Results 81 to 90 of about 5,237 (166)

Strategies for Detecting Aminoacylation and Aminoacyl‐tRNA Editing in vitro and in Cells

Israel Journal of Chemistry, Volume 64, Issue 8-9, September 2024.
Abstract Aminoacyl‐tRNA synthetases (aaRSs) maintain translational fidelity by ensuring the formation of correct aminoacyl‐tRNA pairs. Numerous point mutations in human aaRSs have been linked to disease phenotypes. Structural studies of aaRSs from human pathogens encoding unique domains support these enzymes as potential candidates for therapeutics ...
Rylan R. Watkins, Arundhati Kavoor, Karin Musier‐Forsyth   +2 more
wiley   +1 more source

Evolutionary Dynamics in Molecular Populations of Ligase Ribozymes

, 2000
The emergence of life depended on the ability of the first biopolymer populations to thrive and approach larger population sizes and longer sequences that could store enough information, as required for a cellular type of life. The evolution of these populations very likely occurred under circumstances under which Muller’s Ratchet in synergism with ...
openaire   +3 more sources

Coordinated control of a designed trans‐acting ligase ribozyme by a loop–receptor interaction

FEBS Letters, 2009
We previously developed a synthetic cis‐acting RNA ligase ribozyme with 3′–5′ joining activity termed “DSL” (designed and selected ligase). DSL was easily transformed into a trans‐acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans‐acting DSL, tDSL‐1/GUAA ...
Shigeyoshi Matsumura, Rei Ohmori, Hirohide Saito, Tan Inoue, Yoshiya Ikawa, Yoshiya Ikawa   +5 more
openaire   +3 more sources

INSIGHTS INTO ENZYMATIC MANIPULATIONS OF NUCLEIC ACIDS [PDF]

, 2005
This dissertation details three studies dealing with the manipulation of nucleicacids. In the first investigation, each of the four natural nucleobases were analyzed for theability to serve as a universal template at the ligation junction of a T4 DNA ...
Alexander, Rashada Corine
core   +1 more source

Deoxyribozymes that recode sequence information [PDF]

, 2006
Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected.
Ellington, Andrew D., Levy, Matthew, Tabor, Jeffrey J.   +2 more
core   +3 more sources

Probing complex RNA structures by mechanical force

, 2003
RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking
A. Xayaphoummine, Allemand, Cluzel, Cocco, D. Chatenay, Essevaz-Roulet, Evers, Fersht, Fontana, Gerland, Gutell, H. Isambert, Isambert, J-F. Léger, J. Robert, Kellermayer, Liphardt, Lubensky, Léger, Mathews, Montanari, Nowotny, Onoa, Powers, Rief, Rief, Rief, S. Harlepp, Smith, Svoboda, T. Marchal, Treiber, Treiber, Wimberly, Woodson, Xayaphoummine, Yusupov   +36 more
core   +3 more sources

Viroid Replication: Rolling-Circles, Enzymes and Ribozymes [PDF]

, 2009
Viroids, due to their small size and lack of protein-coding capacity, must rely essentially on their hosts for replication. Intriguingly, viroids have evolved the ability to replicate in two cellular organella, the nucleus (family Pospiviroidae) and the ...
Alberto Carbonell, Ambrós, Ambrós, Astier-Manifacier, Branch, Branch, Chi, Diego Molina-Serrano, Flores, Flores, José-Antonio Daròs, Kibertis, Marcos De la Peña, María-Eugenia Gas, María-Ángeles Nohales, Ricardo Flores, Schiebel, Selma Gago, Sharmeen, Symons, Tabler   +20 more
core   +4 more sources

Key Labeling Technologies to Tackle Sizeable Problems in RNA Structural Biology [PDF]

, 2008
The ability to adopt complex three-dimensional (3D) structures that can rapidly interconvert between multiple functional states (folding and dynamics) is vital for the proper functioning of RNAs.
Amaral, Anderson, Azarani, Bain, Batey, Batey, Batey, Baugh, Bessonov, Boisvert, Brandt, Brutscher, Cate, Cheong, Coleman, Correll, Cromsigt, Crowe, D'Souza, D'Souza, D'Souza, Davis, Dayie, Dayie, Dayie, Dayie, Dickman, Dieckmann, Dolinnaya, Edwards, Eldho, Ferre-D'Amare, Ferre-D'Amare, Ferré-D'Amaré, Furtig, Gardner, Golden, Golden, Golden, Golden, Grosshans, Gumbs, Hampel, Helm, Hennig, Hines, Hirschbein, Hoffman, Hoogstraten, Höbartner, Johnson, Kao, Ke, Keel, Kieft, Kim, Kim, Korostelev, Krieg, Krupp, Kwaku Dayie, Latham, Latham, LeMaster, Lukavsky, Markley, Markus, Martick, McIntosh, McKenna, Michnicka, Milligan, Mitra, Moore, Moran, Nikonowicz, Nikonowicz, Pardi, Peterson, Pleiss, Pokrovskaya, Ponchon, Puglisi, Purtha, Pyle, Rising, Romaniuk, Scaringe, Scott, Scott, Seetharaman, Sheng, Shields, Sich, Simon, Sokolova, Stark, Stark, Steitz, Studier, Szymczyna, Taft, Tolbert, Tolbert, Toor, Toth, Tzakos, Tzakos, Vallurupalli, Wakeman, Wedekind, Wijmenga, Wincott, Winkler, Wyatt, Ying   +115 more
core   +4 more sources

Mini-ribozymes and freezing environment: a new scenario for the early RNA world

, 2005
International audienceThe RNA World hypothesis states that the present-day life, which is based on DNA genomes and protein enzymes, was preceded by a simpler life form based primarily on RNA.
Vlassov, A. V.
core   +1 more source

The molecular evolution of complexity at the origin of life [PDF]

, 2016
A thesis submitted to the Faculty of Health Sciences, University of the Witwatersrand, in fulfilment of the requirements for the degree of Doctor of Philosophy Johannesburg, 2016A widely accepted hypothesis for the origin of life is that it was based ...
Dhar, Nisha
core