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Lipoylated Peptides and Proteins
2015Lipoic acid is a heterocyclic sulfur-containing derivative of octanoic acid that is characterized by a 1,2-dithiolane ring. Found in nature as an essential protein cofactor, lipoic acid is implicated in cellular metabolic reactions. In particular, lipoylation is an essential posttranslational modification involved in cellular energetic metabolism ...
Rentier, Cédric +4 more
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Characterization of a Radical Intermediate in Lipoyl Cofactor Biosynthesis
Journal of the American Chemical Society, 2015Lipoyl synthase (LipA) catalyzes the final step in the biosynthesis of the lipoyl cofactor, the insertion of two sulfur atoms at C6 and C8 of an n-octanoyl chain. LipA is a member of the radical S-adenosylmethionine (SAM) superfamily of enzymes and uses two [4Fe-4S] clusters to catalyze its transformation.
Nicholas D, Lanz +6 more
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[32] Assay for protein lipoylation reaction
Journal of Molecular Biology, 1995Publisher Summary This chapter discusses the assay for protein lipoylation reaction. Lipoate attaches to the ɛ -amino group of the specific lysine residue of the proteins via an amide linkage. The lipoyllysine residue functions as a carrier of intermediates of the reactions and reducing equivalents between the active sites of the components of the ...
Kazuko Fujiwara +2 more
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Investigating the chemistry of lipoyl synthase [PDF]
, 2008 The radical SAM protein lipoyl synthase (LipA) is essential for lipoic acid biosynthesis via sulfur insertions into the unactivated C6 and C8 centres of a protein-bound octanoyl group. Using an in vitro assay which makes use of a small peptide mimic of the protein substrate, it has now been shown at which carbon centre sulfur insertion first occurs ...
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Studying the Lipoyl Synthase mediated conversion of Octanoyl substrates to Lipoyl products [PDF]
, 2013 a-Lipoic acid is a cofactor used during oxidative metabolism reactions by several enzymes, including branched chain keto acid dehydrogenases, the glycine cleavage system, pyruvate dehydrogenase and a-ketoglutarate dehydrogenase. The lipoic acid is attached to the e-amino group of a specific lysine residue via an amide bond and acts as a carrier of acyl
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Plant Biology, 2013
AbstractOctanoyltransferases (LIP2) are important for the lipoylation of several α‐ketoacid decarboxylases and glycine decarboxylase, all of which are essential multienzyme complexes of central metabolism, by attaching de novo‐synthesised octanoyl moieties to the respective target subunits.
R, Ewald +3 more
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AbstractOctanoyltransferases (LIP2) are important for the lipoylation of several α‐ketoacid decarboxylases and glycine decarboxylase, all of which are essential multienzyme complexes of central metabolism, by attaching de novo‐synthesised octanoyl moieties to the respective target subunits.
R, Ewald +3 more
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Journal of Chromatography B: Biomedical Sciences and Applications, 1996
An HPLC lipoamidase (lipoyl-X hydrolase) assay method has been developed, which uses a novel fluorescent substrate, lipoyl-6-aminoquinoline (LAQ). LAQ is synthesized from lipoic acid and 6-aminoquinoline (AQ) through lipoyl chloride as an intermediate and is conveniently purified by washing with chloroform-methanol.
K, Yoshikawa +5 more
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An HPLC lipoamidase (lipoyl-X hydrolase) assay method has been developed, which uses a novel fluorescent substrate, lipoyl-6-aminoquinoline (LAQ). LAQ is synthesized from lipoic acid and 6-aminoquinoline (AQ) through lipoyl chloride as an intermediate and is conveniently purified by washing with chloroform-methanol.
K, Yoshikawa +5 more
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Asparagusate dehydrogenases and lipoyl dehydrogenase from asparagus mitochondria
Biochimica et Biophysica Acta (BBA) - Enzymology, 19751. Lipoyl dehydrogenase (NADH: lipoamide oxidoreductase, ED 1.6.4.3) and two asparagusate dehydrogenases from asparagus mitochondria were purified by a series of steps, freezing and thawing, sodium dodecylsulfate extraction, and chromatography on Sephadex G-200 and DEAE-cellulose. 2.
H, Yanagawa, F, Egami
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[31] Lipoyl disulfide reducing polymers
1979Publisher Summary This chapter describes the preparation of thiolated polymers capable of reducing S–S bonds. They are easily synthesized by the covalent attachment of lipoic acid to different insoluble matrices, such as Sephadex, Sepharose, cellulose, polyacrylamide, and polystyrene.
Marian Gorecki, Abraham Patchornik
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The role of lipoylation in mitochondrial adaptation to methionine restriction
BioEssaysAbstractDietary methionine restriction (MR) is associated with a spectrum of health‐promoting benefits. Being conducive to prevention of chronic diseases and extension of life span, MR can activate integrated responses at metabolic, transcriptional, and physiological levels.
Jingyuan Xue, Cunqi Ye
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