Results 61 to 70 of about 5,652 (193)

Myth and fact in the origins of cellular life on Earth [PDF]

, 2017
Research into the origins of the first protocell is of a multidisciplinary nature. It draws evidence from what we know about the Earth’s early atmosphere and environment, and about the most ancient features of the cell’s structure and composition.
Jowett, P., Rayne, Richard, Tomas, Salvador   +2 more
core   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, Volume 599, Issue 24, Page 3569-3590, December 2025.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

RNA self-cleavage activated by ultraviolet light-induced oxidation [PDF]

, 2012
A novel UV-C-light-induced ribozyme activity was discovered within the highly structured 5′-genomic regions of both Hepatitis C Virus (HCV) and the related Classic Swine Fever Virus (CSFV).
Ariza-Mateos, Ascensión, Berzal-Herranz, Alfredo, Birk, A., Díaz-Toledano, Rosa, Gómez, J., Mena Piñeiro, Ignacio, Prieto-Vega, S., Szeto, H.   +7 more
core   +2 more sources

Optimizing (Bio) Catalysis with Liquid‐Liquid Phase Separation Systems

ChemSystemsChem, Volume 7, Issue 6, November 2025.
This review highlights how LLPS creates membraneless compartments involved in different scenarios in nature. It further explores different examples that focus on the development of synthetic coacervates that enhance catalytic efficiency by concentrating reactants, or by the direct influence of the coacervates building blocks themselves.
David Q. P. Reis, Joana Calvário, Inês Chibeles, Mohit Kumar, Ana S. Pina   +4 more
wiley   +1 more source

Protecting the proteome: Eukaryotic cotranslational quality control pathways. [PDF]

, 2014
The correct decoding of messenger RNAs (mRNAs) into proteins is an essential cellular task. The translational process is monitored by several quality control (QC) mechanisms that recognize defective translation complexes in which ribosomes are stalled on
Bennett, Eric J, Lykke-Andersen, Jens
core   +2 more sources

A stepwise emergence of evolution in the RNA world

FEBS Letters, Volume 599, Issue 19, Page 2706-2717, October 2025.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

A fast, efficient and sequence-independent method for flexible multiple segmental isotope labeling of RNA using ribozyme and RNase H cleavage [PDF]

, 2017
Structural information on RNA, emerging more and more as a major regulator in gene expression, dramatically lags behind compared with information on proteins.
Allain, Frédéric H.-T, Duss, Olivier, Maris, Christophe, von Schroetter, Christine   +3 more
core  

Probing complex RNA structures by mechanical force

, 2003
RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking
A. Xayaphoummine, Allemand, Cluzel, Cocco, D. Chatenay, Essevaz-Roulet, Evers, Fersht, Fontana, Gerland, Gutell, H. Isambert, Isambert, J-F. Léger, J. Robert, Kellermayer, Liphardt, Lubensky, Léger, Mathews, Montanari, Nowotny, Onoa, Powers, Rief, Rief, Rief, S. Harlepp, Smith, Svoboda, T. Marchal, Treiber, Treiber, Wimberly, Woodson, Xayaphoummine, Yusupov   +36 more
core   +3 more sources

Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol

Current Protocols, Volume 5, Issue 10, October 2025.
Abstract Allosteric transcription factors (aTFs) regulate gene expression in response to small molecules. Engineering aTFs with new ligand specificities expands their utility in environmental monitoring and diagnostics. Here, we present protocols for improving the responsiveness of an aTF, SRTF1, to its non‐native ligand, cortisol, based on the SELIS ...
Alia Clark‐ElSayed, Kaila E. Nayvelt, Satoshi Ishida, Simon d'Oelsnitz, Andrew D. Ellington   +4 more
wiley   +1 more source

The secondary structure and sequence optimization of an RNA ligase ribozyme

Nucleic Acids Research, 1995
In vitro selection can generate functional sequence variants of an RNA structural motif that are useful for comparative analysis. The technique is particularly valuable in cases where natural variation is unavailable or non-existent. We report the extension of this approach to a new extreme--the identification of a 112 nt ribozyme secondary structure ...
E H, Ekland, D P, Bartel
openaire   +3 more sources