Results 81 to 90 of about 5,436 (190)
The first peptides: the evolutionary transition between prebiotic amino acids and early proteins [PDF]
The issues we attempt to tackle here are what the first peptides did look like when they emerged on the primitive earth, and what simple catalytic activities they fulfilled.
Buhrman, Harry+4 more
core +5 more sources
Nucleozymes containing ribonucleotides and deoxyribonucleotides or nucleic acid analogues are described herein. The nucleozymes have catalytic activity and are significantly more resistant to degradation than their all-RNA ribozyme counterparts.
Cedergren, Robert J.+4 more
core +1 more source
Myth and fact in the origins of cellular life on Earth [PDF]
Research into the origins of the first protocell is of a multidisciplinary nature. It draws evidence from what we know about the Earth’s early atmosphere and environment, and about the most ancient features of the cell’s structure and composition.
Jowett, P.+2 more
core +1 more source
Aptasensors and Advancement in Molecular Recognition Technology
This review summarizes how aptamers (chemical antibodies)‐based biosensors are developed and applied to biological and biomedical research as well as clinical diagnostics. How a nucleic acid‐based biosensor platform is poised to become the next generation of sensitive and cost‐effective technology will be showcased, shaping the future of biosensing ...
Rajindra Napit+10 more
wiley +1 more source
Searching for a DNAzyme version of the leadzyme [PDF]
The final publication is available at Springer via http://dx.doi.org/10.1007/s00239-015-9702-zThe leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired
Chen, Qingyun+2 more
core +1 more source
Elucidating Evolutionary Mechanisms and Variants of the Hammerhead Ribozyme Using In Vitro Selection
We generated single‐, double‐, and triple‐mutation libraries to explore the sequence requirements and evolution of a full‐length hammerhead ribozyme. We identified many hammerhead variants, some of which possess mutations at previously considered essential nucleotides. We also present a novel methodological approach for evaluating the sequence‐function
Jake Brill, Connor Nurmi, Yingfu Li
wiley +1 more source
Design and synthesis of probes for detection of protein-protein interaction and RNA localization [PDF]
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.Includes bibliographical references.The use of the ketone biotin - benzophenone-biotin hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB ...
Ryan, Jeremy Adam
core
Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates [PDF]
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biochemistry, copyright © American Chemical Society after peer review and technical editing by publisher.
Huang, Po-Jung Jimmy+3 more
core +1 more source
Analytical Methods to Evaluate RNA Circularization Efficiency
ABSTRACT Circular RNAs (circRNAs) have emerged as pivotal players in RNA therapeutics. Unlike linear counterparts, circRNAs possess a closed‐loop structure, conferring them with enhanced stability and resistance to degradation. Ribozyme‐based strategy stands out as the predominant method for synthetic circRNA production, by precisely cleaving and ...
Yali Sun+3 more
wiley +1 more source
Abstract In vitro selection of ligand-responsive ribozymes can identify rare, functional sequences from large libraries. While powerful, key caveats of this approach include lengthy and demanding experimental workflows; unpredictable experimental outcomes and unknown functionality of enriched sequences in vivo.
Haines, M+4 more
openaire +6 more sources