Results 81 to 90 of about 5,652 (193)
The Plant Genome, Volume 18, Issue 2, June 2025.Abstract Multiplex gene editing allows for the simultaneous targeting and mutagenesis of multiple loci in a genome. This tool is particularly valuable for plant genetic improvement, as plant genomes often require mutations at multiple loci to confer useful and/or novel traits.
Junqi Liu +14 more
wiley +1 more source
The 2'-5' RNA Ligase of Escherichia coli: Purification, Cloning, and Genomic Disruption [PDF]
, 1996 An RNA ligase previously detected in extracts of Escherichia coli is capable of joining Saccharomyces cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2-5 phosphodiester linkage (Greer, C., Javor, B., and Abelson, J. (1983) Cell 33,
Abelson, John N., Arn, Eric A.
core
A minimal biochemical route towards de novo formation of synthetic phospholipid membranes. [PDF]
, 2019 All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function.
Bhattacharya, Ahanjit +3 more
core +2 more sources
Structural Biology of RNA and Protein‐RNA Complexes after AlphaFold3
ChemBioChem, Volume 26, Issue 7, April 1, 2025.AlphaFold is a great research accelerator and hypothesis generator for structural biology and beyond. With the release of AlphaFold3, RNA and protein‐RNA complex structure prediction is readily possible. This review assesses the reliability of these predictions.
Janosch Hennig
wiley +1 more source
Design and synthesis of probes for detection of protein-protein interaction and RNA localization [PDF]
, 2005 Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.Includes bibliographical references.The use of the ketone biotin - benzophenone-biotin hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB ...
Ryan, Jeremy Adam
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Decoupling Activity and Specificity in Coronazymes
Small, Volume 21, Issue 14, April 9, 2025.A new Au nanoparticle (AuNP) based nanozyme, aptamer–coronazyme, which demonstrates ≈30 times higher activity on d‐glucose than the pristine AuNP nanozymes, is designed and characterized. This coronazyme shows long‐range chiral specificity controlled by circularly polarized lights and catalytic reactivity modulated by short‐range intermolecular forces.
Jiahao Ji +6 more
wiley +1 more source
Small, Volume 21, Issue 9, March 5, 2025.Microfluidic‐based directed evolution is a powerful tool for selection of biopolymers, however they struggle in transferability to intracellular environments. The “biomimetic equivalence” principle provides a versatile and practical framework for developing biopolymers that function effectively in cells using a cell‐free approach.
Andrew Brian Kinghorn +10 more
wiley +1 more source
Effective suppression of Dengue virus using a novel group-I intron that induces apoptotic cell death upon infection through conditional expression of the Bax C-terminal domain [PDF]
, 2014 Introduction: Approximately 100 million confirmed infections and 20,000 deaths are caused by Dengue virus (DENV) outbreaks annually. Global warming and rapid dispersal have resulted in DENV epidemics in formally non-endemic regions.
Carter, James R. +7 more
core +1 more source
Physiologia Plantarum, Volume 177, Issue 2, March/April 2025.Abstract Viral infections are major contributors to crop yield loss and represent a significant threat to sustainable agriculture. Plants respond to virus attacks by activating sophisticated signalling cascades that initiate multiple defence mechanisms.
Kritika Shukla +4 more
wiley +1 more source
Nucleic acid enzymes for cleaving DNA [PDF]
, 2001 The present invention discloses nucleic acid enzymes capable of cleaving single-stranded DNA in a site specific ...
Joyce, Gerald F.
core +1 more source