Results 61 to 70 of about 2,675,614 (299)

Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins

STAR Protocols, 2022
Summary: Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins.
Zelun Zhi, Xuejing Ma, Chang Zhou, Adam Mechler, Shuyan Zhang, Pingsheng Liu   +5 more
doaj   +1 more source

Aromatic residues in the C terminus of apolipoprotein C-III mediate lipid binding and LPL inhibition

, 2017
Plasma apoC-III levels correlate with triglyceride (TG) levels and are a strong predictor of CVD outcomes. ApoC-III elevates TG in part by inhibiting LPL. ApoC-III likely inhibits LPL by competing for lipid binding.
Mikael Larsson, Larsson, Mikael, Donald M. Small, Olivecrona, Gunilla,, Evelina Vorrsjö, Gunilla Olivecrona, Meyers, Nathan L., Small, Donald M., Nathan L. Meyers, Vorrsjo, Evelina,   +9 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

C2 domain membrane penetration by group IVA cytosolic phospholipase A2 induces membrane curvature changes[S]

Journal of Lipid Research, 2012
Group IVA cytosolic phospholipase A2 (cPLA2α) is an 85 kDa enzyme that regulates the release of arachidonic acid (AA) from the sn-2 position of membrane phospholipids.
Katherine E. Ward, James P. Ropa, Emmanuel Adu-Gyamfi, Robert V. Stahelin   +3 more
doaj   +1 more source

Profiling of promoter occupancy by PPARalpha in human hepatoma cells via ChIP-chip analysis [PDF]

, 2010
The transcription factor peroxisome proliferator-activated receptor alpha (PPARalpha) is an important regulator of hepatic lipid metabolism. While PPARalpha is known to activate transcription of numerous genes, no comprehensive picture of PPARalpha ...
Degenhardt, Tatjana, de Groot, Philip, Merja Heinäniemi, Väisänen, Sami, de Groot, P.J., Carlberg, Carsten, Carsten Carlberg, de Vries, Sacco C, Müller, M.R., David L. M. van der Meer, Vries, Sacco, de, Philip J. de Groot, van der Meer, D.L., Degenhardt, T., Kersten, Sander, de Vries, Sacco, Heinaniemi, M., Vries, S.C., de, Kersten, A.H., Müller, Michael, Carlberg, C., Meer, D.L., van der, Sander Kersten, Michael Müller, Meer, D.L.M., van der, Groot, Philip, de, de Vries, S.C., Väisänen, S., Sami Väisänen, van der Meer, David L M, Groot, P.J., de, Heinäniemi, Merja, van der Meer, D.L.M., de Groot, Philip J, Tatjana Degenhardt, Sacco C. de Vries   +35 more
core   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Role of Cationic Side Chains in the Antimicrobial Activity of C18G

Molecules, 2018
Antimicrobial peptides (AMPs) have been an area of great interest, due to the high selectivity of these molecules toward bacterial targets over host cells and the limited development of bacterial resistance to these molecules throughout evolution.
Eric M. Kohn, David J. Shirley, Lubov Arotsky, Angela M. Picciano, Zachary Ridgway, Michael W. Urban, Benjamin R. Carone, Gregory A. Caputo   +7 more
doaj   +1 more source

YPIBP: A repository for phosphoinositide-binding proteins in yeast

Computational and Structural Biotechnology Journal, 2021
Phosphoinositides (PIs) are a family of eight lipids consisting of phosphatidylinositol (PtdIns) and its seven phosphorylated forms. PIs have important regulatory functions in the cell including lipid signaling, protein transport, and membrane ...
Jagat Rathod, Han-Chen Yen, Biqing Liang, Yan-Yuan Tseng, Chien-Sheng Chen, Wei-Sheng Wu   +5 more
doaj   +1 more source

Analysis of CD44-containing lipid rafts: Recruitment of annexin II and stabilization by the actin cytoskeleton [PDF]

, 1999
CD44, the major cell surface receptor for hyaluronic acid (HA), was shown to localize to detergent-resistant cholesterol-rich microdomains, called lipid rafts, in fibroblasts and blood cells.
Ursula Günthert, Günthert, U., Huber, L. A., Beug, H., Hartmut Beug, Gerke, V., Thomas Harder, Paiha, K., Volker Gerke, Oliferenko, S., Schwarz, H., Karin Paiha, Heinz Schwarz, Harder, T., Snezhana Oliferenko, Lukas A. Huber, Christoph Schwärzler, Schwärzler, C.   +17 more
core   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source