Results 71 to 80 of about 5,652 (193)
Investigation of the catalytic mechanism of RNase P: the role of divalent metal ions and functional groups important for catalysis [PDF]
, 2006 The ribonucleoprotein enzyme ribonuclease (RNase) P is an endonuclease that generates the mature 5' -ends of tRNA. Bacterial RNase P is composed of a large RNA subunit (P RNA) and a small protein (P protein). Studies with P RNA from E.
Cuzic-Feltens, Simona-Daniela +1 more
core +1 more source
Engineered Cas12j‐8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells
Advanced Science, Volume 12, Issue 33, September 4, 2025.Engineered through structure‐guided protein engineering, enCas12j‐8 significantly enhances the editing efficiency of compact Cas12j‐8 while maintaining high specificity. It enables efficient multiplexed genome editing and base editing using a single crRNA array, demonstrating broad applicability and therapeutic potential in genome engineering ...
Ru Meng +14 more
wiley +1 more source
The dynamics of the RNA world : Insights and challenges [PDF]
, 2015 The problem of the origin of life is not only one of structure but also that of dynamics. Ever since the seminal result of Manfred Eigen in 1971 showing that early template replication suffers from an error threshold, research has tackled the issue of ...
Boza, Gergely +5 more
core +4 more sources
Quasispecies evolution in general mean-field landscapes [PDF]
, 2001 I consider a class of fitness landscapes, in which the fitness is a function of a finite number of phenotypic "traits", which are themselves linear functions of the genotype.
Baake E. +9 more
core +1 more source
, 2021 Numerous living systems are hierarchically organised, whereby replicating components are grouped into reproducing collectives -- e.g., organelles are grouped into cells, and cells are grouped into multicellular organisms.
Kaneko, Kunihiko +2 more
core +1 more source
Mitochondrial Diseases: Molecular Pathogenesis and Therapeutic Advances
MedComm, Volume 6, Issue 9, September 2025.Key pathogenic mechanisms—OXPHOS defects, heteroplasmy, impaired mtDNA repair, disrupted dynamics/mitophagy, and inflammatory signaling—drive multiorgan involvement in mitochondrial diseases. Targeted interventions include metabolic drugs (CoQ10, idebenone, EPI‐743), mitochondrial gene editing (DdCBE, mitoBEs, CyDENT/mitoTALEN/mtZFN), and mitochondrial
Jialun Mei +6 more
wiley +1 more source
Searching for a DNAzyme version of the leadzyme [PDF]
, 2015 The final publication is available at Springer via http://dx.doi.org/10.1007/s00239-015-9702-zThe leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired
Chen, Qingyun +2 more
core +1 more source
, 2004 Nucleozymes containing ribonucleotides and deoxyribonucleotides or nucleic acid analogues are described herein. The nucleozymes have catalytic activity and are significantly more resistant to degradation than their all-RNA ribozyme counterparts.
Cedergren, Robert J. +4 more
core +1 more source
Incorporation of aptamers in the terminal loop of shRNAs yields an effective and novel combinatorial targeting strategy. [PDF]
, 2017 Gene therapy by engineering patient's own blood cells to confer HIV resistance can potentially lead to a functional cure for AIDS. Toward this goal, we have previously developed an anti-HIV lentivirus vector that deploys a combination of shRNA, ribozyme ...
Castanotto, Daniela +4 more
core +1 more source
Advances in Isotope Labeling for Solution Nucleic Acid Nuclear Magnetic Resonance Spectroscopy
ChemPlusChem, Volume 90, Issue 7, July 2025.Recent advances in the field of nucleic acid nuclear magnetic resonance (NMR) are presented. Stable isotope labeling protocols and new approaches to segmentally label nucleic acid with NMR‐active residues are summarized. The novel methods will have a strong impact on the future of nucleic acid NMR and the characterization of structure and dynamics of ...
Stefan Hilber +3 more
wiley +1 more source