Results 41 to 50 of about 5,652 (193)

Force for ancient and recent life: viral and stem-loop RNA consortia promote life. [PDF]

, 2014
Lytic viruses were thought to kill the most numerous host (i.e., kill the winner). But persisting viruses/defectives can also protect against viruses, especially in a ubiquitous virosphere. In 1991, Yarmolinsky et al.
Villarreal, Luis P
core   +1 more source

A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination [PDF]

, 2010
RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic Fab library displayed on phage to obtain Fabs
A Desmyter, A Ke, A Serganov, AA Kossiakoff, AC Messias, AL Edwards, Anthony A Kossiakoff, AR Ferré-D'Amaré, AR Ferré-D'Amaré, AY Keel, BL Golden, BM Lunde, BS Saville, C Eigenbrot, C Höbartner, C Keryer-Bibens, CG Hoogstraten, D Piekna-Przybylska, David M Shechner, David P Bartel, DM Shechner, EA Kabat, EH Ekland, Erica M Duguid, FA Fellouse, FA Fellouse, FA Fellouse, IA Laird-Offringa, J-D Ye, JA Chao, JA Doudna, Jingdong Ye, Joseph A Piccirilli, JR Hogg, JR Hogg, JS Mattick, JS Mattick, K Decanniere, M Jinek, MJ Fedor, MP Robertson, N Carrasco, NH Bergman, Nikolai B Suslov, P Kapranov, PJ Carter, R Dutzler, RJ Taft, RK Montange, S Birtalan, S Iwata, S Jones, S Koide, S Koide, S Uysal, Sachdev S Sidhu, SC Bagby, Shohei Koide, SL Reichow, SR Eddy, SS Sidhu, TA Cooper, V Tereshko, Yelena Koldobskaya   +63 more
core   +2 more sources

Amplification of an RNA ligase ribozyme under alternating temperature conditions [PDF]

FEBS Letters, 2008
Amplification of an RNA template molecule was examined using the ligase ribozyme and its corresponding RNA substrates under alternating temperature conditions. Alternating temperatures enhanced the rate of the thermodynamically unfavorable dissociation of the annealed products into the two separate RNA templates, reminiscent of the polymerase chain ...
Kim, Ki-Sun, Oh, Sangtaek, Yea, Sung Su, Yoon, Moon-Young, Kim, Dong-Eun   +4 more
openaire   +2 more sources

Exploring the Potential and Advancements of Circular RNA Therapeutics [PDF]

Exploration (Beijing)
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Wang L, Wang L, Dong C, Liu J, Cui G, Gao S, Liu Z.   +6 more
europepmc   +2 more sources

Redesign of an Artificial Ligase Ribozyme Based on the Analysis of Its Structural Elements [PDF]

RNA Biology, 2005
The catalytic and folding properties of "DSL ribozyme" were investigated. This artificial ligase ribozyme was constructed by installing a catalytic unit to a designed self-folding RNA. The self-folding RNA was composed of three helices connected via two tertiary interactions that served as scaffolding in the molecular design.
Yoshiya Ikawa, Junko Matsumoto, Souta Horie, Tan Inoue   +3 more
openalex   +3 more sources

RNA catalysis in model protocell vesicles. [PDF]

, 2005
We are engaged in a long-term effort to synthesize chemical systems capable of Darwinian evolution, based on the encapsulation of self-replicating nucleic acids in self-replicating membrane vesicles.
Chen, Irene A, Salehi-Ashtiani, Kourosh, Szostak, Jack W   +2 more
core   +2 more sources

Kinetic Framework for Ligation by an Efficient RNA Ligase Ribozyme [PDF]

Biochemistry, 2000
The class I RNA ligase ribozyme, isolated previously from random sequences, performs an efficient RNA ligation reaction. It ligates two substrate RNAs, promoting the attack of the 3'-hydroxyl of one substrate upon the 5'-triphosphate of the other substrate with release of pyrophosphate.
N H, Bergman, W K, Johnston, D P, Bartel
openaire   +2 more sources

Riboswitch identification using Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR) [PDF]

Synthetic Biology, 2019
Abstract In vitro selection of ligand-responsive ribozymes can identify rare, functional sequences from large libraries. While powerful, key caveats of this approach include lengthy and demanding experimental workflows; unpredictable experimental outcomes and unknown functionality of enriched sequences in vivo.
Matthew Haines, Marko Storch, Diego A. Oyarzún, Guy‐Bart Stan, Geoff Baldwin   +4 more
openalex   +6 more sources

Mutational Analysis of Eggplant Latent Viroid RNA Circularization by the Eggplant tRNA Ligase in Escherichia coli

Frontiers in Microbiology, 2018
Eggplant latent viroid (ELVd) is a relatively small non-coding circular RNA that induces asymptomatic infections in eggplants (Solanum melongena L.). Like other viroid species that belong to the family Avsunviroidae, ELVd contains hammerhead ribozymes in
Teresa Cordero, Beltrán Ortolá, José-Antonio Daròs   +2 more
doaj   +1 more source

Beyond seek and destroy: How to generate allelic series using genome editing tools [PDF]

, 2020
Genome editing tools have greatly facilitated the functional analysis of genes of interest by targeted mutagenesis. Many usable genome editing tools, including different site-specific nucleases and editor databases that allow single-nucleotide ...
Bes, Martine, Chaine, Christian, Durandet, Franz, Guiderdoni, Emmanuel, Herbert, Leo, Meunier, Anne Cecile, Michel, Rémy, Portefaix, Murielle, Périn, Christophe, This, Patrice, Vernet, Aurore   +10 more
core   +3 more sources