA polymerase-like activities.
Karen Chapman, Jack W. Szostak
openalex   +3 more sources

Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]

, 2020
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P, Ibrahim, AEC, Ma, J, Van Dolleweerd, CJ   +3 more
core   +1 more source

In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups

Journal of Bioactive and Compatible Polymers, 2000
Novel ribozymes containing 2'-amino groups in the side chains were in vitro selected to accelerate their ligation reaction rates with oligodeoxynucleotides. The ligation rate of random sequenced RNAs in the starting pool was accelerated by incorporation of 2'-amino-2'-deoxyuridine and N6-(6-aminohexyl)adenosine.
NAOZUMI TERAMOTO, YUKIO IMANISHI, Yoshihiro Ito   +2 more
openalex   +2 more sources