Results 111 to 120 of about 9,593,679 (252)

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Validating graft and standard liver size predictions in right liver living donor liver transplantation

, 2009
A formula was derived from 159 living liver donors to estimate the liver size of a normal subject in our previous study. Standard liver weight (g) = 218 + body weight (kg) x 12.3 + 51 (if male). The standard liver volume (SLV) is attained by a conversion
Lo, CM, Wong, Y, Ng, KKC, Chok, KSH, Fan, ST, Chan, SC   +5 more
core  

Gallstone Disease Does Not Predict Liver Histology in Nonalcoholic Fatty Liver Disease

, 2014
Background/Aims: We sought to examine whether the presence of gallstone disease (GD) in patients with biopsy-proven nonalcoholic fatty liver disease (NAFLD) is associated with liver fibrosis and histological nonalcoholic steatohepatitis (NASH) score ...
YILMAZ, YUSUF
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Improvement of hepatic reserve and changes in liver and spleen volume after balloon-occluded retrograde transvenous obliteration

BMC Gastroenterology
Background and aims Improvement in hepatic reserve after balloon-occluded retrograde transvenous obliteration (BRTO) in patients with gastric varices (GVs) has not been fully established. The relationship between increases in liver volume (LV) or splenic
Kazuma Shinkai, Ryotaro Sakamori, Ryoko Yamada, Yuki Tahata, Kazuki Maesaka, Kengo Matsumoto, Yasutoshi Nozaki, Seiichi Tawara, Hisashi Ishida, Yuichi Yoshida, Satoshi Tanaka, Toshifumi Ito, Yoshinori Doi, Sadaharu Iio, Mitsuru Sakakibara, Fumihiko Nakanishi, Kazuhiro Murai, Yoshinobu Saito, Akira Nishio, Kunimaro Furuta, Takahiro Kodama, Hayato Hikita, Tomohide Tatsumi, Tetsuo Takehara   +23 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Liver resection versus liver transplantation as treatment of early hepatocellular carcinoma: a case-control study

, 2009
Background and aim. The choice of the best surgical strategy for early hepatocellular carcinoma (HCC) on well-compensated cirrhosis is still controversial.
Iemmolo RM, Rompianesi G, Di Benedetto F, Gerunda GE, Masetti M, De Ruvo N, Cautero N, Guerrini GP, Ballarin R, Montalti R, Serra V   +10 more
core  

The interaction of genetics and physical activity in the pathogenesis of metabolic dysfunction associated liver disease

Scientific Reports
Genetic variants associated with increased liver fat and volume have been reported, but whether physical activity (PA) can attenuate the impact of genetic susceptibility to these traits is poorly understood.
Hanna Frostdahl, Nouman Ahmad, Ulf Hammar, Andrés Martínez Mora, Taro Langner, Tove Fall, Joel Kullberg, Håkan Ahlström, Hannah L. Brooke, Shafqat Ahmad   +9 more
doaj   +1 more source