Results 61 to 70 of about 296,746 (277)
Linked-read sequencing for detecting short tandem repeat expansions
Scientific Reports, 2022 Detection of short tandem repeat (STR) expansions with standard short-read sequencing is challenging due to the difficulty in mapping multicopy repeat sequences.
Readman Chiu +3 more
doaj +1 more source
FEBS Letters, EarlyView.Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai +4 more
wiley +1 more source
Read counts and mapping information.
, 2022 Read counts and mapping information.
David A. Matthews (542987) +11 more
core +1 more source
Chromosome-scale assembly and annotation of the perennial ryegrass genome
BMC Genomics, 2022 Background The availability of chromosome-scale genome assemblies is fundamentally important to advance genetics and breeding in crops, as well as for evolutionary and comparative genomics.
Istvan Nagy +7 more
doaj +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
First International Workshop, Rennes (Abstracts)
, 2016 Architectural Criticism between Public Debate and Autonomous Discipline First International Workshop ‘Mapping Architectural Criticism’, organized by Université Rennes 2 MONDAY, JANUARY 18th Introduction (Hélène Jannière, Université Rennes 2 ...
Mapping Architectural Criticism
core +1 more source
Correcting palindromes in long reads after whole-genome amplification
BMC Genomics, 2018 Background Next-generation sequencing requires sufficient DNA to be available. If limited, whole-genome amplification is applied to generate additional amounts of DNA.
Sven Warris +9 more
doaj +1 more source
Stranded Mapping from Long Reads v3 [PDF]
, 2018 This protocol demonstrates how to convert raw long reads produced using a strand-specific sequencing protocol (e.g. ONT's strand-switching protocol) into strand-specific mapped reads. The general ides is to use LAST to identify the adapter orientation relative to the genome, and then use that information to reorient called sequences to create a ...
openaire +1 more source
PBHoney: identifying genomic variants via long-read discordance and interrupted mapping [PDF]
BMC Bioinformatics, 2014 As resequencing projects become more prevalent across a larger number of species, accurate variant identification will further elucidate the nature of genetic diversity and become increasingly relevant in genomic studies. However, the identification of larger genomic variants via DNA sequencing is limited by both the incomplete information provided by ...
Adam C. English +2 more
openaire +2 more sources