Results 41 to 50 of about 3,175,086 (283)
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Fuzzy rule interpolation for multidimensional input spaces with applications: A case study [PDF]
Fuzzy rule based systems have been very popular in many engineering applications. However, when generating fuzzy rules from the available information, this may result in a sparse fuzzy rule base.
Wong, K.W. +3 more
core
Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
A disposition of interpolation techniques [PDF]
A large collection of interpolation techniques is available for application in environmental research. To help environmental scientists in choosing an appropriate technique a disposition is made, based on 1) applicability in space, time and space-time, 2)
Heuvelink, G.B.M., Knotters, M.
core
Linear interpolation and Sobolev orthogonality
The authors study the connection between Sobolev orthogonality and simultaneous best approximation and interpolation on a certain Sobolev space. They give a symmetric bilinear form on the linear space of real polynomials and they study the relation between the orthogonal polynomials associated with this discrete continuous bilinear form and the ...
Samuel G. Moreno +1 more
openaire +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Pipeline geographic coordinate measurement error compensation and data preprocessing method
In order to improve the positioning accuracy of the geographic coordinate where the center line of long-distance oil and gas pipeline is located, the working principle of the pipeline geographic coordinate measurement system was analyzed, and the error ...
Liyuan GENG +4 more
doaj +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
An extended Prony’s interpolation scheme on an equispaced grid
An interpolation scheme on an equispaced grid based on the concept of the minimal order of the linear recurrent sequence is proposed in this paper.
Karalienė Dovile +3 more
doaj +1 more source

