Results 131 to 140 of about 942,703 (254)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Core circadian clock and light signaling genes brought into genetic linkage across the green lineage. [PDF]

Plant Physiol, 2022
Michael TP.
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Arabidopsis PIEZO Regulates Root Growth in Response to Magnetic Field and Blue Light Signaling. [PDF]

Physiol Plant
Peng Z, Yang W, Dong M, Bai H, Zhao W, Pan N, Lei Y, Zhu Z, Wan Y, Xie Y, Guo L, Peng S, Liu C, Du Y.   +13 more
europepmc   +1 more source

MET1-Dependent DNA Methylation Represses Light Signaling and Influences Plant Regeneration in Arabidopsis. [PDF]

Mol Cells, 2021
Shim S, Lee HG, Seo PJ.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Transcriptomic and metabolomic analyses reveal <i>OsACL-A2</i> coordinates light signaling, stress responses, and metabolic changes in rice. [PDF]

Front Plant Sci
Chen Z, Kong L, Liu H, Li G, Tian D, Wang Y, Lin Y, Yan J, Guo X, Hu C, Yang S.   +10 more
europepmc   +1 more source

CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum. [PDF]

Synth Syst Biotechnol, 2022
Wang Z, Zhao S, Zhang K, Lin C, Ru X, Yang Q.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Editorial: Hormonal Control of Important Agronomic Traits

Frontiers in Plant Science, 2018
Chi-Kuang Wen, Yunde Zhao, Yong-Ling Ruan   +2 more
doaj   +1 more source