Results 181 to 190 of about 3,490 (194)

Asparagusate dehydrogenases and lipoyl dehydrogenase from asparagus mitochondria

Biochimica et Biophysica Acta (BBA) - Enzymology, 1975
1. Lipoyl dehydrogenase (NADH: lipoamide oxidoreductase, ED 1.6.4.3) and two asparagusate dehydrogenases from asparagus mitochondria were purified by a series of steps, freezing and thawing, sodium dodecylsulfate extraction, and chromatography on Sephadex G-200 and DEAE-cellulose. 2.
H, Yanagawa, F, Egami
openaire   +2 more sources

Characterization of Lipoyl Synthase from Mycobacterium tuberculosis

Biochemistry, 2016
The prevalence of multiple and extensively drug-resistant strains of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is on the rise, necessitating the identification of new targets to combat an organism that has infected one-third of the world's population, according to the World Health Organization.
Nicholas D, Lanz, Kyung-Hoon, Lee, Abigail K, Horstmann, Maria-Eirini, Pandelia, Robert M, Cicchillo, Carsten, Krebs, Squire J, Booker   +6 more
openaire   +2 more sources

Two redundant octanoyltransferases and one obligatory lipoyl synthase provide protein‐lipoylation autonomy to plastids of Arabidopsis

Plant Biology, 2013
AbstractOctanoyltransferases (LIP2) are important for the lipoylation of several α‐ketoacid decarboxylases and glycine decarboxylase, all of which are essential multienzyme complexes of central metabolism, by attaching de novo‐synthesised octanoyl moieties to the respective target subunits.
R, Ewald, C, Hoffmann, E, Neuhaus, H, Bauwe   +3 more
openaire   +2 more sources

Octanoylation of the lipoyl domains of the pyruvate dehydrogenase complex in a lipoyl‐deficient strain of Escherichia coli

Molecular Microbiology, 1990
SummaryThe overexpression of a subgene encoding a hybrid lipoyl domain of the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex of Escherichia coli has previously bee shown to result in the formation of lipoylated an unlipoylated products.
S T, Ali, A J, Moir, P R, Ashton, P C, Engel, J R, Guest   +4 more
openaire   +2 more sources

[32] Assay for protein lipoylation reaction

1995
Publisher Summary This chapter discusses the assay for protein lipoylation reaction. Lipoate attaches to the ɛ -amino group of the specific lysine residue of the proteins via an amide linkage. The lipoyllysine residue functions as a carrier of intermediates of the reactions and reducing equivalents between the active sites of the components of the ...
Kazuko Fujiwara, Kazuko Okamura-Ikeda, Yutaro Motokawa   +2 more
openaire   +1 more source

A colorimetric method for the assay of lipoyl dehydrogenase

Mikrochimica Acta, 1975
A colorimetric method for the direct assay of lipoyl dehydrogenase is described. Enzyme reaction is stopped by ethanol precipitation. This is followed by the displacement of 1,3-bis(2′-pyridyl)-1,2-diaza-prop-2-ene (PAPHY) from a Pd(II)-PAPHY complex by reduced lipoic acid.
A M, Seet, K T, Lee
openaire   +2 more sources

Characterization of a Radical Intermediate in Lipoyl Cofactor Biosynthesis

Journal of the American Chemical Society, 2015
Lipoyl synthase (LipA) catalyzes the final step in the biosynthesis of the lipoyl cofactor, the insertion of two sulfur atoms at C6 and C8 of an n-octanoyl chain. LipA is a member of the radical S-adenosylmethionine (SAM) superfamily of enzymes and uses two [4Fe-4S] clusters to catalyze its transformation.
Nicholas D, Lanz, Justin M, Rectenwald, Bo, Wang, Elizabeth S, Kakar, Tatiana N, Laremore, Squire J, Booker, Alexey, Silakov   +6 more
openaire   +2 more sources

[52] Lipoyl dehydrogenase from pig heart

1966
Publisher Summary This chapter discusses the determination of lipoyl dehydrogenase from pig heart. Three suitable assays described are: reduced diphosphopyridine nucleotide (DPNH)–lipoyl reductase activity, DPNH–dichlorophenolindophenol reductase activity, and DPNH–ferricyanide reductase activity.
openaire   +1 more source

On the reaction mechanism of lipoyl dehydrogenase

Biochimica et Biophysica Acta, 1960
V, MASSEY, C, VEEGER
openaire   +2 more sources

Lipoyl synthase

2009
openaire   +1 more source