Results 81 to 90 of about 1,084,218 (302)

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

Bio-Protocol, 2023
Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum, Huriye Uzun, Thomas Günther Pomorski   +2 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

A novel flow cytometric assay to quantify interactions between proteins and membrane lipids

Journal of Lipid Research, 2009
A diverse set of experimental systems has been developed to probe protein-lipid interactions. These include measurements with the headgroups of membrane lipids in solution, immobilized membrane lipids, and analysis of protein binding to membrane lipids ...
Koen Temmerman, Walter Nickel
doaj   +1 more source

How Well Can You Tailor the Charge of Lipid Vesicles? [PDF]

, 2019
Knowledge and control of surface charge or potential is important for tailoring colloidal interactions. In this work, we compare widely used zeta potential (ζ) measurements of charged lipid vesicle surface potential to direct measurements using the ...
Docto, D, Gilbile, D, Kingi, DT, Kuhl, TL, Kurniawan, J, Monahan, D, Tang, A   +6 more
core   +2 more sources

ER stress in antigen‐presenting cells promotes NKT cell activation through endogenous neutral lipids [PDF]

, 2020
CD1d-restricted invariant natural killer T (iNKT) cells constitute a common glycolipid-reactive innate-like T-cell subset with a broad impact on innate and adaptive immunity.
Cheng, Tan‐Yun, Cloots, Eva, De Geest, Bruno, Drennan, Michael, Elewaut, Dirk, Gaublomme, Djoere, Govindarajan, Srinath, Gysens, Fien, Janssens, Sophie, Maelegheer, Margaux, Moody, D Branch, Venken, Koen, Verheugen, Eveline   +12 more
core   +2 more sources

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

The anti-sepsis activity of the components of Huanglian Jiedu Decoction with high lipid A-binding affinity [PDF]

, 2017
Huanglian Jiedu Decoction (HJD), one of the classic recipes for relieving toxicity and fever, is a common method for treating sepsis in China. However, the effective components of HJD have not yet been identified.
Chen, Guirong, Jing, Jing, Li, Xuetao, Mackie, Brianna, Wang, Jing, Xu, Yubin, Zhang, Xu, Zheng, Xinchuan   +7 more
core   +2 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Structure and phospholipase function of peroxiredoxin 6: identification of the catalytic triad and its role in phospholipid substrate binding1

Journal of Lipid Research, 2007
Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase and phospholipase A2 (PLA2) activities, and it alone among mammalian peroxiredoxins can hydrolyze phospholipids.
Yefim Manevich, Konda S. Reddy, Tea Shuvaeva, Sheldon I. Feinstein, Aron B. Fisher   +4 more
doaj   +1 more source