Results 91 to 100 of about 1,174,395 (311)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Influence of Error Factors on Transmission Contact for Spiral Bevel Gears

open access: yesJixie chuandong, 2023
The full tooth flank contact characteristic of the line contact spiral bevel gear transmission is sensitive to errors. To obtain the influence of error factors on transmission contact for line contact spiral bevel gears, the contact simulation analysis ...
Wang Mingyang, Li Shuwen, Sun Yuehai
doaj  

Lines on contact Manifolds IIb

open access: yes, 2001
Fixed a number of minor issues found by the referee. To appear in Compositio Math. A PDF-file with additional graphics is available on the internet at http://www.mi.uni-koeln.de/~kebekus/publications-e ...
openaire   +3 more sources

Ultrasonic Reflection for Measurement of Oil Film Thickness and Contact between Dissimilar Materials [PDF]

open access: yes, 2012
The contact between dissimilar materials occurs in many machine elements where one of the contacting parts is manufactured from low modulus materials such as lip seals, o-rings, and metal on polymer prosthetic hip joints.
Gasni, Dedison
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

齿轮线接触与点接触理论与有限元分析

open access: yesJixie chuandong, 2014
The gear contact stress is very difficult to calculate and test owing to complexity of gear pairs contact condition by manufacturing error,assembly error,support conditions,deformation of loaded gear pairs,etc.The gear contact can be constituted of line ...
徐恺   +4 more
doaj  

The role of the microscale contact line dynamics in the wetting behaviour of complex fluids

open access: yesArchives of Mechanics, 2015
The microscale morphology of the liquid-substrate contact line is studied experimentally in two well-known examples of dynamic wetting with complex fluids (namely, drop impact of dilute polymer solutions and spreading of superspreader surfactant ...
D. Biolè, V. Bertola
doaj   +1 more source

Exact solution to a class of functional difference equations with application to a moving contact line flow

open access: yes, 1994
A new integral representation for the Barnes double gamma function is derived. This is canonical in the sense that solutions to a class of functional difference equations of first order with trigonometrical coefficients can be expressed in terms of the ...
Lawrie, JB, King, AC
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

open access: yesFEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani   +6 more
wiley   +1 more source

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