Results 61 to 70 of about 5,436 (190)

Investigation of the catalytic mechanism of RNase P: the role of divalent metal ions and functional groups important for catalysis [PDF]

, 2006
The ribonucleoprotein enzyme ribonuclease (RNase) P is an endonuclease that generates the mature 5' -ends of tRNA. Bacterial RNase P is composed of a large RNA subunit (P RNA) and a small protein (P protein). Studies with P RNA from E.
Cuzic-Feltens, Simona-Daniela, Hartmann, Roland K. (Prof. Dr.)   +1 more
core   +1 more source

Engineered Cas12j‐8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells

Advanced Science, EarlyView.
Engineered through structure‐guided protein engineering, enCas12j‐8 significantly enhances the editing efficiency of compact Cas12j‐8 while maintaining high specificity. It enables efficient multiplexed genome editing and base editing using a single crRNA array, demonstrating broad applicability and therapeutic potential in genome engineering ...
Ru Meng, Jiayao Li, Wuke Wang, Dong Liang, Zhanwei Li, Cong Mao, Qingyang Li, Yu Zhang, Hao Chen, Jin Tang, Ping Hu, Qi Niu, Xingxu Huang, Bin Shen, Jun Zhang   +14 more
wiley   +1 more source

In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production [PDF]

, 2011
According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity,
Ayaz Ali Khan, Israr Khan, Muhammad Waheed Akktar   +2 more
core   +1 more source

Continuous In Vitro evolution of a ribozyme ligase: A model experiment for the evolution of a biomolecule [PDF]

Biochemistry and Molecular Biology Education, 2013
AbstractEvolution is a defining criterion of life and is central to understanding biological systems. However, the timescale of evolutionary shifts in phenotype limits most classroom evolution experiments to simple probability simulations. In vitro directed evolution (IVDE) frequently serves as a model system for the study of Darwinian evolution but ...
Michael P. Ledbetter, Andrew D. Ellington, Gwendolyn M. Stovall, Tony W. Hwang   +3 more
openaire   +3 more sources

Exploring the Potential and Advancements of Circular RNA Therapeutics

Exploration, EarlyView.
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang, Lianqing Wang, Chunbo Dong, Jialong Liu, Guoxin Cui, Shan Gao, Zhida Liu   +6 more
wiley   +1 more source

Selective Nonenzymatic Formation of Biologically Common RNA Hairpins

Angewandte Chemie, Volume 137, Issue 5, January 27, 2025.
Template‐free RNA loop‐closing ligation selectively generates RNA tetraloops that are prevalent in biology, as demonstrated by high‐throughput sequencing. Utilizing high‐yield overhang sequences enables the efficient assembly of functional RNAs. Abstract The prebiotic formation of RNA building blocks is well‐supported experimentally, yet the emergence ...
Long‐Fei Wu, Junting Zhang, Robert Cornwell‐Arquitt, David A. Hendrix, Aleksandar Radakovic, Jack W. Szostak   +5 more
wiley   +2 more sources

Synthetic shuffling and in vitro selection reveal the rugged adaptive fitness landscape of a kinase ribozyme [PDF]

, 2012
The relationship between genotype and phenotype is often described as an adaptive fitness landscape. In this study, we used a combination of recombination, in vitro selection, and comparative sequence analysis to characterize the fitness landscape of a ...
Bartel, David, Curtis, Edward A.
core   +1 more source

Advances in Isotope Labeling for Solution Nucleic Acid Nuclear Magnetic Resonance Spectroscopy

ChemPlusChem, Volume 90, Issue 7, July 2025.
Recent advances in the field of nucleic acid nuclear magnetic resonance (NMR) are presented. Stable isotope labeling protocols and new approaches to segmentally label nucleic acid with NMR‐active residues are summarized. The novel methods will have a strong impact on the future of nucleic acid NMR and the characterization of structure and dynamics of ...
Stefan Hilber, Solomon Kojo Attionu, Theodore Kwaku Dayie, Christoph Kreutz   +3 more
wiley   +1 more source

Probing complex RNA structures by mechanical force

, 2003
RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking
A. Xayaphoummine, Allemand, Cluzel, Cocco, D. Chatenay, Essevaz-Roulet, Evers, Fersht, Fontana, Gerland, Gutell, H. Isambert, Isambert, J-F. Léger, J. Robert, Kellermayer, Liphardt, Lubensky, Léger, Mathews, Montanari, Nowotny, Onoa, Powers, Rief, Rief, Rief, S. Harlepp, Smith, Svoboda, T. Marchal, Treiber, Treiber, Wimberly, Woodson, Xayaphoummine, Yusupov   +36 more
core   +3 more sources

Genomic and biochemical comparison of allelic triple‐mutant lines derived from conventional breeding and multiplex gene editing

The Plant Genome, Volume 18, Issue 2, June 2025.
Abstract Multiplex gene editing allows for the simultaneous targeting and mutagenesis of multiple loci in a genome. This tool is particularly valuable for plant genetic improvement, as plant genomes often require mutations at multiple loci to confer useful and/or novel traits.
Junqi Liu, Ritesh Kumar, Samatha Gunapati, Steven Mulkey, Yinjie Qiu, Yer Xiong, Vishnu Ramasubramanian, Jean‐Michel Michno, Praveen Awasthi, Daniel D. Gallaher, Thi Thao Nguyen, Won‐Seok Kim, Hari B. Krishnan, Aaron J. Lorenz, Robert M. Stupar   +14 more
wiley   +1 more source