Results 71 to 80 of about 4,253 (187)

Growth and lipoylation of Lpa constructs containing only the amidase domain.

, 2013
(A) Growth assay of E. coli cells expressing Lpat471 (closed triangles) and Lpat521 (open squares) compared to expression of Lpa (open triangles) and vector alone (open circles).
Sean T. Prigge (370088), Maroya D. Spalding (370087)   +1 more
core   +1 more source

Structural basis for catalysis by human lipoyl synthase

Nature Communications
Abstract Lipoic acid is an essential cofactor in five mitochondrial multiprotein complexes. In each complex, it is tethered in an amide linkage to the side chain of a conserved lysyl residue on a lipoyl carrier protein or lipoyl domain to afford the lipoyl cofactor.
Olga A. Esakova, Douglas M. Warui, Syam Sundar Neti, John N. Alumasa, Squire J. Booker   +4 more
openaire   +2 more sources

Pan-cancer analysis of the upstream regulator FDX1 in cuproptosis

Discover Oncology
The global incidence and mortality of cancer continue to rise rapidly, and cancer remains one of the most severe challenges in the field of public health.
Yan Xue, Qiong Tang, Wu Liu, Baoqing Zhao, Wanwan Jia   +4 more
doaj   +1 more source

Using Lipoamidase as a Novel Probe To Interrogate the Importance of Lipoylation in Plasmodium falciparum

mBio, 2018
Lipoate is a redox active cofactor that is covalently bound to key enzymes of oxidative metabolism. Plasmodium falciparum is auxotrophic for lipoate during the intraerythrocytic stages, but it is not known whether lipoate attachment to protein is ...
Hugo Jhun, Maroya S. Walters, Sean T. Prigge   +2 more
doaj   +1 more source

FDX1-mediated cuproptosis promotes cholestatic liver injury exacerbated by taurocholic acid-enhanced copper accumulation

Cell Death Discovery
Cholestatic liver injury, characterized by direct exposure of hepatocytes to retained bile components with elevated concentrations, represents a common manifestation of various hepatobiliary disorders with persistent threats to long-term patient survival
Yujun Guo, Min Yang, Shengbo Sun, Zhaohua Zhong, Wenjun Lu, Ze’nan Zhang, Meili Fan, Aodan Zhang, Tingting Zhang, Yang Wu, Zhou Li, Zuwei Liu, Qijun Sun, Zhaozhu Li, Qingbo Cui   +14 more
doaj   +1 more source

Growth, lipoylation, and biotinylation of cells expressing Lpa and Lpa active site mutants.

, 2013
(A) Growth assay of E. coli BL21 cells expressing Lpa (open triangles) compared to vector alone (open circles) and the three active site mutants: Lpa K159A (open squares), Lpa S235A (closed circles), and Lpa S259A (closed triangles).
Sean T. Prigge (370088), Maroya D. Spalding (370087)   +1 more
core   +1 more source

Understanding the Dynamics of Protein Lipoylation in Staphylococcus Aureus [PDF]

, 2018
Staphylococcus aureus is a commensal bacterium that also acts as an opportunistic pathogen. The pathogenicity of S. aureus has often been attributed to the wide range of virulence factors that the bacterium produces. While virulence factors do contribute
Flury, Sarah C
core   +1 more source

Copper-induced cell death mechanisms and their role in the tumor microenvironment

Frontiers in Cell Death
Copper is an essential cofactor for all organisms. However, it can become toxic if its concentration rises above a specific level. This level is controlled by evolutionary conserved homeostatic mechanisms.
Yujuan Jiao, Hongxin Wang, Meng Zhu, Tengteng Liu, Yuqi Li, Shuo Yang, Minghui Zhang, Lei Zhang   +7 more
doaj   +1 more source

Lipoylation and assembly of a 2-oxoacid dehydrogenase multienzyme complex from thermoplasma acidophilum [PDF]

, 2009
Energy generating processes like the citric acid cycle are a pivotal part of metabolism. Members of the 2-oxoacid dehydrogenase multienzyme complex (OADHC) superfamily feed into and act within the citric acid cycle.
Posner, Mareike
core  

Assessing the prognostic and therapeutic value of cuproptosis-related genes in colon adenocarcinoma patients

Frontiers in Cell and Developmental Biology
BackgroundColon adenocarcinoma (COAD) remains a major global health challenge with poor prognosis despite advances in treatment, underscoring the need for new biomarkers. As a novel mode of cell death, cuproptosis is thought to be potentially involved in
Zhanhui Ye, Yixian Song, Mengqing Zhu, Fuying Zheng, Wenjie Qin, Wenjie Qin, Xue Li, Pei Wang, Zihua Li, Kequan Chen, Aimin Li   +10 more
doaj   +1 more source