Results 251 to 260 of about 6,338,079 (289)

Systematic evaluation of line probe assays for the diagnosis of tuberculosis and drug-resistant tuberculosis

Clinica Chimica Acta, 2022
Line probe assays (LPAs) are PCR-based assays used for the rapid diagnosis of Mycobacterium tuberculosis (MTB) and drug-resistant tuberculosis (DR-TB). But studies on its performance are insufficient. Thus, in this study, we conducted a systematic review and meta-analysis to evaluate the effect of LPAs in the detection of MTB and drug-resistant TB in ...
Min, Lin, Ying-Wen, Chen, Yun-Ran, Li, Li-Jun, Long, Le-Yao, Qi, Ting-Ting, Cui, Shao-Yong, Wu, Jia-Yuan, Lin, Tong, Wu, Yi-Chen, Yang, Wei-Hua, Yuan, Ge-Yuan, Wu, Qi-Wen, Lan, Jia-Qi, Liu, Ya-Ping, Li, Zi-Yuan, Yu, Xu-Guang, Guo   +16 more
openaire   +2 more sources

Defining Threshold Values in Microscopic Examination and qPCR for Optimal Performance of Line Probe Assays for Resistance Detection in Mycobacterium tuberculosis Complex

Diagnostic Microbiology and Infectious Disease, 2019
Line probe assays enable rapid detection of drug-resistant Mycobacterium tuberculosis directly from clinical specimens. This study shows that defining threshold values in microscopic examination or PCR detection of M.
Bettina Schulthess, Peter M. Keller, Karoline Wagner   +2 more
exaly   +2 more sources

Role of line probe assay in detection of Mycobacterium tuberculosis in children with pulmonary tuberculosis

Indian Journal of Tuberculosis, 2023
India is the highest TB burden country in the world with almost 27 lakh cases reported in 2019. Pediatric tuberculosis in India accounts for almost 31% of global TB burden. Despite such high mortality and morbidity in children, diagnosis of pulmonary TB in children still remains very challenging.This cross-sectional study was conducted in a tertiary ...
Manisha, Bangarwa, Dhulika, Dhingra, Medha, Mittal, Karnika, Saigal, Arnab, Ghosh   +4 more
openaire   +2 more sources

Early line-probe assay using DNA specimens in patients with pulmonary TB

The International Journal of Tuberculosis and Lung Disease, 2022
BACKGROUND: We investigated the feasibility of early line-probe assay (LPA) using remnant DNA of Mycobacterium tuberculosis from polymerase chain reaction (PCR) test.METHODS: M. tuberculosis DNA specimens with cycle threshold (Ct) values reported and collected from patients with known results for both LPA with culture isolates and phenotype drug ...
A Y, Shin, D W, Jekarl, H W, Kim, J H, Ha, J H, Ahn, J S, Kim   +5 more
openaire   +2 more sources

Automatic Interpretation of Line Probe Assay Test for Tuberculosis

Proceedings of the AAAI Conference on Artificial Intelligence
Line Probe Assay (LPA) is a widely used method for diagnosing drug-resistant tuberculosis (DRTB), but it is a time-consuming and labor-intensive process that requires expert interpretation. DRTB is a significant threat to global TB control efforts and its prompt diagnosis is critical for initiating appropriate treatment.
Jatin Agrawal, Mukul Kumar, Avtansh Tiwari, Sachin Danisetty, Soma Dhavala, Nakul Jain, Prasaanth Balraj, Niket Singh, Siddhant Shingi, Jayakrishna Kurada, Raghuram Rao, S. Anand, Nishant Kumar   +12 more
openaire   +1 more source

Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and In-line Probing Assays

2013
Molecular docking calculations combined with chemically focused libraries can bring insight in the exploration of the structure-activity relationships for a series of related compounds against an RNA target. Yet, the in silico engine must be fueled by experimental observations to drive the research into a more effective ligand-discovery path.
Colizzi, Francesco, Lamontagne, A. M., Lafontaine, D. A., Bussi, Giovanni   +3 more
openaire   +3 more sources

HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II

2003
Hepatitis C viruses (HCVs) constitute a highly variable genus within the Flaviviridae, with closest homology to the hepatitis G and GB viruses, and Pestiviruses. The positive-stranded RNA genome encodes a polyprotein which is co- and posttranslationally cleaved into at least nine proteins.
G, Maertens, L, Stuyver
openaire   +2 more sources

Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase

2003
The human immunodeficiency virus type 1 (HIV-1) belongs to the family of positive-stranded, enveloped RNA viruses with a DNA intermediate step (retroviruses). Because of the lack of fidelity of the reverse transcriptase (RT), the replication is error-prone, and the infection is characterized by its quasi-species nature.
L, Stuyver, A, Wyseur, G, Verpooten, R, Rossau   +3 more
openaire   +2 more sources

Deferred standards, an on-line qualification, validation and system stability probe for chromatographic assay

Journal of Chromatography A, 2002
Specific programming of automated HPLC systems allows total on-line qualification, validation and stability monitoring using the concept of deferred standards. Setting up such a process for routine analyses in an automated HPLC system requires specific autosampler programming as well as specific monitoring software.
I, Clarot, M F, Dreyfuss, R, Domelier, S, Battu, P J P, Cardot   +4 more
openaire   +2 more sources

Rapid Detection of Rifampin-resistant Mycobacterium tuberculosis by Sequencing and Line Probe Assay

Scandinavian Journal of Infectious Diseases, 1998
Resistance to rifampin (RMP) is associated with mutations in the rpoB gene. Disk elution method, direct DNA sequencing and line probe assay were compared in rapid detection of rpoB mutations from 30 Mycobacterium tuberculosis isolates. Concordance between LiPA and culture was 93.3%, whereas sequencing yielded 100% concordance with culture.
H J, Marttila, H, Soini, B I, Vyshnevskiy, T F, Otten, A V, Vasilyef, P, Huovinen, M K, Viljanen   +6 more
openaire   +2 more sources