Results 61 to 70 of about 5,237 (166)
ChemBioChem, Volume 26, Issue 10, May 27, 2025.Rational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Constanze Ebermann, Sabine Müller
wiley +1 more source
A fast, efficient and sequence-independent method for flexible multiple segmental isotope labeling of RNA using ribozyme and RNase H cleavage [PDF]
, 2017 Structural information on RNA, emerging more and more as a major regulator in gene expression, dramatically lags behind compared with information on proteins.
Allain, Frédéric H.-T +3 more
core
Isotope labeling strategies for NMR studies of RNA [PDF]
, 2009 The known biological functions of RNA have expanded in recent years and now include gene regulation, maintenance of sub-cellular structure, and catalysis, in addition to propagation of genetic information.
Lu, Kun +2 more
core +2 more sources
Structural Biology of RNA and Protein‐RNA Complexes after AlphaFold3
ChemBioChem, Volume 26, Issue 7, April 1, 2025.AlphaFold is a great research accelerator and hypothesis generator for structural biology and beyond. With the release of AlphaFold3, RNA and protein‐RNA complex structure prediction is readily possible. This review assesses the reliability of these predictions.
Janosch Hennig
wiley +1 more source
RNA-directed construction of structurally complex and active ligase ribozymes through recombination [PDF]
RNA, 2005 RNA-directed recombination can be used to catalyze a disproportionation reaction among small RNA substrates to create new combinations of sequences. But the accommodation of secondary and tertiary structural constraints in the substrates by recombinase ribozymes has not been explored.
Craig A. Riley +3 more
openaire +2 more sources
RNA-Catalyzed RNA Ligation on an External RNA Template [PDF]
Variants of the hc ligase ribozyme, which catalyzes ligation of the 3′ end of an RNA substrate to the 5′ end of the ribozyme, were utilized to evolve a ribozyme that catalyzes ligation reactions on an external RNA template. The evolved ribozyme catalyzes
Joyce, Gerald F, McGinness, Kathleen E
core +1 more source
Decoupling Activity and Specificity in Coronazymes
Small, Volume 21, Issue 14, April 9, 2025.A new Au nanoparticle (AuNP) based nanozyme, aptamer–coronazyme, which demonstrates ≈30 times higher activity on d‐glucose than the pristine AuNP nanozymes, is designed and characterized. This coronazyme shows long‐range chiral specificity controlled by circularly polarized lights and catalytic reactivity modulated by short‐range intermolecular forces.
Jiahao Ji +6 more
wiley +1 more source
Small, Volume 21, Issue 9, March 5, 2025.Microfluidic‐based directed evolution is a powerful tool for selection of biopolymers, however they struggle in transferability to intracellular environments. The “biomimetic equivalence” principle provides a versatile and practical framework for developing biopolymers that function effectively in cells using a cell‐free approach.
Andrew Brian Kinghorn +10 more
wiley +1 more source
The 2'-5' RNA Ligase of Escherichia coli: Purification, Cloning, and Genomic Disruption [PDF]
, 1996 An RNA ligase previously detected in extracts of Escherichia coli is capable of joining Saccharomyces cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2-5 phosphodiester linkage (Greer, C., Javor, B., and Abelson, J. (1983) Cell 33,
Abelson, John N., Arn, Eric A.
core
Physiologia Plantarum, Volume 177, Issue 2, March/April 2025.Abstract Viral infections are major contributors to crop yield loss and represent a significant threat to sustainable agriculture. Plants respond to virus attacks by activating sophisticated signalling cascades that initiate multiple defence mechanisms.
Kritika Shukla +4 more
wiley +1 more source