Results 71 to 80 of about 5,237 (166)

Selection for Replicases in Protocells [PDF]

, 2013
PMCID: PMC3649988This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
A Kun, A Traulsen, A Wochner, AI Rushdi, AJ Dzieciol, AJ Markvoort, DE Kim, DW Deamer, E Szathmary, F Mavelli, G Yuen, Ginestra Bianconi, GU Yuen, GU Yuen, H Naraoka, HS Zaher, I Scheuring, IA Chen, IA Chen, Irene A. Chen, J Dworkin, JA Doudna, JF Fontanari, JG Lawless, JW Szostak, JW Szostak, K Kurihara, K Leu, KD James, Kun Zhao, M Eigen, M Eigen, M Leaver, M Nowak, M Wu, MA Nowak, MA Nowak, MA Nowak, Martin A. Nowak, Michael Doebeli, N Paul, N Takeuchi, N Vaidya, P Hogeweg, P Hogeweg, P Stano, P Szabo, P Walde, PG Higgs, RC Lewontin, RM Turk, SF Zenisek, SS Jain, SS Mansy, TA Lincoln, TF Zhu, TF Zhu, TM McCollom, UF Müller, V Vasas, WK Johnston, WV Allen   +61 more
core   +5 more sources

A general method for phasing novel complex RNA crystal structures without heavy-atom derivatives [PDF]

, 2008
Using idealized known RNA secondary-structural fragments, it is demonstrated that it is possible to solve novel complex RNA structures without resort to heavy-atom phasing ...
Robertson, Michael P., Scott, William G.
core   +2 more sources

Selection for Catalytic Function with Nucleic Acids [PDF]

, 2000
For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself.
Frank D.N., Gilbert W., Joyce G.F., Sambrook J.   +3 more
core   +1 more source

Aptasensors and Advancement in Molecular Recognition Technology

Advanced Materials Technologies, Volume 10, Issue 1, January 8, 2025.
This review summarizes how aptamers (chemical antibodies)‐based biosensors are developed and applied to biological and biomedical research as well as clinical diagnostics. How a nucleic acid‐based biosensor platform is poised to become the next generation of sensitive and cost‐effective technology will be showcased, shaping the future of biosensing ...
Rajindra Napit, Satendra Kumar Jaysawal, Rocky Chowdhury, Jasmine Catague, Haben Melke, Cuong Viet Pham, Huo Xu, Lee Jia, Jia Lin, Yingchu Hou, Wei Duan   +10 more
wiley   +1 more source

Elucidating Evolutionary Mechanisms and Variants of the Hammerhead Ribozyme Using In Vitro Selection

ChemBioChem, Volume 25, Issue 21, November 4, 2024.
We generated single‐, double‐, and triple‐mutation libraries to explore the sequence requirements and evolution of a full‐length hammerhead ribozyme. We identified many hammerhead variants, some of which possess mutations at previously considered essential nucleotides. We also present a novel methodological approach for evaluating the sequence‐function
Jake Brill, Connor Nurmi, Yingfu Li
wiley   +1 more source

Analytical Methods to Evaluate RNA Circularization Efficiency

ELECTROPHORESIS, Volume 45, Issue 21-22, Page 2005-2011, November 2024.
ABSTRACT Circular RNAs (circRNAs) have emerged as pivotal players in RNA therapeutics. Unlike linear counterparts, circRNAs possess a closed‐loop structure, conferring them with enhanced stability and resistance to degradation. Ribozyme‐based strategy stands out as the predominant method for synthetic circRNA production, by precisely cleaving and ...
Yali Sun, Anis H. Khimani, Yanhong Tong, Zhi‐xiang Lu   +3 more
wiley   +1 more source

Site-specific isotope labeling of long RNA for structural and mechanistic studies [PDF]

, 2010
A site-specific isotope labeling technique of long RNA molecules was established. This technique is comprised of two simple enzymatic reactions, namely a guanosine transfer reaction of group I self-splicing introns and a ligation with T4 DNA ligase.
Barfield, Bass, Batey, Batey, Been, Been, Burke, Burke, Cech, Cech, Chojiro Kojima, Daichi Yamanaka, Davies, Delaglio, Dingley, Doudna, Fan, Hiroyuki Furuta, Ikawa, Ikawa, Ikumi Kawahara, Ishikawa, Junya Ishikawa, Kaichiro Haruta, Kao, Kenta Teruya, Kim, Kim, Kojima, Kruger, Lippard, Lu, Martick, Michel, Michnicka, Mituhiro Kuriyama, Murphy, Naoko Toki, Nikonowicz, Nikonowicz, Ohtsuki, Pervushin, Pley, Polson, Scott, Shan, Strobel, Szostak, Tanaka, Tanaka, Tanaka, Tanaka, Tanaka, Tanaka, Tzakos, Vogt, Wang, Williams, Williamson, Wu, Xu, Yoshinori Kondo, Yoshiya Ikawa, Yoshiyuki Tanaka, Young, Yuta Ashihara, Zaug, Zhang   +67 more
core   +2 more sources

Empirical Investigations OF RNA Fitness Landscapes: Harnessing the Power of High-Throughput Sequencing and Evolutionary Simulations [PDF]

, 2018
Fitness landscapes or adaptive landscapes represent the mapping of genotype (sequence) to phenotype (function or fitness). Originally proposed as a metaphor to envision evolutionary processes and mutational interactions, the fitness landscape has ...
Bendixsen, Devin Pratt
core   +1 more source

New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference

Molecular Plant Pathology, Volume 25, Issue 10, October 2024.
Rice tiller inhibition virus 2, originating from wild rice, carries mutations at residues that are broadly conserved among poleroviruses, and these mutations weaken viral suppression of RNAi, allowing for persistent infection. Abstract Persistent plant viruses are widespread in natural ecosystems. However, little is known about why persistent infection
Li‐Juan Zhu, Yu Zhu, Chengwu Zou, Lan‐Yi Su, Chong‐Tao Zhang, Chi Wang, Ya‐Ni Bai, Baoshan Chen, Rongbai Li, Qingfa Wu, Shou‐Wei Ding, Jian‐Guo Wu, Yan‐Hong Han   +12 more
wiley   +1 more source

Expanding the Scope of Ribosome‐Mediated Biosynthesis in vitro using tRNA‐Aminoacylating Ribozyme

Israel Journal of Chemistry, Volume 64, Issue 8-9, September 2024.
Abstract Proteins are synthesized within ribosomes through the polymerization of amino acids (AAs). This process requires prior activation of AAs through aminoacylation that attaches them to their corresponding transfer RNAs (tRNAs). Within cells, this attachment is facilitated by aminoacyl‐tRNA synthetase, resulting in a tRNA:AA conjugate.
Namjin Cho, Haneul Jin, Hyewon Jeon, Kanghun Lee, Joongoo Lee   +4 more
wiley   +1 more source